The rejuvenating influence of young plasma on aged intestine

Abstract

This study aims to investigate the effects of plasma exchange on the biomolecular profiles and histology of ileum and colon tissues in young and aged Sprague–Dawley male rats. Fourier transform infrared (FTIR) spectroscopy, linear discriminant analysis and support vector machine (SVM) techniques were employed to analyse the lipid, protein, and nucleic acid indices in young and aged rats. Following the application of young plasma, aged rats demonstrated biomolecular profiles similar to those of their younger counterparts. Histopathological and immunohistochemical assessments showed that young plasma had a protective effect on the intestinal tissues of aged rats, increasing cell density and reducing inflammation. Additionally, the expression levels of key inflammatory mediators tumour necrosis factor‐alpha and cyclooxygenase‐2 significantly decreased after young plasma administration. These findings underscore the therapeutic potential of young plasma for mitigating age‐related changes and inflammation in the intestinal tract. They highlight the critical role of plasma composition in the ageing process and suggest the need for further research to explore how different regions of the intestines respond to plasma exchange. Such understanding could facilitate the development of innovative therapies targeting the gastrointestinal system, enhancing overall health during ageing.

Supplementing probiotics during intermittent fasting proves more effective in restoring ileum and colon tissues in aged rats

“…These perturbations potentially disrupt intestinal function, necessitating interventions to foster regeneration. 18 , 24 , 31 Our findings demonstrate that both IF and SCD Probiotics can alleviate these adverse changes associated with aging, indicating their potential as therapeutic strategies. The intestinal epithelium's primary food source is short‐chain fatty acids (SCFAs), produced by bacterial fermentation of complex carbohydrates in the gut.…”

“…Aging‐induced alterations in the ileum and colon tissues of rats, as observed in our study, manifested as increased epithelial shedding, decreased density of goblet and Paneth cells, heightened inflammation, and morphometric changes. These perturbations potentially disrupt intestinal function, necessitating interventions to foster regeneration 18,24,31 . Our findings demonstrate that both IF and SCD Probiotics can alleviate these adverse changes associated with aging, indicating their potential as therapeutic strategies.…”

“… 21 , 22 , 23 This technique has been used to analyse various biological samples such as blood, urine, and tissue, providing accurate and non‐invasive diagnosis of diseases such as cancer, diabetes, and cardiovascular disease. 24 , 25 Overall, IR spectroscopy is a powerful and promising technique that has the potential to revolutionize the field of medical diagnosis and treatment, offering non‐invasive, fast, and accurate analysis of biological samples. 26 …”

Rejuvenation of young blood on aging organs: Effects, circulating factors, and mechanisms

“…HPB has demonstrated its potential in preventing the age-related decline in pancreatic β-cell replication [ 50 ]. Furthermore, elderly rats that received young blood plasma exhibited a decrease in lymphocyte infiltration in the intestine [ 51 ]. In the case of adipose tissue, HPB has shown to alleviate senescence and inflammation in aging mice [ 52 ].…”

Exploring the therapeutic potential of plasma from intermittent fasting and untreated rats on aging‐induced liver damage

“…The study revealed rejuvenating effects on aged ileum and colon tissues, suggesting a therapeutic potential for intestinal aging. 14 …”