The Relation between the Persistence of El Tor Vibrio in the Intestines of Germfree Mice and So-called Coproantibody

Sasaki, Satoshi; Onishi, Nobuhiko; Suzuki, Ryoko; Adachi, K.; Miyashita, M.; Shimamura, Tadakatsu; Tazume, Seiki; Maeda, Ryozo; Takahashi, Toshiko

doi:10.1093/infdis/121.supplement.s124

Cited by 13 publications

(3 citation statements)

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“…Instead, we used adult GF mice to study HaitiV immunogenicity. In contrast to normal adult mice, which are resistant to V. cholerae intestinal colonization, oral inoculation of GF mice with V. cholerae results in stable intestinal colonization without adverse effects [35–37]. In the GF model, serum markers of immunity, such as vibriocidal titers, can be measured, but challenge studies are not possible due to the persistent colonization of the vaccine strain and the resistance of adult mice to diarrheal disease.…”

Section: Resultsmentioning

confidence: 99%

Oral immunization with a probiotic cholera vaccine induces broad protective immunity againstVibrio choleraecolonization and disease in mice

Sit

Zhang

Fakoya

et al. 2019

Preprint

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23Oral cholera vaccines (OCVs) are being increasingly employed, but current killed formulations 24 generally require multiple doses and lack efficacy in young children. We recently developed a 25 new live-attenuated OCV candidate (HaitiV) derived from a Vibrio cholerae strain isolated during 26 the 2010 Haiti cholera epidemic. HaitiV exhibited an unexpected probiotic-like activity in infant 27 rabbits, preventing intestinal colonization and disease by wild-type V. cholerae before the onset 28 of adaptive immunity. However, it remained unknown whether HaitiV would behave similarly to 29 other OCVs to stimulate adaptive immunity against V. cholerae. Here, we orally immunized adult 30 germ-free female mice to test HaitiV's immunogenicity. HaitiV safely and stably colonized 31 vaccinated mice and induced known adaptive immune correlates of cholera protection within 14 32 days of administration. Pups born to immunized mice were protected against lethal challenges of 33 both homologous and heterologous V. cholerae strains. Cross-fostering experiments revealed 34 that protection was not dependent on vaccine colonization in or transmission to the pups. These 35 findings demonstrate the protective immunogenicity of HaitiV and support its development as a 36 new tool for limiting cholera. 37 38

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Section: Resultsmentioning

confidence: 99%

Oral immunization with a probiotic cholera vaccine induces broad protective immunity againstVibrio choleraecolonization and disease in mice

Sit

Zhang

Fakoya

et al. 2019

Preprint

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“…An excellent illustration of the use of gnotobiotic animals in the elucidation of host-flora and interflora relationships is provided by Sasaki et al (283) who studied the persistence and antigenicity of V. cholerae and Shigella in relationship to superinfection with E. coli, S. faecalis, and C. welchil in the intestine of gnotobiotic mice. The "superinfectants," representing elements of the normal flora in mice, were found to coexist with one another, but antagonism occurred between them and the pathogenic organisms.…”

Section: Oral Diseasesmentioning

confidence: 99%

The gnotobiotic animal as a tool in the study of host microbial relationships.

Gordon¹,

Pesti²

1971

Bacteriological Reviews

270

121

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“…Although germfree mice have been used to study the chemotaxis and adhesion of V. cholerae to the intestinal mucosa in vivo (7), few prior studies have utilized a germfree mouse model of V. cholerae colonization to assess immune responses to infection; all have kept the animals germfree throughout the course of experimentation (9,10,13,15,16). In one study (13), 3-to 5-week-old germfree mice were inoculated with 10 6 CFU of classical V. cholerae.…”

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Development of a germfree mouse model of Vibrio cholerae infection

et al. 1996

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A mouse model of Vibrio cholerae infection was successfully developed with germfree mice. Three-to four-week-old germfree mice were orally inoculated with strains of V. cholerae to be tested and then moved to normal housing after inoculation. Stool culture, measurement of serum vibriocidal antibody titers, and determination of immune responses to the cholera toxin B subunit demonstrated that germfree mice are readily colonized by V. cholerae and develop systemic and mucosal immune responses to antigens expressed by these organisms. Immune responses to the B subunit of Shiga toxin 1, which was expressed from a V. cholerae vaccine vector, were less pronounced. This model should be valuable for studying immune responses to V. cholerae infection and immunization, including responses to heterologous antigens expressed by cholera vector strains.

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The Relation between the Persistence of El Tor Vibrio in the Intestines of Germfree Mice and So-called Coproantibody

Cited by 13 publications

References 0 publications

Oral immunization with a probiotic cholera vaccine induces broad protective immunity againstVibrio choleraecolonization and disease in mice

Oral immunization with a probiotic cholera vaccine induces broad protective immunity againstVibrio choleraecolonization and disease in mice

The gnotobiotic animal as a tool in the study of host microbial relationships.

Development of a germfree mouse model of Vibrio cholerae infection

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