The relation between turgor and tip growth inSaprolegnia ferax: Turgor is necessary, but not sufficient to explain apical extension rates

Kaminskyj, Susan G. W.; Garrill, Ashley; Heath, I. Brent

doi:10.1016/0147-5975(92)90042-p

Cited by 68 publications

(51 citation statements)

References 34 publications

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“…Intrinsic variations in hyphal extension rates have been recorded previously and correlated with such developmental events as mitosis and septum formation (36,37), the "cell cycle" (11,16,35), rhythmic colony growth and sporulation (28,37), and dichotomous branching (25,28). Erratic variation in growth rate was reported for S. ferax (25,29). However, all of these fluctuations were measured on a much longer time scale and thus cannot be directly compared, nor do they contribute to an explanation of the rapid elongation pulses we measured.…”

Section: Discussionmentioning

confidence: 99%

Pulsed growth of fungal hyphal tips.

López-Franco

Bartnicki-García

Bracker

1994

Proc. Natl. Acad. Sci. U.S.A.

141

115

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Section: Discussionmentioning

confidence: 99%

Pulsed growth of fungal hyphal tips.

López-Franco

Bartnicki-García

Bracker

1994

Proc. Natl. Acad. Sci. U.S.A.

141

115

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“…Many walled cells maintain their turgor by the biosynthesis of osmotically active metabolites (Jennings, 1995;Bohnert & Jensen, 1996) and ion uptake (Lew et al, 2006). Turgor is believed to drive cellular expansion, and intrahyphal pressure gradients may move cytoplasm forward with the growing tip (Lew, 2005), but not all hyphal organisms regulate turgor (Kaminskyj et al, 1992;Lew et al, 2004), and there are examples of amoeboid fungal cells (the slime mutant of N. crassa) and oomycetes (Money & Harold, 1993) that grow in the absence of turgor. Thus, tip-growing organisms can access alternative pathways to growth and morphogenesis, allowing longterm survival.…”

Section: Discussionmentioning

confidence: 99%

Transient responses during hyperosmotic shock in the filamentous fungus Neurospora crassa

Lew

Nasserifar

2009

Microbiology

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Fungal cells maintain an internal hydrostatic pressure (turgor) of about 400-500 kPa. In the filamentous fungus Neurospora crassa, the initial cellular responses to hyperosmotic treatment are loss of turgor, a decrease in relative hyphal volume per unit length (within 1 min) and cell growth arrest; all recover over a period of 10-60 min due to increased net ion uptake and glycerol production. The electrical responses to hyperosmotic treatment are a transient depolarization of the potential (within 1 min), followed by a sustained hyperpolarization (after 4 min) to a potential more negative than the initial potential (a driving force for ion uptake). The nature of the transient depolarization was explored in the context of other transient responses to hyperosmotic shock, to determine whether activation of a specific ion permeability or some other rapid change in electrogenic transport was responsible. Changing the ionic composition of the extracellular medium revealed that K + permeability increases and H + permeability declines during the transient depolarization. We suggest that these changes are due to concerted inhibition of the electrogenic H + -ATPase, and an increase in a K + conductance. Knockout mutants of known K + (tok, trk, trm-8, hak-1) and Cl " (a clc-3 homologue) channels and transporters had no effect on the transient depolarization, but trk and hak-1 do play a role in osmoadaptation, as does a homologue of a serine kinase regulator of H + -ATPase in yeast, Ptk2.

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“…1. It is important to note that fungi have significant internal turgor pressure so that even slight perturbation of the cell wall results in fungal cell lysis (54,73,(118)(119)(120).…”

Section: Fungal Cell Wall Structurementioning

confidence: 99%