The relation between xyr1 overexpression in Trichoderma harzianum and sugarcane bagasse saccharification performance

SummaryLignocellulosic biomass, which mainly consists of cellulose, hemicellulose and lignin, is the most abundant renewable source for production of biofuel and biorefinery products. The industrial use of plant biomass involves mechanical milling or chipping, followed by chemical or physicochemical pretreatment steps to make the material more susceptible to enzymatic hydrolysis. Thereby the cost of enzyme production still presents the major bottleneck, mostly because some of the produced enzymes have low catalytic activity under industrial conditions and/or because the rate of hydrolysis of some enzymes in the secreted enzyme mixture is limiting. Almost all of the lignocellulolytic enzyme cocktails needed for the hydrolysis step are produced by fermentation of the ascomycete Trichoderma reesei (Hypocreales). For this reason, the structure and mechanism of the enzymes involved, the regulation of their expression and the pathways of their formation and secretion have been investigated in T. reesei in considerable details. Several of the findings thereby obtained have been used to improve the formation of the T. reesei cellulases and their properties. In this article, we will review the achievements that have already been made and also show promising fields for further progress.

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“…Recently, da Silva Delabona et al . () showed that a 26‐fold constitutive overexpression of xyr1 in another Trichoderma spp. ( T .…”

Section: Engineering Cellulase Gene Expressionmentioning

confidence: 99%

Genetic engineering of Trichoderma reesei cellulases and their production

Druzhinina

Kubicek

2017

Microbial Biotechnology

184

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“…RNA extraction, reverse transcription, and RT-qPCR were performed as reported previously ( Meng et al, 2020 ). The relative transcription of genes was calculated by the 2 –ΔΔCt method ( Livak and Schmittgen, 2001 ), with the β-tubulin gene as the internal reference gene for normalization ( da Silva et al, 2017 ). Two biological replicates and three technical replicates for each reaction were carried out.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 660 genes in the T. harzianum genome were predicted to function as transcriptional regulators, among which the transcripts of 57 genes changed significantly at 24 h, and five of these genes were up-regulated, whereas 52 genes were down-regulated (|Log 2 FC| > 1, p-adjust < 0.05) (Supplementary File S3). Besides the regulators Xyr1 (Th_1126) and Cre1 (Th_502975) reported to regulate cellulase production by T. harzianum (da Silva et al, 2017), the effects of other differentially expressed transcription factors potentially regulating cellulase induction in T. harzianum will be discussed below according to their homologues in T. reesei.…”

Section: Changed Genes That Encode Regulators and Major Transportersmentioning

confidence: 99%

Diversity of Cellulase-Producing Filamentous Fungi From Tibet and Transcriptomic Analysis of a Superior Cellulase Producer Trichoderma harzianum LZ117

Zhang

Jiang³

et al. 2020

Front. Microbiol.

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“…The primer pair TMLS-F/TMLS-R (Table S1) was designed for the detection of the total viral accumulation (ThHV1 + ThHV1-S), as it primed the RT-PCR amplification of a shared region in the genome of ThHV1 and ThHV1-S. Furthermore, the primer pair Tubulin-F/Tubulin-R (Table S1) was used for the specific detection of the β-tubulin gene of T. harzianum , which is considered as a constitutive expression gene in fungi, and can be used for justification of the sample-to-sample variation in the amount of RNA [33]. All the PCR amplifications were carried out in a Bio-Rad (Hercules, CA, USA) CFX96TM Real-Time PCR Detection System with SYBR ® Premix Ex Taq™ (TaKaRa).…”

Section: Methodsmentioning

confidence: 99%

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

et al. 2019

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Trichoderma species are a group of fungi which is widely distributed in major terrestrial ecosystems; they are also commonly used as biocontrol agents for many plant diseases. A virus, namely Trichoderma harzianum hypovirus 1 (ThHV1), was identified in T. harzianum isolate T-70, and also infected isolate T-70D, together with its defective RNA (ThHV1-S). The ThHV1 genome possessed two Open Reading Frames (ORFs), namely ORF1 and ORF2. The start codon of ORF2 overlapped with the stop codon of ORF1 in a 43 nt long region. The polypeptide encoded by ORF2 of ThHV1 shared sequence similarities with those of betahypoviruses, indicating that ThHV1 is a novel member of Hypoviridea. Isolate T-70D, carrying both ThHV1 and ThHV1-S, showed abnormal biological properties, notably a decreased mycoparasitism ability when compared with isolate T-70. Both ThHV1 and ThHV1-S could be vertically transmitted to conidia and horizontally transmitted to T. harzianum isolate T-68 and T. koningiopsis T-51. The derivative strains carrying both ThHV1 and ThHV1-S showed decreased mycoparasitism ability, whereas strains carrying ThHV1 alone were normal, indicating that ThHV1-S is closely associated with the decreased mycoparasitism ability of T. harzianum isolate T-70D. ThHV1 was widely detected in isolates of T. harzianum, T. koningiopsis and T. atroviride originating from soil of China. Therefore, viruses in fungal biocontrol agents may also be a factor associated with the stability of their application.

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The relation between xyr1 overexpression in Trichoderma harzianum and sugarcane bagasse saccharification performance

Cited by 32 publications

References 43 publications

Genetic engineering of Trichoderma reesei cellulases and their production

Genetic engineering of Trichoderma reesei cellulases and their production

Diversity of Cellulase-Producing Filamentous Fungi From Tibet and Transcriptomic Analysis of a Superior Cellulase Producer Trichoderma harzianum LZ117

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

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