The Relation of Hydrogen-Ion Concentration to the Penetration of 3-Indole Acetic Acid into Nitella Cells

Albaum, Harry G.; Kaiser, Samuel; Nestler, Herbert A.

doi:10.2307/2437073

Cited by 32 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Skoog (1938) reported that uptake of auxin by the roots of tomato plants was proportional to the concentration supplied. Albaum, Kaiser & Nestler (1937), who studied the effects of hydrogen-ion concentration on the penetration of indole-3-acetic acid into Nitella cells, found that the curve representing the velocity of penetration as a function of pH coincided with the dissociation curve for indole-3-acetic acid. They concluded that this substance entered in the form of undissociated molecules, and considered that diffusion fully accounted for their observations.…”

Section: Introductionmentioning

confidence: 97%

The Uptake Oe Indole‐3‐acetic Acid by Pea Epicotyl Segments and Carrot Disks

Reinhold

1954

New Phytologist

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 97%

The Uptake Oe Indole‐3‐acetic Acid by Pea Epicotyl Segments and Carrot Disks

Reinhold

1954

New Phytologist

View full text Add to dashboard Cite

“…Although precise calculations are not possible because insufficient * A" = generalized singly-charged anion; C"^ = generalized singly-charged cation; c -cytoplasmic phase (subscript); E = electrical potential of phase i with respect to phase o taken as zero; i = internal phase (subscript); o == external phase (subscript); P = permeability coefficient; R = gas constant; T = absolute temperature. data were provided to allow calculation of cell surface area, a similar value of PIAA seems likely for the data of Albaum, Kaiser and Nestler (1937), who used Nitella cells. A high value of PIAA is also suggested by the work of Rubery and Sheldrake (1973).…”

Section: Introductionmentioning

confidence: 99%

TRANSPORT OF INDOLEACETIC ACID IN PLANT CELLS IN RELATION TO pH AND ELECTRICAL POTENTIAL GRADIENTS, AND ITS SIGNIFICANCE FOR POLAR IAA TRANSPORT

1975

View full text Add to dashboard Cite

SUMMARYThe distribution of IAA between the vacuole and the bathing solution in Hydrodictyon africanum is consistent with passive entry of undissociated IAA and passive efflux of both undissociated IAA and of IAA", with Pi^^ (permeability coefficient)* about io~^ cms~^ and PIAA-about io~^ cms~^ The involvement of IAA~ in the efflux results from the inside-negative P.D. between the medium and the vacuole. The cytoplasm is at a higher pH than either the vacuole or the bathing solution used in most experiments; this is maintained by active H"*" efflux at the plasmalemma, and active influx at the tonoplast. In this situation the efflux of IAA" is further promoted by the increased concentration of IAA" in the relatively alkaline cytoplasm. Thus the apparent active efflux of IAA from these cells can be explained in terms of passive driving forces of concentration and electrical potential acting on IAA and IAA~, with the distribution of these two species dictated ultimately by PI^A, PIAA-> the pH of the various compartments and the electrical potential difference between them. If PIAA/^IAA-were larger at the apical than at the basal end of coleoptile cells, such an effect could explain polar IAA transport, with metabolic energy being used only to maintain the relative permeabilities to the two species, the pH gradient and the electrical gradient.

show abstract

“…Dolk and Thimann (6) showed that plant response to IAA was greater at low than at high pH, and Albaum et al (1) demonstrated that IAA penetration into Nitella cells was greater at pH 4 than at pH 7. Subsequent work has confirmed these early observations (22). Similar responses to pH have been reported for 2,4-D (3,9,17,19,21).…”

mentioning

confidence: 99%

Effect of pH on Penetration of Naphthaleneacetic Acid and Naphthaleneacetamide Through Isolated Pear Leaf Cuticle

Norris

Bukovac

1972

Plant Physiol.

View full text Add to dashboard Cite

Penetration of naphthaleneacetic acid through enzymatically isolated upper pear (Pyrus communis L. cv. Bartlett) leaf cuticle increased as the donor pH was decreased. Naphthaleneacetamide penetration was not influenced by donor pH. The effect of pH on naphthaleneacetic acid penetration was reversible. Higher receiver (simulated leaf interior) pH favored penetration of naphthaleneacetic acid. Changes in the degree of dissociation, and hence polarity, as controlled by hydrogen ion concentration was the prime factor in the response of naphthaleneacetic acid to pH. At pH values lower than the pK (4.2 for naphthaleneacetic acid), the molecule was primarily undissociated, lipophilic, and penetrated into the cuticle; whereas, at pH values above the pK naphthaleneacetic acid was ionized, hydrophilic, and penetrated the cuticle with difficulty or not at all. Data presented are consistent with the hypothesis that naphthaleneacetic acid and naphthaleneacetamide penetration through the cuticle takes place by diffusion.Hydrogen ion concentration influences the biological activity of weak organic acid type plant growth regulators (4,10,16,20). Dolk and Thimann (6) showed that plant response to IAA was greater at low than at high pH, and Albaum et al.(1) demonstrated that IAA penetration into Nitella cells was greater at pH 4 than at pH 7. Subsequent work has confirmed these early observations (22). Similar responses to pH have been reported for 2, 4-D (3, 9, 17, 19, 21). The effect of pH has been explained on the basis that at pH values below the pK undissociated molecules predominate and penetrate rapidly, whereas at high pH values most molecules are dissociated, and the dissociated ion penetrates with difficulty (3,17,18,20 MATERIALS AND METHODSCuticle Isolation and Penetration Assay. The astomatous cuticular membrane from the upper surface of pear (Pyrus communis L. cv. Bartlett) leaves was isolated enzymatically using 2.0% pectinase and 0.1% cellulase buffered at pH 3.8 as previously described (11). Discs of isolated cuticle were fixed to the end of a glass tube with buffered radioactive donor solution inside and with water or buffer on the outside as a receiver. Samples were withdrawn from the receiver, and the amount of radioactivity penetrated was determined using a Tracerlab thin end window gas flow counter. A detailed description is available elsewhere (12). Naphthaleneacetic-1-"C (NAA-'4C) acid and naphthaleneacetamide-1-"4C (NAAm-14C) were used at 0.5 mM, 1.0 mc/mmole. Purity of the labeled auxins was confirmed by thin layer chromatography. Buffers were prepared according to Goromi (8); citrate-phosphate or acetic acid-sodium acetate were employed unless otherwise noted. Throughout this work, donor refers to the initial NAA-4C (or NAAm-"'C) solution from which penetration occurred, and receiver to the solution into which penetration took place during the course of the experiment. All data are presented as nmoles of chemical penetrated over a period of 48 hr.Sorption Determination. Sorption was determined ...

show abstract

The Relation of Hydrogen-Ion Concentration to the Penetration of 3-Indole Acetic Acid into Nitella Cells

Cited by 32 publications

References 0 publications

The Uptake Oe Indole‐3‐acetic Acid by Pea Epicotyl Segments and Carrot Disks

The Uptake Oe Indole‐3‐acetic Acid by Pea Epicotyl Segments and Carrot Disks

TRANSPORT OF INDOLEACETIC ACID IN PLANT CELLS IN RELATION TO pH AND ELECTRICAL POTENTIAL GRADIENTS, AND ITS SIGNIFICANCE FOR POLAR IAA TRANSPORT

Effect of pH on Penetration of Naphthaleneacetic Acid and Naphthaleneacetamide Through Isolated Pear Leaf Cuticle

Contact Info

Product

Resources

About