The relation of peptide bonds, disulphide bonds and sulphydryl groups to urogastrone activity

The preparation of urogastrone

“…It has been shown that reagents which reduce disulphide bonds inactivate urogastrone (Rosenoer, 1962). Incubation of urogastrone with 50 mm sodium sulphite at pH 7-8 and 380 C for 90 min reduced the gastric inhibitory activity of the preparation to 37 (28-49) %.…”

“…At the most only a small fraction of the gastric inhibitory activity could be due to them. The possibility that the urogastrone activity may be due to adrenaline or some other sympathomimetic amine is further contradicted by the evidence reported elsewhere (Rosenoer, 1962) that urogastrone is a polypeptide destroyed by peptidases.…”

“…However, Ghosh (1959) has clearly differentiated between urogastrone and gonadotrophins by showing that the gonadotrophic activities of purified urinary gonadotrophins do not correspond to their gastric inhibitory activities. Our preparation also contains a heat-labile anti-tryptic substance (Rosenoer, 1962). Its heat lability distinguishes it from the heat-stable urogastrone.…”