The Relation of the Blood Level of Ascorbic Acid to the Tissue Concentrations of This Vitamin and to the Histology of the Incisor Teeth in the Guinea Pig

Kuether, Carl A.; Telford, Ira R.; Roe, Joseph H.

doi:10.1093/jn/28.5.347

Cited by 41 publications

(8 citation statements)

References 16 publications

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“…At high blood levels the plasma concentration is higher than the whole blood concentration; at intermediate blood levels the plasma and whole blood concentrations are approximately the same; and at low blood levels the plasma concentration is considerably lower than the whole blood concentration. Figure 3 (2). In normal human subjects it is well-accepted that the blood level of ascorbic acid parallels the dietary intake of the vitamin up to a point where massive doses are administered (10 to 14).…”

Section: Resultsmentioning

confidence: 99%

The Distribution of Ascorbic Acid in the Blood

Roe¹,

Kuether²,

Zimler³

1947

J. Clin. Invest.

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In this paper we are reporting the results of a study of the distribution of ascorbic acid in the blood in human subjects and in guinea pigs. EXPERIMENTALFifty healthy medical students (47 males and 3 females) on self-selected diets and 50 patients (41 males and 9 fe-. males) in a municipal hospital were studied. Oxalated blood samples were collected under fasting conditions about 15 hours post-prandial. The ascorbic acid con-,.tent of the whole blood and the plasma was determined by the method of Roe and Kuether (1). The concentration of ascorbic acid in oxalated whole blood and plasma was also determined by the same method in guinea pigs upon an apparently adequate diet (2) made up to contain different levels of ascorbic acid. RESULTS

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Section: Resultsmentioning

confidence: 99%

The Distribution of Ascorbic Acid in the Blood

Roe¹,

Kuether²,

Zimler³

1947

J. Clin. Invest.

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“…Details and modifications of these and other histological techniques used in this study are given elsewhere (27). The adrenals were removed immediately after death, the left one being used for determination of total and free cholesterol (28) and half of the right gland for the determination of ascorbic acid (29). The remaining half adrenal was fixed in 10 per cent formalin and frozen sections were stained with hematoxylin and eosin, oil-red-O for lipids, Schultz's technique for sterols, or examined unstained under polarized light for birefringent material.…”

Section: Methodsmentioning

confidence: 99%

Desoxycorticosterone Acetate and Wound Healing

Pirani

Stepto

Sutherland

1951

Journal of Experimental Medicine

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According to Selye and coworkers (1-3) overdosage of desoxycorticosterone acetate (DCA) in the rat may induce changes characterized by nephrosclerosis with hypertension and by cardiovascular and articular lesions of the "rheumatic type." On the basis of his experiments Selye (4, S) theorized that certain chronic diseases, such as rheumatic fever, periarteritis nodosa, arthritis, and hypertension, are the result of an abnormal response or of a perverted adaptation to stress mediated through the endocrine system, particularly an overproduction of adrenocortical hormones or hypercorticism. These conditions, which are all known to involve mainly tissues of mesenchymal origin, were therefore classified by Selye as "diseases of adaptation."Recently Taubenhaus and Amromin (6) reported that DCA stimulates the proliferation of fibroblasts and encourages the deposition of a homogeneous groundwork of collagen. They obtained these results by studying the effect of the hormone on the granulation tissue around subcutaneous turpentine abscesses in rats. On the basis of more recent studies (7) these investigators advanced the opinion that the stimulating effect of DCA may take place through mechanisms other than direct influence upon granulation tissue. The possibility that the action of DCA is mediated through an enzymatic mechanism must be considered especially in view of observations (8, 9) pointing to the important role of alkaline phosphatase in the proliferation of tissues of mesenchymal origin, and of other studies (10) indicating that DCA increases the activity of alkaline phosphatase in the epiphyses of rats' femora. The effect of DCA administration on other factors which influence mesenchymal cell proliferation, such as ascorbic acid metabolism and adrenocortical function, must also be considered in this respect.Previous unpublished studies from our laboratory (11) on the effect of large doses of DCA in intact guinea pigs showed that, as in the rat (4, 5), the main lesions attributable to this hormone are localized in the cardiovascular-renal system and in the adrenals. These changes consist of moderate to severe nephro-* The opinions expressed in this paper are those of the authors, and do not necessarily represent the official views of any governmental agency.

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“…Significant data and additional citations of the literature will be found in the recent references given, dealing primarily with maternal and infant feeding (86)(87)(88)(89)(90) requirements of children (91)(92)(93)(94), requirements of adults (95)(96)(97)(98)(99)(100)(101)(102)(103), distribution in human tissues (104), effects upon tooth structure (105)(106)(107), and the enzyme contents of wounds during healing (108).…”

Section: Riboflavinmentioning

confidence: 99%

Recommended dietary allowances

1955

The Journal of Pediatrics

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The Relation of the Blood Level of Ascorbic Acid to the Tissue Concentrations of This Vitamin and to the Histology of the Incisor Teeth in the Guinea Pig

Cited by 41 publications

References 16 publications

The Distribution of Ascorbic Acid in the Blood

The Distribution of Ascorbic Acid in the Blood

Desoxycorticosterone Acetate and Wound Healing

Recommended dietary allowances

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