The Relation of the Stippled Cell and the Polychromatic Cell to the Reticulocyte

Whitby, LionelE.H.; Britton, Cameron J.

doi:10.1016/s0140-6736(00)98470-8

Cited by 20 publications

(4 citation statements)

References 4 publications

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“…This view, originally put forward by Whitby and Britton (1933), has recently been confirmed by Jensen and his co-workers (1965) as a result of their optical and electron microscopic studies. Their findings showed that reticulocytes or stippled cells could be produced at will in films by varying the staining technique used and that both, as well as the diffuse basophilia in Romanowsky films, could be abolished by prior treatment with ribonuclease, concluding that all three types of cells were related in some way and all resulted from a ribosomal abnormality.…”

Section: Discussionmentioning

confidence: 63%

Screening lead workers for the presence of stippled erythrocytes

Clark

Jones

1967

J Clin Pathol

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SYNOPSISThe number of stippled erythrocytes in the blood of lead workers counted under dark ground microscopy show a marked fall when blood is taken into an anticoagulant. The significance of this finding is studied and alternative methods of screening workers are examined.It has long been recognized that the presence of red cells in the peripheral blood showing basophilic stippling is a feature of lead intoxication, and an assessment of their numbers has been widely used as an indication of excessive absorption by lead workers.A fortuitous finding that the high stippled cell counts in capillary blood films from lead workers were not seen in films prepared from venous blood taken into an anticoagulant seemed to be an observation worth recording in order to draw attention to the dangers of false low results in such specimens, and prompted an investigation into the phenomenon in an endeavour to throw some light on the nature and formation of stippled cells. MATERIALS AND METHODSIndividuals were selected from local industrial concerns who were exposed to lead oxide and showed a stippled cell count in excess of 6,000 per million red cells as estimated by the method suggested by Lane (1949). These could all be regarded as showing evidence of excessive lead absorption, the criterion of which, using this method, is a count greater than 5,000 per million red cells. Both capillary and venous blood was taken Received for publication 20 September 1966. from each individual and films were prepared immediately. In addition, films were made from the venous specimens after a varying period of time in a variety of anticoagulants.The dried films were, at first, stained with 0 5% alkaline methylene blue in accordance with Lane's method, but later 1 % new methylene blue was used, staining for 15 min., and subsequently washing off with tap water.The technique for reticulocyte staining was that suggested by Breecher (1949) using 1 % new methylene blue in citrate-saline as a vital stain.The serum haptoglobulin was estimated by the agar diffusion method of Rowe (1961). RESULTS

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Section: Discussionmentioning

confidence: 63%

Screening lead workers for the presence of stippled erythrocytes

Clark

Jones

1967

J Clin Pathol

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“…His studies resulted in the demonstration that the basophilic particles were both Feulgen-and iron-negative. Whitby & Britton (1933) pointed out that to Hawes (1909) belongs the credit for suggesting that both stippled and polychromatophilic mammalian erythrocytes are immature cells, a concept they also supported. Dustin (1943) noted that stippling as well as polychromasia as seen in Romanowsky-stained smears was eliminated by the digestive action of ribonuclease.…”

Section: Discussionmentioning

confidence: 98%

Erythrocyte basophilic stippling in the Mongolian gerbil

1976

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Basophilic stippling of the circulating erythrocytes is characteristic of the Mongolian gerbil. Its enzymatic digestion along with the concomitant removal of diffuse erythrocytic polychromasia by the action of ribonuclease demonstrates that it represents microscopically visible aggregates of cytoplasmic ribonucleoprotein, presumably of ribosomal origin. Up to 40% of the total circulating erythrocytes may be stippled in foetal and newborn animals. There is then a progressive decline in incidence until adult levels are attained at least by 20 weeks of age. The bone marrow of the adult gerbil contains a higher proportion of stippled red cells than the circulating blood. The stippling can be either coarse or fine and observable in both polychromatophilic and orthochromic cells. It is suggested that erythrocytes with basophilic stippling are relatively immature red cells still demonstrating remnants of cytoplasmic ribonucleoprotein.

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“…The work of Key (1924) did much to establish the present conception of the stippled cell as a young cell, and the stippling as a granular degeneration of the basophilic substance of the cytoplasm. The close association of punctate basophilia with polychromasia and with reticulation is now generally accepted (Whitby and Britton, 1933).…”

Section: Normal Absorption and Excretion Of Leadmentioning

confidence: 99%

The care of the lead worker. 1949.

Lane¹

1993

Occupational and Environmental Medicine

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The Relation of the Stippled Cell and the Polychromatic Cell to the Reticulocyte

Cited by 20 publications

References 4 publications

Screening lead workers for the presence of stippled erythrocytes

Screening lead workers for the presence of stippled erythrocytes

Erythrocyte basophilic stippling in the Mongolian gerbil

The care of the lead worker. 1949.

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