The Relation of the Tricarboxylic Acid Cycle to Bacterial Infection: II. The Effect of Fluoroacetate, Arsenite, Citrate, and Succinate on Salmonella Typhimurium Infections In Mice

Berry, L. Joe; Rb, Mitchell

doi:10.1093/infdis/93.1.83

Cited by 16 publications

(4 citation statements)

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“…This evidence coupled with the previous finding (Hill et al, 1959) that acute starvation also resulted in an increase in the concentration of this metabolite strongly suggested that this change in concentration was under the control of hormones asso- dated with the non-specific "stress" response, i.e. This finding is of potential importance, since it has been shown by Berry and Mitchell (1953) and Gilfillan et al (1956) that compounds which block the citric acid cycle render mice and chicks more susceptible to salmonella infection. An experiment was conducted, therefore, in which ACTH (Upjohn) was given both intravenously and intramuscularly and the blood citric acid concentration followed at intervals for 5 hours.…”

Section: Resultssupporting

confidence: 72%

Blood Citric Acid Concentration as Affected by Heat and Cold Stress and Adrenocorticotrophic Hormone

et al. 1961

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Section: Resultssupporting

confidence: 72%

Blood Citric Acid Concentration as Affected by Heat and Cold Stress and Adrenocorticotrophic Hormone

et al. 1961

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“…Impeding the TCA/Kreb's cycle, where the majority of NADH is created by reducing NAD + , induced changes distinct from those incurred by blocking other steps of metabolism. TCA cycle inhibition was performed by administering either the aconitase-blocker Sodium fluoroacetate [37] or Sodium meta-arsenite, which reportedly inhibits both pyruvate and α-ketoglutarate dehydrogenases [38,39]. Figure 3(c) shows that fluoroacetate and arsenite reagents induced significant reductions in the NADH intensity by 15% and 30%, respectively, relative to baseline.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo

Yaseen

Sutin

et al. 2017

Biomed. Opt. Express

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Evaluating cerebral energy metabolism at microscopic resolution is important for comprehensively understanding healthy brain function and its pathological alterations. Here, we resolve specific alterations in cerebral metabolism in vivo in Sprague Dawley rats utilizing minimally-invasive 2-photon fluorescence lifetime imaging (2P-FLIM) measurements of reduced nicotinamide adenine dinucleotide (NADH) fluorescence. Time-resolved fluorescence lifetime measurements enable distinction of different components contributing to NADH autofluorescence. Ostensibly, these components indicate different enzyme-bound formulations of NADH. We observed distinct variations in the relative proportions of these components before and after pharmacological-induced impairments to several reactions involved in glycolytic and oxidative metabolism. Classification models were developed with the experimental data and used to predict the metabolic impairments induced during separate experiments involving bicuculline-induced seizures. The models consistently predicted that prolonged focal seizure activity results in impaired activity in the electron transport chain, likely the consequence of inadequate oxygen supply. 2P-FLIM observations of cerebral NADH will help advance our understanding of cerebral energetics at a microscopic scale. Such knowledge will aid in our evaluation of healthy and diseased cerebral physiology and guide diagnostic and therapeutic strategies that target cerebral energetics. 2(2), 145-150 (1966). 33. L. K. Klaidman, A. C. Leung, and J. D. Adams, Jr., "High-performance liquid chromatography analysis of oxidized and reduced pyridine dinucleotides in specific brain regions," Anal.

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“…The reason decreased resistance occurs as a result of fasting has not been elucidated. However, one may surmise that the fasted animal undergoes metabolic changes similar to those described by Berry and Mitchell (3,4) in their work with tricarboxylic acid cycle inhibitors and Salmonella typkimcriura infections.…”

Section: Discussionmentioning

confidence: 80%

Studies on Experimental Shigellosis

1958

The Journal of Experimental Medicine

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Rats, mice, and other small rodents under natural conditions are refractory to disease of Shigella etiology. A previous publication in this series has described the symptoms and pathology which occurred in normal laboratory albino mice experimentally infected with Shigellaflexneri 3 (1).Experiments to be described in the present paper illustrate the effect of fasting and fatigue on the response of albino mice to experimental Shigella infections. In these experiments the response of the host has been estimated on the basis of the mortality rate among infected animals, on the persistence of the organisms in the intestine, and on the occurrence of bacteriemia in the experimental host. Materials and MethodsThe routine methods and materials employed in this study were the same as those described in an earlier publication (1). EXPERIMENTAL AND RESULTS The Effect of Fasting on Shigdla Infections in Mice.--Mice were fasted by withdrawing all food from their cages, but allowing free access to water. The cages were constructed of woven wire which denied the animals access to fecal droppings. In a few instances weaker animals were killed by their cage mates, or died before the completion of the fasting period. Mice were not used ff any deaths occurred among cage mates during the prefasting period. Fasted and non-fasted mice were used in parallel experiments to determine the LDso of S. flexnezi 3.The results presented in Table I indicate that the intraperitoneal and rectal LDs0 of Shigetla decreased as the length of fasting time increased. However, the oral LDs0 for fasted animals was larger than for normal animals. This is * The opinions or assertions contained herein are the private ones of the writers and are not to be construed as ofllcial or reflecting the views of the Navy Department or the naval service at large. ~/Present

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The Relation of the Tricarboxylic Acid Cycle to Bacterial Infection: II. The Effect of Fluoroacetate, Arsenite, Citrate, and Succinate on Salmonella Typhimurium Infections In Mice

Cited by 16 publications

References 0 publications

Blood Citric Acid Concentration as Affected by Heat and Cold Stress and Adrenocorticotrophic Hormone

Blood Citric Acid Concentration as Affected by Heat and Cold Stress and Adrenocorticotrophic Hormone

Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo

Studies on Experimental Shigellosis

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