The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke

Nakajima, Hideto; Unoda, Kiichi; Ito, Takumi; Kitaoka, Haruko; Kimura, Fumiharu; Hanafusa, Toshiaki

doi:10.2174/1874205x01206010051

Cited by 48 publications

(34 citation statements)

References 35 publications

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“…8-OhdG is one of the predominant forms of free radical-induced lesions of DNA and is excreted into the urine. The urinary 8-OhdG is considered as an important biomarker of generalized and cellular oxidative stress to DNA (Nakajima et al, 2012). In this study, the level of urinary 8-OhdG was significantly higher in H1N1 infected patients than in the control subjects.…”

Section: Discussionmentioning

confidence: 58%

Enhanced oxidative damage to DNA, lipids, and proteins and levels of some antioxidant enzymes, cytokines, and heat shock proteins in patients infected with influenza H1N1 virus

Lim¹,

Oh²,

Kim³

et al. 2014

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Summary. -Influenza virus is well known for pandemics and epidemics with high morbidity. Many studies have been focused to this problematics, however, oxidative damage to DNA, lipids, and proteins and the expression of cytokines, antioxidant enzymes and heat-shock proteins (HSPs) in H1N1 virus-infected patients has not been investigated yet. Therefore we aimed this study at these issues. We found out that patients infected with the 2011 H1N1 virus as compared to control subjects exhibited an increased oxidative damage to DNA, lipids and proteins as assayed by levels of 8-hydroxydeoxyguanosine (8-OhdG) and malondialdehyde (MDA) and carbonyl content in plasma and urine. Moreover, the levels of antioxidant enzymes SOD and catalase, cytokines Il-6, 1L-10 and TNF-α and HSPs 90 and 27 were also significantly higher in the H1N1-infected patients. Our results suggest that the influenza H1N1 virus is a strong inducer of (i) oxidative damage to DNA, lipids, and proteins, (ii) antioxidant enzymes, (iii) cytokines and (iv) HSPs.Keywords: influenza H1N1 virus; patients; oxidative damage; antioxidant enzymes; cytokines; heat-shock proteins * Corresponding authors. E-mail: dsul@korea.ac.kr, litjacob@chol. com; phone: +82-2-920-6614. # These authors contributed equally to this work. Abbreviations: 8-OhdG = 8-hydroxydeoxyguanosine; SOD = superoxide dismutase; IL = interleukin; TNF-α = tumor necrosis factor alpha; MDA = malondialdehyde; Prx = peroxiredoxin; HSPs = heat shock proteins; ROS = reactive oxygen species

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Section: Discussionmentioning

confidence: 58%

Enhanced oxidative damage to DNA, lipids, and proteins and levels of some antioxidant enzymes, cytokines, and heat shock proteins in patients infected with influenza H1N1 virus

Lim¹,

Oh²,

Kim³

et al. 2014

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“…Kadar 8-OHdG dalam serum yang tinggi menandakan kerusakan otak yang berat dan akan tercermin pada defisit neurologis yang terjadi akibat strok iskemik. [1][2][3] Radikal bebas yang terbentuk waktu terjadi iskemia otak akan melakukan interaksi dengan basa guaninnya pada gugus C8 yang merupakan target utama. Kemudian, guanin akan teroksidasi menyebabkan terbentuknya 8-oxo-Gua (bentuk nukleotida) yang dapat menyebabkan kegagalan DNA repair.…”

Section: Pendahuluanunclassified

“…Keuntungan metode mikro ELISA itu adalah pengerjaannya yang cepat, mudah, murah, menggunakan sedikit peralatan, memberikan hasil akurat. 3 Penelitian oleh Brea dkk. 5 pada tahun 2009 di Spanyol dan Nakajima dkk.…”

Section: Pendahuluanunclassified

“…5 pada tahun 2009 di Spanyol dan Nakajima dkk. 3 pada Juli 2007− Februari 2008 di Osaka mendapatkan hasil bahwa penanda 8-OHdG merupakan penanda stres oksidatif yang dapat digunakan untuk mengetahui derajat kerusakan sel otak serta menilai luaran klinis pada penderita strok iskemik. Berdasarkan uraian tersebut, maka peneliti tertarik untuk mengetahui korelasi antara kadar 8-OHdG serum dan derajat defisit neurologis pada penderita strok iskemik di Rumah Sakit Dr. Hasan Sadikin Bandung, sehingga diharapkan dapat membantu klinisi menilai derajat kerusakan otak akibat strok iskemik, memprediksi stres oksidatif, serta mengetahui prognosis penderita strok iskemik.…”

Section: Pendahuluanunclassified

“…Penurunan hormon estrogen dapat memudahkan proses aterosklerosis dan meningkatkan risiko strok. 7,3 Risiko strok meningkat sejak usia 45 tahun baik pada laki-laki ataupun perempuan. Keadaan ini dapat terjadi karena sejalan dengan bertambahnya usia terjadi proses degenerasi yang akan meningkatkan proses aterosklerosis yang disebabkan penimbunan plak di dalam pembuluh darah, sehingga pembuluh darah akan mengalami penyempitan.…”

Section: Pembahasanunclassified

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Korelasi Kadar 8-Hydroxy-2-Deoxyguanosine (8-OHdG) Serum Dengan Derajat Defisit Neurologis pada Strok Iskemik

Liza¹,

Parwati²,

Birawa³

et al. 2014

mkb

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AbstrakPeningkatan stres oksidatif, sekresi radikal bebas pada strok iskemik dapat merusak inti sel neuron di otak. Peningkatan kadar 8-hydroxy-2-deoxyguanosine (8-OHdG) serum pada strok iskemik yang merupakan hasil interaksi radikal bebas dengan gugus C8 basa guanin menandakan kerusakan sel neuron otak yang tercermin pada penilaian defisit neurologis menggunakan skor The National Institutes of Health Stroke Scale (NIHSS). Tujuan penelitian ini untuk mengetahui korelasi kadar 8-OHdG serum dengan derajat defisit neurologis menggunakan skor NIHSS. Bentuk penelitian adalah observasional dengan desain penelitian potong lintang. Penelitian dilakukan di RSUP Dr. Hasan Sadikin Bandung pada bulan Agustus 2013 hingga Januari 2014. Subjek penderita stroke iskemik onset akut berdasarkan CT-scan. Analisis statistik menggunakan Uji Kruskal-Wallis, Uji korelasi Rank Spearman. Sebanyak 72 subjek penelitian diperoleh median kadar 8-OHdG dalam serum dengan defisit neurologis ringan 3,9 ng/mL (3,3-12,0 ng/mL), sedang 23 ng/mL (8,0-51,0 ng/mL), berat 77,5 ng/mL (54,0-97,0 ng/mL). Korelasi kadar 8-OHdG serum dengan derajat defisit neurologis r s =0,912 (p<0,001). Simpulan, kadar 8-OHdG dalam serum pada strok iskemik berkorelasi positif sangat kuat dengan derajat defisit neurologis, hal ini dapat dipertimbangkan untuk digunakan pada keadaan ketidaksesuaian antara gambaran CT-scan dan klinis atau pada fasilitas kesehatan yang tidak mempunyai CT-scan. Neurological Deficits in Ischemic Stroke AbstractIncreased oxidative stress and free radicals can cause neuron cell damage. Serum 8-hydroxy-2-deoxyguanosine (8-OHdG) concentration is the result of free radical interactions with cluster C8 guanine bases, which is used to assess the degree of neuron cells damage and the oxidative stress levels. Increased serum 8-OHdG concentration indicating brain cells damage is reflected in neurological deficits based on the The National Institutes of Health Stroke Scale (NIHSS). The aim of this study was to determine the correlation between serum 8-OHdG concentration and the degree of neurological deficit by NIHSS. This was an observational cross-sectional study. Seventy-two patients with acute ischemic stroke who visited Dr. Hasan Sadikin General Hospital Bandung during the period of August 2013 to January 2014 were enrolled. Statistical analysis was performed using Kruskal-Wallis test and rank Spearman's correlation test. The mild neurological deficit median serum 8-OHdG concentrations was 3.9 ng/mL (3.3-12.0 ng/mL), moderate was 23 ng/mL (8.0-51.0 ng/mL), and severe was 77.5 ng/mL (54.0-97.0 ng/ mL). Correlation of serum 8-OHdG concentration with neurological deficits in acute ischemic stroke r s =0.912 (p<0.001). In conclusion, serum 8-OHdG have a strong meaningful positive correlation with neurological deficits. Serum 8-OHdG concentration may be considered to be use in the assessment of discrepancy between CT scan and clinical symptoms and in health facilities with no CT scan facility. [MKB. 2014;46(3):177-82]

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A fully validated bioanalytical method using an UHPLC–MS/MS system for quantification of DNA and RNA oxidative stress biomarkers

et al. 2017

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A new, rapid and effective ultra-high-performance liquid chromatography method with mass spectrometry detection is described for the separation and quantification of 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine and creatinine in human urine. The present study uses an isotope-labelled internal standard ([N]-8-hydroxy-2-deoxyguanosine), a BIO core-shell stationary phase and an isocratic elution of methanol and water. Sample preparation of human urine was performed by solid-phase extraction (SPE) on Oasis HLB cartridges with methanol/water 50:50 (v/v) elution. Extraction recoveries ranged from 98.1% to 109.2%. Biological extracts showed high short-term stability. Several aspects of this procedure make it suitable for both clinical and research purposes: a short elution time of less than 3.2 min, an intra-day precision of 2.5-8.9%, an inter-day precision of 3.4-8.7% and low limits of quantification (27.7 nM for 8-hydroxyguanosine, 6.0 nM for 8-hydroxy-2-deoxyguanosine). Finally, simultaneous analysis of DNA and RNA oxidative stress biomarkers is a useful tool for monitoring disease progression in neurodegenerative disorders and cancer. Graphical abstract UHPLC-MS/MS analysis of DNA and RNA oxidative stress biomarkers.

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The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke

Cited by 48 publications

References 35 publications

Enhanced oxidative damage to DNA, lipids, and proteins and levels of some antioxidant enzymes, cytokines, and heat shock proteins in patients infected with influenza H1N1 virus

Enhanced oxidative damage to DNA, lipids, and proteins and levels of some antioxidant enzymes, cytokines, and heat shock proteins in patients infected with influenza H1N1 virus

Korelasi Kadar 8-Hydroxy-2-Deoxyguanosine (8-OHdG) Serum Dengan Derajat Defisit Neurologis pada Strok Iskemik

A fully validated bioanalytical method using an UHPLC–MS/MS system for quantification of DNA and RNA oxidative stress biomarkers

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