The Relationship between Chromosomal Positioning within the Nucleus and the<i>SSD1</i>Gene in<i>Saccharomyces cerevisiae</i>

Yanamoto, Toshiaki; Miyamoto, Akihiro; Ikeda, Kayoko; Hatano, Takushi; Matsuzaki, Hiroaki

doi:10.1271/bbb.110242

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…In addition, cell well integrity is vital for survival and pathogenesis but the regulatory mechanisms are complicated ( Reinke et al, 2004 ). In the present study, CrSsd1 deletion mutants displayed greater sensitivity to the cell wall inhibitor CR, consistent with previous observations for S. cerevisiae and C. albicans ( Moriya and Isono, 1999 ; Gank et al, 2008 ; Yanamoto et al, 2011 ). Ssd1 has been implicated in the maintenance of cell wall integrity in C. albicans , and deletion of Ssd1 can render cells more susceptible to cell wall-perturbing agents such as Calcofluor white ( Ram and Klis, 2006 ; Gank et al, 2008 ).…”

Section: Discussionsupporting

confidence: 93%

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Jiang

Hasan

et al. 2020

Front. Microbiol.

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Cell wall biogenesis protein phosphatases play important roles in various cellular processes in fungi. However, their functions in the widely distributed mycoparasitic fungus Clonostachys rosea remain unclear, as do their potential for controlling plant fungal diseases. Herein, the function of cell wall biogenesis protein phosphatase CrSsd1 in C. rosea 67-1 was investigated using gene disruption and complementation approaches. The gene-deficient mutant ΔCrSsd1 exhibited much lower conidiation, hyphal growth, mycoparasitic ability, and biocontrol efficacy than the wild-type (WT) strain, and it was more sensitive to sorbitol and Congo red. The results indicate that CrSsd1 is involved in fungal conidiation, osmotic stress adaptation, cell wall integrity, and mycoparasitism in C. rosea.

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Section: Discussionsupporting

confidence: 93%

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Jiang

Hasan

et al. 2020

Front. Microbiol.

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“…YPAD nutrient medium and synthetic media SGlc, SGal, and SRaf, containing glucose, galactose, and raffinose as carbon sources for S. cerevisiae, and LB medium for E. coli were described previously. 7) The yeasts were cultivated at 28 C, and E. coli at 37 C. Standard genetic methods for E. coli and S. cerevisiae were applied, as described by Sambrook et al 8) and Rose et al 9) respectively. Plasmid pHM209, used for the expression of R recombinase was described previously.…”

Section: Methodsmentioning

confidence: 99%

Cell Death Caused by Excision of Centromeric DNA from a Chromosome inSaccharomyces cerevisiae

Miyamoto

Yanamoto

Matsumoto

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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If genetically modified organisms (GMOs) are spread through the natural environment, it might affect the natural environment. To help prevent the spread of GMOs, we examined whether it is possible to introduce conditional lethality by excising centromeric DNA from a chromosome by site-specific recombination in Saccharomyces cerevisiae as model organism. First, we constructed haploid cells in which excision of the centromeric DNA from chromosome IV can occur due to recombinase induced by galactose. By this excision, cell death can occur. In diploid cells, cell death can also occur by excision from both homologous chromosomes IV. Furthermore, cell death can occur in the case of chromosome V. A small number of surviving cells appeared with excision of centromeric DNA, and the diploid showed greater viability than the haploid in both chromosomes IV and V. The surviving cells appeared mainly due to deletion of a recombination target site (RS) from the chromosome.

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Yeast Ssd1 is a non-enzymatic member of the RNase II family with an alternative RNA recognition site

Bayne

Jayachandran

Kasprowicz

et al. 2021

Nucleic Acids Research

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Ssd1, a conserved fungal RNA-binding protein, is important in stress responses, cell division and virulence. Ssd1 is closely related to Dis3L2 of the RNase II family of nucleases, but lacks catalytic activity and likely suppresses translation of bound mRNAs. Previous studies identified RNA motifs enriched in Ssd1-associated transcripts, yet the sequence requirements for Ssd1 binding are not defined. Here, we identify precise binding sites of Ssd1 on RNA using in vivo cross-linking and cDNA analysis. These sites are enriched in 5′ untranslated regions of a subset of mRNAs encoding cell wall proteins. We identified a conserved bipartite motif that binds Ssd1 with high affinity in vitro. Active RNase II enzymes have a characteristic, internal RNA binding path; the Ssd1 crystal structure at 1.9 Å resolution shows that remnants of regulatory sequences block this path. Instead, RNA binding activity has relocated to a conserved patch on the surface of the protein. Structure-guided mutations of this surface prevent Ssd1 from binding RNA in vitro and phenocopy Ssd1 deletion in vivo. These studies provide a new framework for understanding the function of a pleiotropic post-transcriptional regulator of gene expression and give insights into the evolution of regulatory and binding elements in the RNase II family.

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The Relationship between Chromosomal Positioning within the Nucleus and theSSD1Gene inSaccharomyces cerevisiae

Cited by 3 publications

References 26 publications

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Cell Death Caused by Excision of Centromeric DNA from a Chromosome inSaccharomyces cerevisiae

Yeast Ssd1 is a non-enzymatic member of the RNase II family with an alternative RNA recognition site

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