The Relationship between Circulating Levels of Osteopontin with Carotid Intima-Media Thickness in Children on Regular Hemodialysis

Mohamed, Raghda; Tawfeek, Enas; Abdel-Salam, Manal; Ghoraba, Niveen M.; Maghraby, Hala

doi:10.4236/ojneph.2021.114039

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…OPN belongs to the small integrin binding ligand N-glycosylated (SIBLINGs) group, which is a group of 5 integrins related to glycophosphoproteins and is found in odontoblasts, osteoblasts, and osteocytes (Aditiyono et al, 2018; Boskey et al, 2012) [49]. OPN plays an important role in regulating vascular classification and regulating bone remodeling (Mohamed et al, 2021) [50].…”

Section: Osteopontinmentioning

confidence: 99%

Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human umbilical cord mesenchymal stem cells

Michael Josef Kridanto Kamandjadja,

Erike Dwi Safitri,

Patricya Talitha Damayanti

et al. 2024

World J. Adv. Res. Rev.

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In recent years, regenerative therapy using tissue engineering has rapidly developed. One of the applications is for alveolar bone tissue engineering in prosthodontics. Currently, the most widely developed biomaterial in bone tissue engineering is a scaffold. The incorporation of chitosan and hydroxyapatite (CS-HA) improves osteoconductive ability. Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) are capable of acting as osteoprogenitors and promoting bone formation. Purpose: To determine the influence of osteocalcin secretion in the CS[1]HA scaffold with seeding of cryopreserved hUCMSCs in vitro. Methods: The thawing process was carried out on frozen hUCMSCs, which were then examined for characterization using immunocytochemistry. The hUCMSCs were seeded on the CS-HA scaffold. Finally, examination of osteocalcin secretion levels was carried out by collecting cell culture supernatant on the 14th and 21st days of observation groups using the human osteocalcin ELISA kit. Results: Immunocytochemistry testing revealed that the hUCMSCs had the characteristics of the MSCs marker, namely positive expression on the CD90 marker and negative expression on the CD45 marker. Scanning electron microscope (SEM) observation revealed that the CS-HA scaffold had a structure with many pores and hUCMSCs could be attached and proliferation among the porosity of the scaffold. Based on the ELISA result, there was a significant difference in the average amount of osteocalcin secretion on days 14 and 21 which indicated osteogenic differentiation. Conclusion: Osteocalcin secretion in the CS-HA scaffold using the seeding of in vitro hUCMSCs demonstrated a bone regeneration process that showed a significant increase on day 21.

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Section: Osteopontinmentioning

confidence: 99%

Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human umbilical cord mesenchymal stem cells

Michael Josef Kridanto Kamandjadja,

Erike Dwi Safitri,

Patricya Talitha Damayanti

et al. 2024

World J. Adv. Res. Rev.

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Osteopontin and Clinical Outcomes in Hemodialysis Patients

Torino,

Carbone,

Pizzini

et al. 2024

Biomedicines

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Background/Objectives: Chronic kidney disease (CKD) and end-stage kidney disease (ESKD) are significant public health issues, with cardiovascular morbidity and mortality being the leading causes of death in hemodialysis patients. Osteopontin (OPN), a multifunctional glycoprotein, has emerged as a potential biomarker for vascular disease in CKD due to its role in inflammation, tissue remodeling, and calcification. Methods: This cohort study included 1124 hemodialysis patients from the PROGREDIRE study, a registry involving 35 dialysis units in Southern Italy. Serum osteopontin levels were measured using enzyme-linked immunosorbent assay (ELISA). The primary endpoints were all-cause and cardiovascular mortality. Multivariate Cox regression analyses were performed to assess the association between osteopontin levels and mortality, adjusting for traditional risk factors, biomarkers of inflammation, nutritional status, and ESKD-related factors. Results: During a mean follow-up of 2.8 years, 478 patients died, 271 from cardiovascular causes. Independent correlates of osteopontin included alkaline phosphatase and parathyroid hormone. Elevated osteopontin levels were significantly associated with increased all-cause mortality (HR 1.19, 95% CI 1.09–1.31, p < 0.001) and cardiovascular mortality (HR 1.22, 95% CI 1.08–1.38, p = 0.001) after adjusting for confounders. Conclusions: Elevated osteopontin levels are associated with increased all-cause and cardiovascular mortality in hemodialysis patients. These findings implicate osteopontin in the high risk for death and cardiovascular disease in the hemodialysis population. Intervention studies are needed to definitively test this hypothesis.

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Serum osteopontin level is independently associated with arterial stiffness in patients on hemodialysis

Huang,

Hsu,

Wang

et al. 2024

Tzu Chi Medical Journal

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Objectives: Carotid–femoral pulse wave velocity (cfPWV) is an approach primarily adopted to define arterial stiffness (AS), which is one of the major contributors to unfavorable cardiovascular outcomes. Osteopontin (OPN), in addition to regulation of bone homeostasis, is an inflammatory mediator of atherosclerosis. We performed a research which estimated the correlation between blood OPN levels and AS in participants on maintenance hemodialysis (MHD). Materials and Methods: One hundred and twenty-six patients who received long-term MHD were included in the cross-sectional study. cfPWV values were calculated based on the carotid and femoral pulsation waveforms. Patients with cfPWV >10 m/s were categorized into the AS group. We utilized a commercially available enzyme-linked immunosorbent assay to check serum concentrations of OPN. Results: Study patients belonging to AS were found to be older, had significantly higher prevalence of underlying diabetes mellitus (DM) and hypertension, had higher systolic blood pressure, and had higher serum total calcium and OPN levels. After adjusting for these variables, multivariate logistic regression analysis disclosed that OPN levels, older age, DM, and total serum calcium levels were independently correlated with AS in patients on MHD. Multivariate analysis based on forward stepwise linear regression also showed that the logarithmically transformed OPN level was an independent correlate of cfPWV in these participants. Conclusion: Serum OPN concentrations had a positive correlation with cfPWV and were therefore related to AS in patients on MHD.

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The Relationship between Circulating Levels of Osteopontin with Carotid Intima-Media Thickness in Children on Regular Hemodialysis

Cited by 3 publications

References 38 publications

Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human umbilical cord mesenchymal stem cells

Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human umbilical cord mesenchymal stem cells

Osteopontin and Clinical Outcomes in Hemodialysis Patients

Serum osteopontin level is independently associated with arterial stiffness in patients on hemodialysis

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