Objective: This paper aims to explore the investigation, analysis, and countermeasures of the psychological pressure of college teachers. Method: From March 2023 to May 2023, 3266 college teachers in our city were selected as the research objects, and the sources of their psychological stress were analyzed through a questionnaire survey. Results: In this paper, there were 500 cases of education and teaching pressure accounting for 15.31%, 600 cases of research pressure accounting for 18.37%, 1000 cases of professional title evaluation pressure accounting for 30.62%, and 1266 cases of life pressure accounting for 35.70%. Conclusion: The psychological pressure on teachers in colleges and universities in our city is relatively high, and this paper puts forward targeted countermeasures for this problem.