The relationship between conidiation and alkaloid production in saprophytic strains of <i>Claviceps purpurea</i>

Pažoutová, Sylvie; Pokorný, Vladimír; ↠eháček, Z.

doi:10.1139/m77-178

Cited by 20 publications

(7 citation statements)

References 3 publications

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“…Several papers have shown that Aspergillus mutants deficient in sporulation are also unable to produce aflatoxin (see reference 12 and references therein). In 1977, it was shown that Claviceps purpurea mutants lacking the ability to form conidia on agar plates also produce less toxic alkaloids (93). In the same year, Sekiguchi and Gaucher (103) reported that P. urticae NRRL 2159A mutants blocked at an early stage of conidiation produced markedly less patulin under growth conditions which normally supported patulin biosynthesis.…”

Section: Mycotoxinsmentioning

confidence: 99%

Relationship between Secondary Metabolism and Fungal Development

Calvo

Wilson

Bok

et al. 2002

Microbiol Mol Biol Rev

944

652

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Filamentous fungi are unique organisms—rivaled only by actinomycetes and plants—in producing a wide range of natural products called secondary metabolites. These compounds are very diverse in structure and perform functions that are not always known. However, most secondary metabolites are produced after the fungus has completed its initial growth phase and is beginning a stage of development represented by the formation of spores. In this review, we describe secondary metabolites produced by fungi that act as sporogenic factors to influence fungal development, are required for spore viability, or are produced at a time in the life cycle that coincides with development. We describe environmental and genetic factors that can influence the production of secondary metabolites. In the case of the filamentous fungus Aspergillus nidulans, we review the only described work that genetically links the sporulation of this fungus to the production of the mycotoxin sterigmatocystin through a shared G-protein signaling pathway

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Section: Mycotoxinsmentioning

confidence: 99%

Relationship between Secondary Metabolism and Fungal Development

Calvo

Wilson

Bok

et al. 2002

Microbiol Mol Biol Rev

944

652

View full text Add to dashboard Cite

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“…Obviously one is C. purpurea strain 129 described by PA2OUTOVA et al [19], which produces 4,5 g/l clavines predominantly agroclavine (76 %). One striking feature of this particular strain is the formation of micro-and macroconidia.…”

Section: Discussionmentioning

confidence: 99%

A New Strain of Claviceps purpurea Accumulating Tetracyclic Clavine Alkaloids

Schumann

Erge²,

Maier³

et al. 1982

Planta Med

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A new strain of Claviceps was isolated from a blokked mutant of Claviceps purpurea. This strain accumulates substantial amounts of clavine alkaloids (2 g/l). The alkaloid fraction is composed of chanoclavine-I ( approximately 10%) and a mixture of agroclavine/elymoclavine (90%). Most suitable for alkaloid production in submerged culture is an ammoncitrate/sucrose medium. The genealogy of the new strain, designated Pepty 695/ch-I is the following one: Pepty 695/S (ergotoxine producer) --> Pepty 695/ch (secoergoline producer) --> Pepty 695/ch-I (tetracyclic clavine producer).

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“…First, the stimulant may stimulate a physiological change in fungal cells with tentoxin as a product or a by-product of that physiological activity. For example, Pazoutova et al (21) reported a direct relationship between alkaloid synthesis and conidia formation in a series of mutants of the fungus Claviceps purpurea 129. This is similar to the result of another study in which we found that the addition of aged culture filtrate to cultures of A. alternata would also increase the sporulation of the fungus (unpublished data).…”

Section: Appl Environ Microbiolmentioning

confidence: 99%

Stimulation of Tentoxin Synthesis by Aged-Culture Filtrates and Continued Synthesis in the Presence of Protein Inhibitors

Sheu

Talburt

1986

Appl Environ Microbiol

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Tentoxin, a cyclic tetrapeptide produced by Alternaria alternata (Fries) Keissler, induces chlorosis in certain seedling plants. It can be extracted from culture filtrates of the fungus. Tentoxin production is stimulated and increased by using a mixture of aged culture filtrates and modified Richards solution. Aged culture filtrates can be obtained from 3-week-old or older cultures of A. alternata in modified Richards solution or Pratts solution. A mixture of aged culture filtrate and fresh medium in the ratio 2:3 gives the maximal enhancement of tentoxin production. This growth system provided us with a model for studying the effects of protein synthesis inhibitors on tentoxin production. Two antibiotics which inhibit protein synthesis at the ribosomal level were tested on growth, protein synthesis, and tentoxin production in A. alternata cultures. Cycloheximide at concentrations of 500 ,ug/ml or emetine at concentrations of 250 ,ug/ml did not inhibit tentoxin synthesis, although they stopped mycelial growth and protein synthesis of the fungus at the logarithmic growth stage in the enhancement medium. These results led us to conclude that tentoxin, like certain other bioactive cyclic peptides, is synthesized by a nonribosomal peptide synthesis mechanism.

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The relationship between conidiation and alkaloid production in saprophytic strains of Claviceps purpurea

Cited by 20 publications

References 3 publications

Relationship between Secondary Metabolism and Fungal Development

Relationship between Secondary Metabolism and Fungal Development

A New Strain of Claviceps purpurea Accumulating Tetracyclic Clavine Alkaloids

Stimulation of Tentoxin Synthesis by Aged-Culture Filtrates and Continued Synthesis in the Presence of Protein Inhibitors

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