The relationship between induced reciprocal translocations and cell killing of rhesus monkey spermatogonial stem cells after combined treatments with follicle-stimulating hormone and X-rays

Buul, Paul P.W. van; Seelen, Maud C. J.

doi:10.1016/0165-7992(91)90026-z

Cited by 5 publications

(5 citation statements)

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“…Similar observations have been made for the mouse [61]. As discussed before [18], the low recovery of radiation-induced reciprocal translocations in rhesus monkey stem cell spermatogonia itself is mainly due to postirradiation proliferation-differentiation patterns of surviving spermatogonia. That no induction was found at all in the present study is probably due to a combination of two other factors.…”

Section: Discussionsupporting

confidence: 66%

“…The earlier data obtained on prepubertally irradiated monkeys were rather limited (i.e., seven individuals in total [18,59,60]), but the present, more extensive results point in the same direction: that no clear differences in chromosomal radiosensitivity exist between monkeys irradiated before or after the onset of puberty. Similar observations have been made for the mouse [61].…”

Section: Discussionmentioning

confidence: 38%

“…The first is the relatively high doses of irradiation used, which kill all stem cells sensitive to translocation induction and leave the resistant ones. The existence of sensitive and resistant stem cells leads to a socalled humped dose-response relationship, which has been observed in all mammals studied so far [26], and, in the rhesus monkey, to no induction at all in the higher dose range [18]. A second factor might be the long recovery periods after irradiation in the present study, causing the elimination of stem cell clones carrying aberrations [62].…”

Section: Discussionmentioning

confidence: 89%

“…Furthermore, spermatogenesis in both the human and the monkey has been shown to be rather radiosensitive [3,17]. The induction of genetic damage, such as stable chromosomal aberrations, seems to be very low in the rhesus monkey, probably due to the extensive spermatogonial stem cell killing by the irradiation [18].…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Effects of Irradiation Before Adulthood on Reproductive Function in the Male Rhesus Monkey1

Rooij

Kant

Dol

et al. 2002

Biology of Reproduction

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Today, many patients, who are often young, undergo total body irradiation (TBI) followed by bone marrow transplantation. This procedure can have serious consequences for fertility, but the long-term intratesticular effects of this treatment in primates have not yet been studied. Testes and epididymides of rhesus monkeys that received doses of 4-8.5 Gy of TBI at 2-4 yr of age were studied 3-8 yr after irradiation. In all irradiated monkeys, at least some seminiferous tubule cross-sections lacked germ cells, indicating extensive stem cell killing that was not completely repaired by enhanced stem cell renewal, even after many years. Testes totally devoid of germ cells were only found in monkeys receiving doses of 8 Gy or higher and in both monkeys that received two fractions of 6 Gy each. By correlating the percentage of repopulated tubules (repopulation index) with testicular weight, it could be deduced that considerable numbers of proliferating immature Sertoli cells were killed by the irradiation. Because of their finite period of proliferation, Sertoli cell numbers did not recover, and potential adult testis size decreased from approximately 23 to 13 g. Most testes showed some dilated seminiferous tubules, indicating obstructed flow of the tubular fluid at some time after irradiation. Also, in 8 of the 29 irradiated monkeys, aberrant, densely packed Sertoli cells were found. The irradiation did not induce stable chromosomal translocations in spermatogonial stem cells. No apparent changes were seen in the epididymides of the irradiated monkeys, and the size of the epididymis adjusted itself to the size of the testis. In the irradiated monkeys, testosterone and estradiol levels were normal, whereas FSH levels were higher and inhibin levels lower when testicular weight and spermatogenic repopulation were low. It is concluded that irradiation before adulthood has considerable long-term effects on the testis. Potential testis size is reduced, repopulation of the seminiferous epithelium is generally not complete, and aberrant Sertoli cells and dilated tubules are formed. The latter two phenomena may have further consequences at still longer intervals after irradiation.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 38%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Long-Term Effects of Irradiation Before Adulthood on Reproductive Function in the Male Rhesus Monkey1

Rooij

Kant

Dol

et al. 2002

Biology of Reproduction

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“…On the other hand, all results so far reported on high exposure indicate some induction of translocation (van Buul, 1991). Leenhouts and Chadwick (1981) and van Buul and Seelen (1991) have postulated three main parameters of the stem cell spermatogonial population that affect the level of induced translocations: 1) the proportion of sensitive and resistant cells; 2) the ratio of the probabilities that a basic lesion in the DNA leads to cell killing or translocation formation, and 3) the proliferation-differentiation pattern of surviving spermatogonial stem cells after irradiation. Significant increase of intra-as well as inter-in- Tables I and II. dividual variability is produced by high degrees of cell killing due to the increase in the effect of clonal proliferation of the relatively few surviving stem cells (Léonard and Deknudt, 1969;van Buul and Léonard, 1984).…”

Section: Discussionmentioning

confidence: 95%

A multivariate approach to the association pattern of reciprocal translocations induced by chemicals and ionizing radiation in mouse germ cells

2000

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The degree of similarity between chemical and physical agents in their capacity to induce reciprocal translocations was analyzed by means of multivariate analysis techniques. The effect of three different doses of gamma rays, four doses of X-rays and different doses of adriamycin, mitomycin C, thio-tepa and bleomycin was analyzed. Data were arranged in a basic matrix by two methods: cluster analysis and ordination. Two main groups were found, one including doses of 9 and 10 Gy and the other including the remaining lower doses of ionizing radiation and the other chemicals. Various subgroups were found within the second group. Accordingly, using presence/absence data there was not a specific pattern of chromosomal damage induction for physical and chemical agents. The increase in the frequencies of reciprocal translocation observed with 9 and 10 Gy was due to an increase in the kind of multivalent configurations. This variability could be dose dependent. Likewise, the similarity observed in the second group between the chemicals and the lower doses of ionizing radiation could also be dose dependent.

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X-ray induced translocations in premeiotic germ cells of monkeys

Buul

1991

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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The relationship between induced reciprocal translocations and cell killing of rhesus monkey spermatogonial stem cells after combined treatments with follicle-stimulating hormone and X-rays

Cited by 5 publications

References 36 publications

Long-Term Effects of Irradiation Before Adulthood on Reproductive Function in the Male Rhesus Monkey1

Long-Term Effects of Irradiation Before Adulthood on Reproductive Function in the Male Rhesus Monkey1

A multivariate approach to the association pattern of reciprocal translocations induced by chemicals and ionizing radiation in mouse germ cells

X-ray induced translocations in premeiotic germ cells of monkeys

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