The relationship between insecticidal effects and chitinase activities of Coleopteran-originated entomopathogens and their chitinolytic profile

Bahar, Ali Adem; Sezen, Kazım; Demirbağ, Zihni; Nalçacıoğlu, Remziye

doi:10.1007/s13213-011-0301-y

Cited by 31 publications

(21 citation statements)

References 27 publications

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“…Research focused on identifying isolates producing chitinase adds to the benefit of recognition of strains with high insecticidal activity. For example, Bahar et al (2011) identified chitinase-positive bacteria such as Serratia marcescens to be active in killing the coleopteran insects with more chitin in their exoskeleton.…”

Section: Other Bacteria With Insecticidal Propertiesmentioning

confidence: 99%

Microbes as interesting source of novel insecticides: A review

Kumari¹,

Vijayabharathi²,

Srinivas³

et al. 2014

Afr. J. Biotechnol.

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Microbes are ubiquitous, survive in all sorts of environments and have a profound influence on the earth. In the present day plant protection scenario, development of resistance to chemical pesticides is the major hurdle in insect pest management. In recent years, several microbes with potential insecticidal properties have come to light. Viruses, bacteria, fungi and protozoa that are known to produce an array of metabolites or toxins, form the basis for microbial insecticides. Since these versatile organisms are amenable for genetic engineering, strains with good insecticidal properties can be identified, evaluated and utilized for pest control. This paper reviews the insecticidal properties of microbes and their potential utility in pest management.

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Section: Other Bacteria With Insecticidal Propertiesmentioning

confidence: 99%

Microbes as interesting source of novel insecticides: A review

Kumari¹,

Vijayabharathi²,

Srinivas³

et al. 2014

Afr. J. Biotechnol.

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“…For instance, several baculoviruses and entomopathogenic enteric bacteria encode metalloproteases, so-called enhancins, able to degrade PM components leading to increased PM permeability, thereby allowing the pathogen to attack the underlying epithelium (Derksen and Granados, 1988;Wang and Granados, 1997;Peng et al, 1999;Hajaij-Ellouze et al, 2006). Likewise, chitinases secreted by bacterial entomopathogens disrupt the PM and are potent virulence factors because they facilitate passage of the bacteria or of bacterial toxins through the PM and subsequent attack of the epithelium (Sampson and Gooday, 1998;Rogers et al, 2008;Bahar et al, 2012). In honey bee larvae, it was shown that the PM is present from the very beginning of larval development (the youngest larvae were analysed at 8 h after egg hatching) and that the thickness of the matrix increases with age (Davidson, 1970), which should provide an increasingly effective barrier against pathogens.…”

Section: Introductionmentioning

confidence: 99%

Honey bee larval peritrophic matrix degradation during infection with Paenibacillus larvae, the aetiological agent of American foulbrood of honey bees, is a key step in pathogenesis

Garcia‐Gonzalez

Genersch

2013

Environmental Microbiology

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Paenibacillus larvae, the aetiological agent of American foulbrood (AFB) of honey bees, causes a fatal intestinal infection in larvae and invades the haemocoel by breaching the midgut. The peritrophic matrix lining the midgut epithelium in insects constitutes an effective barrier against abrasive food particles, xenobiotics, toxins and pathogens. Pathogens like P. larvae entering the host through the gut first need to overcome this barrier. To better understand AFB pathogenesis, we analysed the fate of the peritrophic matrix in honey bee larvae during P. larvae infection. Using histochemical techniques, we first established that chitin is a major component of the honey bee larval peritrophic matrix. Rearing larvae on a diet containing a fluorochrome blocking formation of the peritrophic matrix or a bacterial endochitinase revealed that a fully formed peritrophic matrix is essential for larval survival. Larvae infected by P. larvae showed total degradation of the peritrophic matrix enabling the bacteria to directly attack the epithelial cells. Carbon source utilization tests confirmed that P. larvae is able to metabolize colloidal chitin. We propose that P. larvae degrades the peritrophic matrix to allow direct access of the bacteria or of bacterial toxins to the epithelium to prepare the breakthrough of the epithelial layer.

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“…It produced quite a large chitinolytic zone with a diameter of 12.4 mm. Chitinolytic zones of insect originated bacteria had been studied earlier by Bahar et al (2012). They studied 23 bacteria and found that just 2 of them produced chitinolytic zones around 12 mm, while the others produced smaller zones, between 2 and 10 mm.…”

Section: Discussionmentioning

confidence: 99%

“…All of these chitinases retained 60% activity at alkali pH values between 7 and 11, although the highest activity was observed at pHs 8, 9, and 8.5 for ChiA, ChiB, and ChiC, respectively. The optimum pH values for the chitinases of S. marcescens were reported at 5.5 (Gal et al, 1998), 6.2 (Nawani andKapadnis, 2001), 5-6 (Brurberg et al, 1996), 5-7.5 (Lan et al, 2006), and 4-10 (Suzuki et al, 2002) for ChiA; 4-10 ( Suzuki et al, 2002;Bahar et al, 2012) and 5-6 (Brurberg et al, 1996) for ChiB; and 3-9 (Synstad et al, 2008) and4-10 (Suzuki et al, 2002) for ChiC. Researchers also reported bimodal pH distributions at around pH 4.0 and 8.0-9.0 (Okay et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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Cloning and expression of chitinase A, B, and C (chiA, chiB, chiC) genes from Serratia marcescens originating from Helicoverpa armigera and determining their activities

Danismazoglu¹,

Demir²,

Sezen³

et al. 2015

Turk J Biol

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IntroductionChitin, a linear polymer of β-1,4-linked N-acetyl-Dglucosamine (GlcNAc), is one of the most abundant biopolymers in nature. It is the main component of the exoskeleton of many invertebrates such as crustaceans, insects, and spiders and a structural component of the cell walls of most fungi and algae. In insects it functions as scaffold material, supporting the cuticles of the epidermis and trachea as well as the peritrophic membrane (PM) lining the gut epithelium (Merzendorfer and Zimoch, 2003;Ruiz-Sanchez et al., 2005;Kuzu, 2008).Chitinases are glycosyl hydrolases that catalyze the hydrolytic degradation of chitin. Chitin, which is found in the structure of cuticles and peritrophic membranes, is degraded by the chitinase enzymes. The degradation decreases the feeding and defenses of the insect and weakens it. Therefore, chitinases are important enzymes that have potential for being used as biological control agents against harmful insects (Cohen, 1987;Debaditya et al., 2007;Kuzu, 2008). Chitinases have been detected in a great variety of organisms, including those that contain chitin, such as insects, crustaceans, yeasts, and fungi, and also organisms that do not contain chitin, such as bacteria, higher plants, and vertebrates. Several genera of bacteria, including Serratia (

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The relationship between insecticidal effects and chitinase activities of Coleopteran-originated entomopathogens and their chitinolytic profile

Cited by 31 publications

References 27 publications

Microbes as interesting source of novel insecticides: A review

Microbes as interesting source of novel insecticides: A review

Honey bee larval peritrophic matrix degradation during infection with Paenibacillus larvae, the aetiological agent of American foulbrood of honey bees, is a key step in pathogenesis

Cloning and expression of chitinase A, B, and C (chiA, chiB, chiC) genes from Serratia marcescens originating from Helicoverpa armigera and determining their activities

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