The relationship between metal toxicity and cellular redox imbalance

Sharma, Sumita; Dietz, Karl‐Josef

doi:10.1016/j.tplants.2008.10.007

Cited by 968 publications

(520 citation statements)

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“…Thus, it seems that copper stress can operate in more ways than one with regard to the mechanism of enzymatic inhibition, although metal ions can bind with important functional groups, since they have a high affinity for O-, or N-or S-ligands of the enzyme proteins (Van Assche and Clijsters 1990) and may cause proteases inactivation . Effectively, heavy metals can induce cellular redox imbalance leading to oxidative stress (Sharma and Dietz 2009). The generation of reactive oxygen species is often considered to reflect a rather indirect metal effect of cellular disregulation; notably, the oxidation of side chains of amino acid residues and the formation of protein-protein covalent crosslinkage that inactivate or denaturate the protein function (Stadtman 1993;Davies 2001).…”

Section: Effect Of Copper Stress On Global Protease Activitymentioning

confidence: 99%

Proteolytic activities in Phaseolus vulgaris cotyledons under copper stress

Karmous

Khadija

Chaoui

et al. 2012

Physiol Mol Biol Plants

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The changes in the protease activities of bean cotyledons were investigated in response to copper stress. Assays using synthetic substrates and specific protease inhibitors followed by activity measurements and electrophoresis analysis allowed to study the classes of enzymes involved in the storage protein mobilization during the germination of bean (Phaseolus vulgaris L) seeds, and then identify which ones were affected in the presence of 200 μM CuCl 2 in the imbibition medium. Copper treatment affected embryo growth and total protease activity. The results of SDS-gelatin-PAGE show that Cu excess led to a decrease in protease activity of 45 to 66 kDa. Moreover, cysteine-, aspartic-and metallo-protease activities were markedly lowered under copper stress, while serine-protease one was enhanced as well as its activity dependent abundance in comparison with control. However, the relative distribution of major cysteine protease in H 2 O-germinated seeds was significantly diminished after Cu exposure. Thus, copper excess can disturb the nitrogen freeing from reserve tissues at enzymatic level; differential responses of protease classes are discussed, notably, cysteine protease in the way of storage protein mobilization and serine protease in protective mechanism one.

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Section: Effect Of Copper Stress On Global Protease Activitymentioning

confidence: 99%

Proteolytic activities in Phaseolus vulgaris cotyledons under copper stress

Karmous

Khadija

Chaoui

et al. 2012

Physiol Mol Biol Plants

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“…Indeed, photosynthesis is the most heavy metal-sensitive process [1] since excess Cu can, inter alia, affect photosynthetic electron transport on the reducing side of PSI at the level of ferredoxin. In addition, it can alter the PSII on the oxidising side by inhibiting the electron transport at P680 as well as by inactivating some PSII reaction centres [41]. A few studies indicate that excess Cu can impair the PSII electron transport on its reducing side by affecting the rate of electron transfer from tyrosine (Y Z ) to the oxidized primary donor P680 + [16].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it can alter the PSII on the oxidising side by inhibiting the electron transport at P680 as well as by inactivating some PSII reaction centres [41]. A few studies indicate that excess Cu can impair the PSII electron transport on its reducing side by affecting the rate of electron transfer from tyrosine (Y Z ) to the oxidized primary donor P680 + [16].…”

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confidence: 99%

Comparing the effects of excess copper in the leaves ofBrassica juncea(L. Czern) andBrassica napus(L.) seedlings: Growth inhibition, oxidative stress and photosynthetic damage

Feigl¹,

Kumar²,

Lehotai³

et al. 2015

Acta Biologica Hungarica

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Hydroponic experiments were conducted to compare the effects of excess copper (Cu) on growth and photosynthesis in young Indian mustard (Brassica juncea) and oilseed rape (Brassica napus). We compared the effects of excess Cu on the two Brassica species at different physiological levels from antioxidant levels to photosynthetic activity. Nine-day-old plants were treated with Cu (10, 25 and 50 µM CuSO 4 ) for 7 and 14 days. Both species took up Cu from the external solution to a similar degree but showed slight root-to-shoot translocation. Furthermore, after seven days of treatment, excess Cu significantly decreased other microelement content, such as iron (Fe) and manganese (Mn), especially in the shoots of B. napus. As a consequence, the leaves of young Brassica napus plants showed decreased concentrations of photosynthetic pigments and more intense growth inhibition; however, accumulation of highly reactive oxygen species (hROS) were not detected. After 14 days of Cu exposure the reduction of Fe and Mn contents and shoot growth proved to be comparable in the two species. Moreover, a significant Cu-induced hROS accumulation was observed in both Brassica species. The diminution in pigment contents and photosynthetic efficiency were more pronounced in B. napus during prolonged Cu exposure. Based on all the parameters, B. juncea appears to be more resistant to excess Cu than B. napus, rendering it a species with higher potential for phytoremediation.Keywords: Brassica juncea -Brassica napus -copper -oxidative stress -phytoremediation -photosynthesis INTRODUCTIONAmong heavy metals, copper in trace amounts is essential for plant life but it becomes toxic at higher concentrations. In excess, this heavy metal seriously inhibits leaf expansion at the early growth stage and it causes changes in physiological processes such as transpiration, photosynthetic electron transport and biosynthesis of chlorophyll [30]. Indeed, photosynthesis is the most heavy metal-sensitive process [1] since * Corresponding author; e-mail address: fglgbr@gmail.com + These authors contributed equally to this work. 206Gábor FeiGl et al. Biologica Hungarica 66, 2015 excess Cu can, inter alia, affect photosynthetic electron transport on the reducing side of PSI at the level of ferredoxin. In addition, it can alter the PSII on the oxidising side by inhibiting the electron transport at P680 as well as by inactivating some PSII reaction centres [41]. A few studies indicate that excess Cu can impair the PSII electron transport on its reducing side by affecting the rate of electron transfer from tyrosine (Y Z ) to the oxidized primary donor P680 + [16]. ActaAt the molecular level, excess Cu is able to induce the formation of reactive oxygen species (ROS) via the Fenton or Haber-Weiss reactions which subsequently damage proteins, nucleic acids and lipids [14]. This effect of excess Cu was supported by the positive correlation between Cu-treatment and the production of hydroxyl radicals in Arabidopsis [9]. Besides, excess Cu can indirectly cause oxida...

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“…Other symptoms observed in Ni 2+ -treated plants are related to oxidative stress (Gajewska et al 2006, Sharma andDietz 2009). When generation of reactive oxygen species (ROS) such as superoxide and peroxide exceeds their removal, injuries may occur in the cells (Agrawal et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Impact of nickel on grapevine (Vitis vinifera L.) root plasma membrane, ROS generation, and cell viability

Pavlovkin

Fiala

Čiamporová

et al. 2016

Acta Botanica Croatica

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-The present study investigated the impact of nickel (Ni 2+ ) on trans-membrane electrical potential (E M ) and permeability properties of plasma membrane (PM) in epidermal cells of adventitious grapevine roots. The relationship between disturbances of membrane functionality and the production of superoxide anion, hydrogen peroxide and cell viability after the exposure of roots to Ni 2+ was also studied. Treatments with 0.1-5 mmol L -1 NiCl 2 induced a concentration-dependent transient PM depolarization, which was recovered to the initial resting potential within 50-70 min in the presence of Ni 2+ . Longer (up to 24 h) exposure of roots to 1 mmol L -1 of Ni 2+ hyperpolarized the E M by approximately 17 mV. Application of the highest 5 mmol L -1 concentration of Ni 2+ during longer treatments (up to 48 h) resulted in the increase of membrane permeability; however the E M , cell viability, and superoxide content remained unaffected. The increase in the formation of hydrogen peroxide was time-and concentration-dependent and maximum production was recorded after 180 min of Ni 2+ treatment. We can conclude that oxidative stress resulting from an imbalance in the generation and/ or removal of hydrogen peroxide in the root tissues of grapevine was the major cause of Ni 2+ toxicity.

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The relationship between metal toxicity and cellular redox imbalance

Cited by 968 publications

References 84 publications

Proteolytic activities in Phaseolus vulgaris cotyledons under copper stress

Proteolytic activities in Phaseolus vulgaris cotyledons under copper stress

Comparing the effects of excess copper in the leaves ofBrassica juncea(L. Czern) andBrassica napus(L.) seedlings: Growth inhibition, oxidative stress and photosynthetic damage

Impact of nickel on grapevine (Vitis vinifera L.) root plasma membrane, ROS generation, and cell viability

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