The relationship between molecular content of mesenchymal stem cells derived exosomes and their potentials: Opening the way for exosomes based therapeutics

Jafari, Davod; Malih, Sara; Eslami, Seyed Sadegh; Jafari, Rasool; Darzi, Leila; Tarighi, Parastoo; Samadikuchaksaraei, Alí

doi:10.1016/j.biochi.2019.07.009

Cited by 38 publications

(24 citation statements)

References 128 publications

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“…Exosomal biomarkers possess multiple advantages compared to cell-free biomarkers. Cell-free biomarkers continuously oppose to blood degrading enzymes while exosomal biomarkers are encapsulated inside the vesicles and are more stable outside of the body after isolation so that they could be reliable than cell-free biomarkers like RNAs, that are most prone to degradation [13].…”

Section: Discussionmentioning

confidence: 99%

“…Other methods including, affinity chromatography method [57] and specially the exosome isolation kits are mostly used for exosome isolation [58]. As discussed in detail in our previous review [13], exosomes contents are heterogeneous and dependent on the methods of isolation and purification [59]. In our included studies three methods of exosome isolation (UC, Kit and SEC) and mostly UC and exoquic were used.…”

Section: Discussionmentioning

confidence: 99%

“…However, microvesicles are crucial in cellular communication and has been used as a biomarker source similar to exosomes, but they are not popular as exosomes [12]. However, there is a problem in terminology of exosomes and microvesicles; sometimes, they are mistakenly used interchangeably [13].…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic acuuracy of cerebrospinal fluid and serum-isolated exosomes for glioblastoma: a systematic review and meta-analysis

Jafari

Tiyuri

Rezaei

et al. 2020

Preprint

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Glioblastoma (GBM) is the most malignant glioma cancer with a high morbidity and mortality worldwide. Unfortunately, a routine method is not available for screening or preoperative early detection of GBM. However, early detection in a none-invasive or minimally invasive method could be beneficial and increase the survival rate. In this systematic review and meta-analysis, we aimed to examine the diagnostic accuracy of exosomal RNAs that were extracted from patients' CSF or serum for GBM diagnosis. We searched Web of Science, Scopus, PubMed (including Medline), Embase and ProQuest (as databases for grey literature) up to December 2019; we also performed backward and forward reference checking of included and relevant studies. Finally, included studies were assessed with QUADAS-2 checklist and their data extracted. We carried out a meta-analysis of included study, regarding to the diagnostic meta-analysis guidelines for obtaining pooled accuracy estimates. In addition, sensitivity analysis and meta-regression were also conducted. We retrived 1730 records from databases, nine of them included in systematic review and qualitative synthesis. Six studies were considered to statistical analysis and performed diagnostic meta-analysis. Our results suggested that the pooled sensitivity and specificity of exosomal biomarkers for GBM were 0.76 and 0.80, respectively. In addition, the pooled positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were 3.7, 0.30 and 12, respectively. The overall area under the curve (AUC) of exosomal biomarkers for GBM diagnosis was found to be 0.85. According to our results, the value of 0.85 for AUC, suggesting that exosomal biomarkers might serve as a high potential and noninvasive diagnostic tool for GBM. JP, Heese O, Reifenberger G et al: Long-term survival with glioblastoma multiforme. Brain : a journal of neurology 2007, 130(Pt 10):2596-2606. 3. Kim S-S, Harford JB, Pirollo KF, Chang EH: Effective treatment of glioblastoma requires crossing the blood-brain barrier and targeting tumors including cancer stem cells: the promise of nanomedicine. Biochemical and biophysical research communications 2015, 468(3):485-489. 4. targeting and microenvironment-responsive nanoparticles for gene delivery. Biomaterials 2013, 34(21):5294-5302. 5. Emery JD, Shaw K, Williams B, Mazza D, Fallon-Ferguson J, Varlow M, Trevena LJ: The role of primary care in early detection and follow-up of cancer. Nature Reviews Clinical Oncology 2014, 11(1):38-48. 6. Nass D, Rosenwald S, Meiri E, Gilad S, Tabibian-Keissar H, Schlosberg A, Kuker H, Sion-Vardy N, Tobar A, Kharenko O: MiR-92b and miR-9/9* are specifically expressed in brain primary tumors and can be used to differentiate primary from metastatic brain tumors. Brain pathology 2009, 19(3):375-383. 7. Schwartz SA, Weil RJ, Thompson RC, Shyr Y, Moore JH, Toms SA, Johnson MD, Caprioli RM: Proteomic-based prognosis of brain tumor patients using direct-tissue matrix-assisted laser desorption ionization mass spectrometry. Cance...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diagnostic acuuracy of cerebrospinal fluid and serum-isolated exosomes for glioblastoma: a systematic review and meta-analysis

Jafari

Tiyuri

Rezaei

et al. 2020

Preprint

Self Cite

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“…Additionally, thorough characterization of the molecular content of each EV subpopulation (microvesicles, exosomes, exomeres, etc.) will be necessary to support the potential therapeutic use of stem cell EVs (Jafari et al, 2019). A more comprehensive assessment relating to the production of EV therapeutics has also been published by Gimona et al (2017).…”

Section: Future Perspectivesmentioning

confidence: 99%

Use of Stem Cell Extracellular Vesicles as a “Holistic” Approach to CNS Repair

Branscome

Paul

Yin

et al. 2020

Front. Cell Dev. Biol.

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Neurodegeneration is a hallmark of many diseases and disorders of the central nervous system (CNS). High levels of neuroinflammation are often associated with irreparable damage to CNS cells due to the dysregulation of signaling cascades that are unable to restore a homeostatic balance. Due to the inherent complexity of the CNS, development of CNS-related therapeutics has met limited success. While stem cell therapy has been evaluated in the context of CNS repair, the mechanisms responsible for their functional properties have not been clearly defined. In recent years, there has been growing interest in the use of stem cell extracellular vesicles (EVs) for the treatment of various CNS pathologies as these vesicles are believed to mediate many of the functional effects associated with their donor stem cells. The potency of stem cell EVs is believed to be largely driven by their biological cargo which includes various types of RNAs, proteins, and cytokines. In this review, we describe the characteristic properties of stem cell EVs and summarize their reported neuroprotective and immunomodulatory functions. A special emphasis is placed on the identification of specific biological cargo, including proteins and non-coding RNA molecules, that have been found to be associated with stem cell EVs. Collectively, this review highlights the potential of stem cell EVs as an alternative to traditional stem cell therapy for the repair of cellular damage associated with diverse CNS pathologies.

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“…Moreover, several studies have revealed that MSC-derived exosomes as cargo carriers possess similar immune modulatory potential. Compared with MSCs, exosomes are stable, have no risk of aneuploidy and carry a lower possibility of immune rejection after therapy (Yu et al, 2014;Jafari et al, 2019). Interestingly, exosomes derived from AD-MSCs have been shown to inhibit cancer progression by promoting anti-cancer immunity (Lou et al, 2017) and by down-regulating the expression of vascular endothelial growth factor (VEGF) in mice .…”

Section: Discussionmentioning

confidence: 99%

A stem cell-based approach to the treatment of devil facial tumour disease (DFTD) in the Tasmanian devil (Sarcophilus harrisii)

Weeratunga¹

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The relationship between molecular content of mesenchymal stem cells derived exosomes and their potentials: Opening the way for exosomes based therapeutics

Cited by 38 publications

References 128 publications

Diagnostic acuuracy of cerebrospinal fluid and serum-isolated exosomes for glioblastoma: a systematic review and meta-analysis

Diagnostic acuuracy of cerebrospinal fluid and serum-isolated exosomes for glioblastoma: a systematic review and meta-analysis

Use of Stem Cell Extracellular Vesicles as a “Holistic” Approach to CNS Repair

A stem cell-based approach to the treatment of devil facial tumour disease (DFTD) in the Tasmanian devil (Sarcophilus harrisii)

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