The relationship between morphology and disaccharidase activity in ischemia- reperfusion injured intestine.

Varga, J; Tóth, Štefan; Tomečková, Vladimı́ra; Gregová, Kristína; Veselá, Jarmila

doi:10.18388/abp.2012_2103

Cited by 12 publications

(9 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In that previous study we highlighted significant alterations in the sucrase and maltase activities in the injured intestine relative to the time of reperfusion. We underlined also the importance of being able to correlate histological changes with disaccharidase activities because of the persistent discrepancy in the relation of sucrase and maltase activities vs. morphological status of the small intestines (Varga et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Trehalase as a possible marker of intestinal ischemia--reperfusion injury.

Pekárová¹,

Varga²,

Tomečková³

et al. 2013

Acta Biochim Pol

Self Cite

View full text Add to dashboard Cite

Different pathological affections of the small intestine cause corresponding morphological and functional changes. The present study was aimed to assess intestinal trehalase activities during ischemia and following reperfusion, correlate them with the pathological changes and determine whether trehalase could be used as a biochemical marker of the intestinal ischemia, ischemia - reperfusion injury. Wistar rats, randomly divided into 5 experimental groups (IR) (each n=15), were subjected to one hour mesenteric ischemia followed by 0, 1, 4, 12 and 24 hours of reperfusion. As a control group sham operated animals were used (n=15). The activity of trehalase was determined using an adapted Dahlqwist method. The range of intestinal injury was determined using histological (histopathological injury index and goblet cell quantification) and immunohistochemical (Ki67, InSitu TUNEL) methods. The highest activities of trehalase were recorded in the control group (C=4.42 ± 0.373 μmol/mg/h). The most altered intestinal histology detected in group IR1 was accompanied by the lowest trehalase activity (IR1=0.97 ± 0.209 μmol/mg/h; p < 0.001 C vs. IR1). Improved histological structure in the remaining reperfusion periods correlated with increase in trehalase activity. Almost normal mucosal histological architecture and 72% of the enzymatic activity were restored after 24 hours of reperfusion (IR24=3.20 ± 0.266 μmol/mg/h; p < 0.01 IR1 vs. IR24). The correlation between intestinal histology and trehalase activities during intestinal injury has been shown. Trehalase activity is closely associated with the status of the histological architecture of the small intestine.

show abstract

Section: Discussionmentioning

confidence: 99%

Trehalase as a possible marker of intestinal ischemia--reperfusion injury.

Pekárová¹,

Varga²,

Tomečková³

et al. 2013

Acta Biochim Pol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, the Shiga toxin (Stx) is reported to affect SI function by inhibiting the synthesis of BBM proteins as well as damaging the intestinal epithelium directly [ 36 ]. If the intestinal tissue is subjected to ischemia with blocked or decreased blood flow, a decrease in disaccharidase activities can be detected [ 37 ]. Chronic psychological stresses as well as deficiencies of iron and vitamin A are other factors that are associated with loss of the sucrase activity in the intestine [ 38 – 41 ].…”

Section: Secondary Deficiencies Of Si Activitymentioning

confidence: 99%

The multiple roles of sucrase-isomaltase in the intestinal physiology

Gericke¹,

Amiri²,

Naim³

2016

Mol Cell Pediatr

View full text Add to dashboard Cite

Osmotic diarrhea and abdominal pain in humans are oftentimes associated with carbohydrate malabsorption in the small intestine due to loss of function of microvillar disaccharidases. Disaccharidases are crucial for the digestion and the subsequent absorption of carbohydrates. This review focuses on sucrase-isomaltase as the most abundant intestinal disaccharidase and the primary or induced pathological conditions that affect its physiological function. Congenital defects are primary factors which directly influence the transport and function of sucrase-isomaltase in a healthy epithelium. Based on the mutation type and the pattern of inheritance, a mutation in the sucrase-isomaltase gene may exert a variety of symptoms ranging from mild to severe. However, structure and function of wild type sucrase-isomaltase can be also affected by secondary factors which influence its structure and function either specifically via certain inhibitors and therapeutic agents or generally as a part of intestinal pathogenesis, for example in the inflammatory responses. Diagnosis of sucrase-isomaltase deficiency and discriminating it from other gastrointestinal intolerances can be latent in the patients because of common symptoms observed in all of these cases.Here, we summarize the disorders that implicate the digestive function of sucrase-isomaltase as well as the diagnostic and therapeutic strategies utilized to restore normal intestinal function.

show abstract

“…Similarly, ligands of PPARc are unsaturated FAs, 15-deoxy-D12,14-prostaglandin J2 (15d-PGJ2) and oxidized low-density lipoprotein (LDL) components. Ligands of PPARb/d include also unsaturated and saturated FAs, prostacyclin, 4-hydroxy-2-nonenal (4-HNE), 4-hydroxydodeca-(2E,6Z)-dienal (4-HDDE) and very low-density lipoprotein (VLDL) components (15)(16)(17)(18).…”

Section: The Wide Variety Of Ligands For Pparsmentioning

confidence: 99%

The role of PPARγ‐mediated signalling in skin biology and pathology: new targets and opportunities for clinical dermatology

Ramot

Mastrofrancesco

Camera

et al. 2015

Experimental Dermatology

View full text Add to dashboard Cite

Yuval Ramot and Arianna Mastrofrancesco contributed equally to this work.Abstract: Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that modulate the expression of multiple different genes involved in the regulation of lipid, glucose and amino acid metabolism. PPARs and cognate ligands also regulate important cellular functions, including cell proliferation and differentiation, as well as inflammatory responses. This includes a role in mediating skin and pilosebaceous unit homoeostasis: PPARs appear to be essential for maintaining skin barrier permeability, inhibit keratinocyte cell growth, promote keratinocyte terminal differentiation and regulate skin inflammation. They also may have protective effects on human hair follicle (HFs) epithelial stem cells, while defects in PPARc-mediated signalling may promote the death of these stem cells and thus facilitate the development of cicatricial alopecia (lichen planopilaris). Overall, however, selected PPARc modulators appear to act as hair growth inhibitors that reduce the proliferation and promote apoptosis of hair matrix keratinocytes. The fact that commonly prescribed PPARc-modulatory drugs of the thiazolidine-2,4-dione class can exhibit a battery of adverse cutaneous effects underscores the importance of distinguishing beneficial from clinically undesired cutaneous activities of PPARc ligands and to better understand on the molecular level how PPARc-regulated cutaneous lipid metabolism and PPARcmediated signalling impact on human skin physiology and pathology. Surely, the therapeutic potential that endogenous and exogenous PPARc modulators may possess in selected skin diseases, ranging from chronic inflammatory hyperproliferative dermatoses like psoriasis and atopic dermatitis, via scarring alopecia and acne can only be harnessed if the complexities of PPARc signalling in human skin and its appendages are systematically dissected.

show abstract

The relationship between morphology and disaccharidase activity in ischemia- reperfusion injured intestine.

Cited by 12 publications

References 45 publications

Trehalase as a possible marker of intestinal ischemia--reperfusion injury.

Trehalase as a possible marker of intestinal ischemia--reperfusion injury.

The multiple roles of sucrase-isomaltase in the intestinal physiology

The role of PPARγ‐mediated signalling in skin biology and pathology: new targets and opportunities for clinical dermatology

Contact Info

Product

Resources

About