A study was made of the effect of a single generation of the root-knot nematode Metoidogyne incognita on the growth of potted French bean plants (Pliaseolus vulgaris) inoculated at different stages of plant maturity. In separate experiments, 3-, 11-and 13-day-old plants were inoculated before primary leaf expansion (BPLE), at the appearance of trifoliate leaves (TRIF) and at the flower bud (BDS) stages respectively, with 0, 2000, 4000 or 8000 second-stage juvenile nematodes and maintained in a growth chamber under controlled conditions. The photosynthetic rate of the plants inoculated at the TRIF and BDS stages decreased significantly with increasing inoculum level 7 days after inoculation. Although the respiration rate did not significantly change throughout the experimental period, the ratio of photosynthetic to respiration rate decreased significantly with increasing nematode inoculum level and duration of infection. Chlorophyll content, plant dry weight and the numbers of buds, flowers, pods and seeds were significantly lower in infected plants than in the controls; this effect increased with increasing levels of nematode inoculum for all three plant stages. The leaf area was significantly smaller only when nematode infection occurred at the BPLE stage. The plants which were youngest at the time of nematode infection produced the lowest yield; this appeared to result from the effect of nematodes on photosynthesis and related physiological processes.