The purpose of this study was to investigate the relationship among Calling, Role conflict, and Turnover intention of Republic of Korea Air force pilot. Specifically, we examined the mediating effect of Role conflict on the relationship between Calling and Turnover intention. Survey data from 211 Pilots in R.O.K. Air force bases were analyzed with Structural equation model, using AMOS 21.0. Results are as follows. First, Calling had negative relationships with Role conflict and Turnover intention. Second, Role conflict had a positive relationship with Turnover intention. Third, Role conflict partially mediated on the relationship between Calling and Turnover intention. These findings suggest that Calling may play a critical role in Turnover intention and Role conflict of R.O.K. Air force pilot. Finally, we discussed the implications of these results, limitations, and research suggestions for the future research.