The relationship between right duct lymph flow and extravascular lung water in dogs given alpha-naphthylthiourea.

Pine, Michael; Beach, P M; Cottrell, Thomas S.; Scola, Mallory R.; Turino, Gerard M.

doi:10.1172/jci108492

Cited by 21 publications

(4 citation statements)

References 36 publications

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“…A third commonly used model of lung injury is that induced by α‐naphthylthiourea (ANTU), which produces marked pulmonary oedema and pleural effusions resulting from increased permeability changes in the lung microvasculature (Latta, 1947; Pine et al 1976). Morphological studies by light and electron microscopy indicated that the capillary endothelial cell is the primary cellular target of ANTU toxicity (Cunningham & Hurley, 1972; Meyrick et al 1972).…”

Section: Discussionmentioning

confidence: 99%

Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice

Song

Fukuda

Bai

et al. 2000

The Journal of Physiology

132

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Aquaporin (AQP) water channels provide a major pathway for osmotically driven water movement across epithelial and microvascular barriers in the lung. We used mice deficient in each of the three principal lung aquaporins, AQP1, AQP4 and AQP5, to test the hypothesis that aquaporins are important in neonatal lung fluid balance, adult lung fluid clearance and formation of lung oedema after acute lung injury. Wet‐to‐dry weight ratios (W/D) in lungs from wild‐type mice decreased from 7.9 to 5.7 over the first hour after spontaneous delivery. AQP deletion did not significantly affect W/D at 45 min after birth. Alveolar fluid clearance was measured in living ventilated mice in which 0.5 ml saline containing radiolabelled albumin was instilled into the airspaces. Fluid clearance was 17.4% in 15 min and inhibited >90% by amiloride, but clearance was not affected by AQP deletion. W/D was measured in established models of acute lung injury – acid aspiration and thiourea administration. Two hours after intratracheal administration of HCl, W/D increased from 3.7 to 7.5 but was not affected by AQP deletion. Three hours after intraperitoneal infusion of thiourea, W/D increased to 5.5 and marked pleural effusions appeared, but there were no differences in wild‐type and AQP knockout mice. Hyperoxic subacute lung injury was induced by 95% oxygen. Neither mean survival (143 h) nor W/D at 65 h (5.1) were significantly affected by AQP deletion. Despite their role in osmotically driven lung water transport, aquaporins are not required for the physiological clearance of lung water in the neonatal or adult lung, or for the accumulation of extravascular lung water in the injured lung.

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Section: Discussionmentioning

confidence: 99%

Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice

Song

Fukuda

Bai

et al. 2000

The Journal of Physiology

132

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“…After development of pulmonary edema, the lymphatics may continue the normal function of conservation of plasma filtrate. In addition, they may function as conduits for removing accumulated extravascular fluid (Pine et al, 1976). We designed experiments to study the dynamics of transvascular fluid flux and lymph flow during and after development of pulmonary edema.…”

Section: The Dynamics Of the Lung Fluid Filtrationmentioning

confidence: 99%

The dynamics of the lung fluid filtration system in dogs with edema.

Gee¹,

Spath²

1980

Circulation Research

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SUMMARY We studied the relationship between pulmonary microvasculature fluid filtration pressures and lung lymph flow rate (Q L ) as filtration pressures increased to determine why lungs with functional lymphatics become edematous and, as filtration pressures decreased, to determine the effect of edema formation on lymphatic function. Edema was induced by rapid intravenous infusion of neutralized Ringer's solution in a volume equivalent to 30% of body weight in seven anesthetized dogs. Pulmonary microvascular pressure (Pmv) and (JL increased to 58 cm H2O and 40 ml/hr, respectively, during the infusion. Initially, QL increased slowly and the estimated net fluid filtration pressure (£p) increased rapidly with infusion. Later in the 30-minute infusion period, small increases in £ P produced greater changes in Q L . Over a 3-hour postinfusion period, Pmv and Q L decreased, but they remained significantly greater than baseline levels. During the postinfusion period, Q L was a linear function of Pmv and a logarithmic function of £P. Extravascular lung water content, measured postmortem, was 70% greater than normal. The relationship between Q L and £P during and after the infusion demonstrated marked hysteresis. These results suggest that extravascular fluid accumulated in the lung in part because the lymphatics responded relatively slowly to rapid increases in £ P . Furthermore, the data suggest that, although the lymphatics may not be a quantitatively important route for removal of edema fluid, the pressure-volume characteristics of the pulmonary interstitium seem to have a major influence on lymphatic function. Circ Res 46: 796-801, 1980 DURING development of pulmonary edema, lymphatic function may determine in what manner and to what degree fluid accumulates in the extravascular space of the lung. Erdmann et al. (1975) measured lung lymph flow rates in unanesthetized sheep subjected to steady state elevations in left atrial pressure. They found that each cm H 2 O increase in pulmonary microvascular pressure resulted in an increase in lymph flow rate of about 0.5 ml/hr with no apparent maximum in lymph flow rate over the pressure range studied. However, their data suggest that the rapidity of the response of the lymphatics to a change in transvascular fluid dynamics is a factor that must be considered in studying the relationship between lymph formation and the accumulation of pulmonary extravascular fluid.After development of pulmonary edema, the lymphatics may continue the normal function of conservation of plasma filtrate. In addition, they may function as conduits for removing accumulated extravascular fluid (Pine et al., 1976). We designed experiments to study the dynamics of transvascular fluid flux and lymph flow during and after development of pulmonary edema. Our results suggest that extravascular fluid accumulated in the lung in part because the lymphatic system responded relatively slowly to a rapid increase in the net fluid filtration rate. Furthermore, the pressure-volume characteristics of the pulmo...

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“…Many factors promote pulmonary lymph flow, including the elevation of the left atrial pressure, increased bronchial arterial perfusion, hemodilution, increased cardiac output, and so on. In contrast, lymph flow may decline with the elevation of lymphatic outflow pressure; the effective resistance of the extrapulmonary part of the lymphatic system was larger than the resistance of the lymphatics in the lungs, and it may limit the maximal flow of lymph from edematous lungs [56][57][58][59][60][61][62][63][64][65][66] .…”

Section: Pulmonary Lymphatic Vessels and Lymph Flowmentioning

confidence: 99%

Cardiopulmonary Dysfunction Caused by Mediastinal Lymph Flow Impairment

Cui¹

2016

Journal of Respiratory Research

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The important effect of mediastinal lymph flow has been overlooked in most textbooks for over three centuries, and recent progresses in lymphatic vascular biology provide a new way to discover the effect of mediastinal lymph flow impairment. Different from conventional view about lymph flow impairment, which merely refers to tissue edema other than specific tissue or organ injury, mediastinal lymph flow impairment can bring a fatal consequence. Mediastinal lymph flow impairment may cause cardiopulmonary dysfunction by interrupting both the cardiac contraction manner of cardiomyocytes "asynchronized" contraction and pulmonary perivascular lymphatic remodeling.

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The relationship between right duct lymph flow and extravascular lung water in dogs given alpha-naphthylthiourea.

Abstract: A B S T R A C T The relationship between right duct lymph flow and extravascular lung water was studied in 3 normal dogs and 15 dogs with pulmonary edema induced by a-naphthylthiourea (ANTU). Right Thus, in ANTU-induced pulmonary edema right duct

Cited by 21 publications

References 36 publications

Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice

Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice

The dynamics of the lung fluid filtration system in dogs with edema.

Cardiopulmonary Dysfunction Caused by Mediastinal Lymph Flow Impairment

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