The Relationship between Salivary Calcium Concentration and Differences in Bone Mineral Density Level in Female Rats

Hattori, Satoshi; Agata, Umon; Park, Jong Hoon; Iimura, Yuki; Tokuda, Shuji; Ezawa, Ikuko; Omi, Naomi

doi:10.3177/jnsv.60.152

Cited by 7 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study observed BMD of tibial diaphysis in OC group was higher than that in Sham group despite the opposite result in BMD of tibial proximal metaphysis. Similar trends have been observed in our previous studies that also used OVX rats 13 . These findings indicate that tibial diaphysis may be more sensitive than proximal metaphysis.…”

Section: Bmc Of Tibial Diaphysis (G)supporting

confidence: 91%

“…The bone strength of the femoral midshafts were evaluated by a three-point bending test (DYN-1255; IIO DENKI, Tokyo, Japan) using previously described methods (distance between the fulcrum 1 cm, plunger speed 100 mm/minute, full scale 50 kg, chart speed 120 cm/min) [ 13 ]. ‘‘Breaking force’’ refers to the loading weight (gravitational acceleration) required for bone breaking.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The effects of royal jelly protein on bone mineral density and strength in ovariectomized female rats

Hattori

Omi

2021

Phys Act Nutr

Self Cite

View full text Add to dashboard Cite

[Purpose] Sex hormones deficiency leads to dramatically bone loss in particular postmenopausal women. Royal jelly has anti-osteoporosis effect due to maintain bone volume in that condition. We hypothesized that royal jelly protein (RJP, a latent residue after extracting royal jelly) also prevents bone deficient in ovariectomized (OVX) female rats, the animal model of postmenopausal women. [Methods] Female Sprague-Dawley rats (n = 30, 6 weeks age old) were sham operated (Sham; sham operated group, n = 7), OVX control group (OC, n = 7), OVX with low RJP intake group (ORL, n = 8), and OVX with high RJP intake group (ORH, n = 8) during 8 weeks experimental periods. In the end point of this experiment, the bone samples (lumbar spine, tibia, and femur) were surgically removed under anesthesia. These bone samples were evaluated bone mineral density (BMD) and bone strength.[Results] BMD of lumbar spine in RJP intake groups (ORL, ORH) were higher than that in OC group (p < 0.05 and p < 0.01) in RJP intake volume dependent manner. BMD of tibial proximal metaphysis and diaphysis in RJP intake groups were also higher than these in OC group (p < 0.01 and p < 0.01 / p < 0.05 and p < 0.001). In addition, breaking force of femur in RJP intake groups were significantly increase compared with that in OC group (p < 0.001 respectively). [Conclusion] These findings indicate that RJP contribute to prevent sex hormone related bone abnormality

show abstract

Section: Bmc Of Tibial Diaphysis (G)supporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

The effects of royal jelly protein on bone mineral density and strength in ovariectomized female rats

Hattori

Omi

2021

Phys Act Nutr

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several previous studies report that estrogen is the most important hormone in maintaining bone mass and that a deficiency of this hormone is a major cause of bone loss associated with age in both genders (37)(38)(39). Notably, when the circulating estrogen level decreases, calcium in the bones rapidly decreases, and calcium loss occurs via an increase in its urinary excretion (40). In the current study, rat blood estradiol level was gauged using an ELISA kit.…”

Section: Discussionmentioning

confidence: 99%

Effect of icariin on fracture healing in an ovariectomized rat model of osteoporosis

Cao

Zhang

Qian

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Osteoporosis is frequently asymptomatic, presenting a significant clinical and economic burden, particularly following an osteoporosis-associated fracture. Icariin has been reported to inhibit osteoporosis in vitro, and the present study investigated whether icariin also promoted bone fracture healing in ovariectomized osteoporotic (OVX) rats in vivo. A total of 30 female rats were randomly divided into three groups (n=10 per group): i) Sham surgery; ii) OVX; and iii) OVX with icariin (OVX + ICA) groups. At 3 months after the ovariectomy, a unilateral cross-tibia fracture was made at the proximal right tibia. Animals were then sacrificed after 5 weeks of oral treatment. X-rays were taken at 1 week, 3 weeks and 5 weeks of treatment, and dual energy X-ray absorptiometry was used to measure the bone mineral density (BMD). Changes to the osteocalcin (BGLAP), alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) and estradiol levels in blood were measured. Callus formation and bone union were observed, the BMD was significantly higher and the BGLAP, ALP and TRAP levels were reduced, but no significant increase was observed in the blood estradiol level in the OVX + ICA group compared with the OVX group. The present findings indicate that icariin has potential as a novel alternative therapeutic agent for fracture healing in postmenopausal osteoporosis.

show abstract

“…The tibia was divided into five areas, and the region of interest (ROI) was set as the proximal two areas using a modified division method [19]. The same technician, who was blinded to the treatment assignments, conducted the DXA analyses.…”

Section: Methodsmentioning

confidence: 99%

A Convenient In Vivo Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function

et al. 2016

View full text Add to dashboard Cite

It is difficult to study bone in vitro because it contains various cell types that engage in cross-talk. Bone biologically links various organs, and it has thus become increasingly evident that skeletal physiology must be studied in an integrative manner in an intact animal. We developed a model using local intraosseous small interfering RNA (siRNA) injection to rapidly assess the effects of a target gene on the local skeletal environment. In this model, 160-g male Sprague-Dawley rats were treated for 1–2 weeks. The left tibia received intraosseous injection of a parathyroid hormone 1 receptor (Pth1r) or insulin-like growth factor 1 receptor (Igf-1r) siRNA transfection complex loaded in poloxamer 407 hydrogel, and the right tibia received the same volume of control siRNA. All the tibias received an intraosseous injection of recombinant human parathyroid hormone (1–34) (rhPTH (1–34)) or insulin-like growth factor-1 (IGF-1). Calcein green and alizarin red were injected 6 and 2 days before euthanasia, respectively. IGF-1R and PTH1R expression levels were detected via RT-PCR assays and immunohistochemistry. Bone mineral density (BMD), microstructure, mineral apposition rates (MARs), and strength were determined by dual-energy X-ray absorptiometry, micro-CT, histology and biomechanical tests. The RT-PCR and immunohistochemistry results revealed that IGF-1R and PTH1R expression levels were dramatically diminished in the siRNA-treated left tibias compared to the right tibias (both p<0.05). Using poloxamer 407 hydrogel as a controlled-release system prolonged the silencing effect of a single dose of siRNA; the mRNA expression levels of IGF-1R were lower at two weeks than at one week (p<0.01). The BMD, bone microstructure parameters, MAR and bone strength were significantly decreased in the left tibias compared to the right tibias (all p<0.05). This simple and convenient local intraosseous siRNA injection model achieved gene silencing with very small quantities of siRNA over a short treatment period (≤7 days).

show abstract

The Relationship between Salivary Calcium Concentration and Differences in Bone Mineral Density Level in Female Rats

Cited by 7 publications

References 27 publications

The effects of royal jelly protein on bone mineral density and strength in ovariectomized female rats

The effects of royal jelly protein on bone mineral density and strength in ovariectomized female rats

Effect of icariin on fracture healing in an ovariectomized rat model of osteoporosis

A Convenient In Vivo Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function

Contact Info

Product

Resources

About