Objective: This study involved a situation analysis of nursing students’ preparation and demand for interprofessional practice (IPP) with an aim to make improvements to the interprofessional education (IPE) curriculum. Methods: This was a situation analysis involving 58 responses (75.32%) from the Faculty of Nursing, Mahidol University, Thailand, performed between August and December 2019. Personal information, closed-ended questionnaires, and descriptive questionnaires were utilized to assess the participants’ perspectives regarding their approach to their preparation and demand for IPP. Reflection and interpretation methods were used to categorize the participants’ descriptive answers. Additionally, data are reported as the mean, frequency, percentage, and p-value as appropriate. Results: Overall, 45 (77.6%) participants had never taken the IPE course before. Of those who had taken the IPE course, 22.4% stated that having good communication skills was the main ability needed for multidisciplinary nursing practice in an open-ended question, participants indicated that their self-identity had the key effect on their confidence in multidisciplinary nursing practice (Non-IPE attendants, n = 19 (32.76%), IPE attendants, n = 6 (10.34%)). Furthermore, 77.78% of respondents said they prefer to perform nursing practice to prepare themselves to work with other healthcare professionals collaboratively. Conclusion: IPE not only benefits healthcare students by preparing them to be able to work in their field but also prepares them to be able to collaboratively operate with different healthcare personnel. In particular, communication skills, self-confidence, and nursing practice skills are important attributes that need to be prepared.