The physical activities such as pedaling can affect the lower limb muscles strength and rehabilitation. Improper pedaling can cause injury. In this study, we would investigate the effects of saddle place (saddle position and saddle height) on the behavior of muscles and joints. Moreover, we would try to reveal the relationship between the muscles activity (Act) and the joints reaction forces (F) and saddle position and saddle height. To this end, the pedaling conditions are obtained from the biomechanical model of the human movement system presented in AnyBody software. The variations in 12 muscles Act and total, normal and shear F of ankle, knee and hip joints are studied for the various saddle places in the pedaling feasible range. The relationships of those muscles Act and joints F are predicted by the response surface method. The results indicate that the muscles and the joints behavior changes for various saddle position and saddle height. The maximum and the minimum of the total response are acquired in the ankle and hip joints, respectively. In contrast to the ankle and hip joints, the knee shear response is greater than the normal response. The predictive models of the muscles Act and the joints F (the regression coefficients (R 2) are 0.60-0.95 and 0.76-0.97, respectively) indicate their nonlinear behavior with saddle position and saddle height variations. Studying the muscles and joints behavior in different pedaling condition can be helpful for the suitable saddle placement in order for rehabilitation, muscles soreness reduction, and joints disorder treatment.