The relationship between the mechanical properties and cell behaviour on PLGA and PCL scaffolds for bladder tissue engineering

Baker, Simon C.; Rohman, Géraldine; Southgate, Jennifer; Cameron, Neil R.

doi:10.1016/j.biomaterials.2008.11.033

Cited by 204 publications

(128 citation statements)

References 52 publications

Supporting

Mentioning

114

Contrasting

Unclassified

Order By: Relevance

“…The influence of composition and rigidity of matrices has been shown to be important for support of a number of biological processes [20][21][22][23][24][25][26] and to significantly influence cell differentiation and tissue development. [27][28][29][30][31][32][33][34][35][36][37] We then cultured mESC on AC rat lung matrix (Fig. 4B) and compared cell attachment and survival of cells to mESC cultured on Matrigel (Fig.…”

Section: Comparison Of Mesc Growth On Selected Matricesmentioning

confidence: 99%

Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation

Cortiella

Niles

Cantu

et al. 2010

Tissue Engineering Part A

346

285

View full text Add to dashboard Cite

We report here the first attempt to produce and use whole acellular (AC) lung as a matrix to support development of engineered lung tissue from murine embryonic stem cells (mESCs). We compared the influence of AC lung, Gelfoam, Matrigel, and a collagen I hydrogel matrix on the mESC attachment, differentiation, and subsequent formation of complex tissue. We found that AC lung allowed for better retention of cells with more differentiation of mESCs into epithelial and endothelial lineages. In constructs produced on whole AC lung, we saw indications of organization of differentiating ESC into three-dimensional structures reminiscent of complex tissues. We also saw expression of thyroid transcription factor-1, an immature lung epithelial cell marker; prosurfactant protein C, a type II pneumocyte marker; PECAM-1/CD31, an endothelial cell marker; cytokeratin 18; a-actin, a smooth muscle marker; CD140a or platelet-derived growth factor receptor-a; and Clara cell protein 10. There was also evidence of site-specific differentiation in the trachea with the formation of sheets of cytokeratinpositive cells and Clara cell protein 10-expressing Clara cells. Our findings support the utility of AC lung as a matrix for engineering lung tissue and highlight the critical role played by matrix or scaffold-associated cues in guiding ESC differentiation toward lung-specific lineages.

show abstract

Section: Comparison Of Mesc Growth On Selected Matricesmentioning

confidence: 99%

Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation

Cortiella

Niles

Cantu

et al. 2010

Tissue Engineering Part A

346

285

View full text Add to dashboard Cite

show abstract

“…Furthermore, the material should be biodegradable to support the replacement by normal tissue. Several attempts have been made to identify such an ideal scaffold, such as decellularized matrices (bladder submucosa or small intestine submucosa) [3][4][5][6], collagen-based [7][8][9], and synthetic materials [10,11]. Natural materials are of significant interest due to the biocompatibility of the substrates themselves and their degradation products.…”

Section: Introductionmentioning

confidence: 99%

Microstructure and cytocompatibility of collagen matrices for urological tissue engineering

et al. 2010

View full text Add to dashboard Cite

RESULTS• The best cytocompatibility for both urinary tract-specific cell types was obtained with OptiMaix® (Matricel GmbH, Herzogenrath, Germany) materials.• Cell-specific phenotypes were maintained during culture as shown by immunohistochemical staining.• Furthermore, simultaneous cultivation of both cell types for 7 and 14 days significantly reduced urea permeability. CONCLUSION• These results show the potential of OptiMaix materials in tissue engineering approaches of urinary tract tissues.

show abstract

“…However, cell and tissue incompatibilities appear to be the major restrictions of these materials, leading to the development of other synthetic polymers, such as PLGA (62)(63)(64)(65). These materials were designed specifically to possess adequate structural and biological properties, which can be manipulated for optimal cell proliferation and differentiation (66). In addition, these scaffolds are safe, possess controlled properties of degradation rate and strength, are readily available and possess the ability to carry vital growth factors, such as vascular endothelial growth factor, for neovascularisation (67)(68)(69).…”

Section: Naturally Derived Matricesmentioning

confidence: 99%

Bladder reconstruction: The past, present and future

2015

View full text Add to dashboard Cite

Abstract. Ileal conduit urinary diversion is the gold standard treatment for urinary tract reconstruction following cystectomy. This procedure uses gastrointestinal segments for bladder augmentation, a technique that is often associated with significant complications. The substantial progression in the fields of tissue engineering and regenerative medicine over the previous two decades has resulted in the development of techniques that may lead to the construction of functional de novo urinary bladder substitutes. The present review identifies and discusses the complications associated with current treatment options post-cystectomy. The current techniques, achievements and perspectives of the use of biomaterials and stem cells in the field of urinary bladder reconstruction are also reviewed.

show abstract

The relationship between the mechanical properties and cell behaviour on PLGA and PCL scaffolds for bladder tissue engineering

Cited by 204 publications

References 52 publications

Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation

Influence of Acellular Natural Lung Matrix on Murine Embryonic Stem Cell Differentiation and Tissue Formation

Microstructure and cytocompatibility of collagen matrices for urological tissue engineering

Bladder reconstruction: The past, present and future

Contact Info

Product

Resources

About