Background: In recent years, many literatures have pointed out that the fruit of Prunus cerasifera Ehrhar f. has anti-oxidation effect. Based on the anti-oxidation principle of traditional Chinese medicine in antiviral aspect the antiviral effect of the fruit was studied herein and the problem of the toxicity of the fruit has not been systematically investigated yet. To clarify the toxic effects of the fruit and its material basis, the toxicity test of the fruit was carried out.
Methods:The fruit was percolated with water and the percolated liquid was concentrated to collect the concentrated liquid. Viral infection model in vitro was used to evaluate the antiviral effects of different viruses in vitro. CPE was observed under a microscope to determine the inhibitory effects of different viruses. The best type of macro-porous resin was determined by the method of macro-porous resin adsorption. The types of active ingredients were determined by physical and chemical identification, and their contents were determined by UV. By intragastric administration the long-term toxicity of percolating juice of the fruit to rats was observed including the nature severity, dose-toxicity relationship, time-toxicity relationship, main target organs, toxicity reversible degree, delayed toxicity, etc.Results: showed that percolation solution of the fruit had obvious inhibitory effect on HSV-1 virus, and the TI value was 109.22. The inhibition effect of 60% ethanol precipitation on HSV-1 was the best, and the TI value was 148.84. D101 macro-porous resin presented an excellent separation effect at the volume of the first column eluted by 25% ethanol and TI value was 200.85. Chemical polyphenols were determined by physical and chemical identification and the content was 18.15 mg/g. Comparison of each dose group of percolation of the fruit and blank group there were not abnormalities in appearance signs, behavioral activities, body weight, food intake, hematological examination, serum biochemistry, organ quality and organ coefficient; Morphological examination of organs such as heart, liver, spleen, lung, kidney, ovary, testis, etc. didn't show pathological changes related to medication; Reversible observation showed that the fruit did not cause delayed toxicity to rats. There was not statistically significant difference in the above indicators.
Conclusion:Polyphenols from 25% alcohol elution site of percolation of the fruit may have good anti-HSV-1 activity. The large dose of percolation of the fruit and long-term use don't have obvious toxicity which provides a basis for clinical application.
