The relationship of Cu2+ content to the radioresistance of yeast

Gesswagner, D.; Altmann, Hans; Szilvinyi, A. v.; Kaindl, K.

doi:10.1016/0020-708x(68)90083-5

Cited by 7 publications

(1 citation statement)

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“…ellipsoideus (ATCC 560) and E. coli K-12 his-(ATCC 25290) were obtained from the American Type Culture Collection, Rockville, Md. Yeast extract and Trypticase soy broth were products of Becton, Dickinson and Co., and agar was purchased from Difco, Inc. Puromycin hydrochloride was obtained from Nutritional Biochemicals, Inc. Yeast were grown in the semisynthetic medium of Gesswagner et al (6). Inocula of 0.2 -0.5% of the total broth volume were used, and growth was accomplished at 34 C on a gyratory shaker for 24 to 48 h. When the yeast were to be grown anaerobically, the Gesswagner medium was supplemented with 1 g of Tween 80 and 30 mg of ergosterol per liter in order to compensate for the inability of these cells to synthesize sterols in the absence of oxygen (15).…”

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Superoxide Dismutase and Oxygen Toxicity in a Eukaryote

1974

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Saccharomyces cerevisiae var. ellipsoideus contained 6.5 times more superoxide dismutase and 2.3 times more catalase when grown under 100% 02 than when grown anaerobically. Growth under oxygen caused equal increases in both the cyanide-sensitive and the cyanide-insensitive superoxide dismutases of this organism. Experience with other eukaryotes has shown that cyanide sensitivity is a property of the cupro-zinc superoxide dismutase of the cytosol, whereas cyanide insensitivity is a property of the corresponding mangani-enzyme found in mitochondria. Cu2+, which has been shown to increase the radioresistance of yeast, also caused an increase of both of the superoxide dismutases of S. cerevisiae. Yeast which had been grown under 1 atm of 02 were more resistant toward the lethal effects of 20 atm of 02 than were yeast which had been grown in the absence of 0,. Escherichia coli K-12 his-responded to growth under 1 atm of 02 by increasing its content of catalase and of peroxidase, but not of superoxide dismutase. This contrasts with E. coli B, which was previously shown to respond to 02 by a striking increase in superoxide dismutase. E. coli K-12 his-did not gain resistance toward 20 atm of 02 because of having been grown under 1 atm of 02. Once again, this contrasts with the behavior of E. coli B. These data indicate that, in both prokaryotes and in eukaryotes, superoxide dismutase is an important component of the defenses against oxygen toxicity.

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confidence: 99%