The Relationship of Growth and Adenylate Cyclase Activity in Cultured Thyroid Cells: Separate Bioeffects of Thyrotropin

Valente, William A.; Vitti, Paolo; Kohn, Leonard D.; Brandi, Maria Luisa; Rotella, Carlo Maria; Toccafondi, R; Tramontano, Donatella; Aloj, Salvatore M.; Ambesi-Impiombato, Francesco Saverio

doi:10.1210/endo-112-1-71

Cited by 215 publications

(77 citation statements)

References 32 publications

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“…Many studies indicate that TSH has TGA (Drexhage et al, 1980;Valente et al, 1983b;Roger and Dumont, 1984;Stringer et al, 1985;Dere and Rapoport, 1986 ;Jin et al, 1986;Ealey et al, 1987Tramontano et al, 1988Zakarija and Mckenzie, 1989), including [3H] Tdr incorporation in FRTL-5 cells as a convenient and reproducible index (Valente et al, 1983a;Jin et al, 1986;Tramontano et al, 1988;Zakarija and Mckenzie, 1989). In the present study, we showed that not only TSH but also hCG has TGA by measuring [3H] Tdr incorporation in FRTL-5 cells, which is consistent with other reports (Davies and Platzer, 1986;Hershman et al, 1988).…”

Section: Discussionsupporting

confidence: 92%

Human Chorionic Gonadotropin Promotes Thyroid Growth via Thyrotropin Receptors in FRTL-5 Cells.

et al. 1990

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Section: Discussionsupporting

confidence: 92%

Human Chorionic Gonadotropin Promotes Thyroid Growth via Thyrotropin Receptors in FRTL-5 Cells.

et al. 1990

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“…A rat thyroid cell line (FRTL) requires TSH and insulin in order to proliferate in a lowserum-hormone-supplemented medium [ 151, similarly to observations presented here. IN FRTLS, a cloned cell line derived from FRTL and adapted to grow in presence of 5% serum, dbcAMP does not reproduce the mitogenic effect of TSH [33] leading to the conclusion that TSH stimulates thyroid cell proliferation acting at least in part by CAMP-independent mechanisms. Obviously, such negative evidence needs to be confirmed with more potent and specific stimulations of the cyclic AMP system.…”

Section: Discussionmentioning

confidence: 99%

Stimulation by thyrotropin and cyclic AMP of the proliferation of quiescent canine thyroid cells cultured in a defined medium containing insulin

Roger¹,

Servais²,

Dumont³

1983

FEBS Letters

145

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We have developed serum-free primary cultures of differentiated follicular dog thyroid cells which allow the study of the hormonal control of cell proliferation. The cooperation of insulin and increasing cellular cyclic AMP by thyrotropin triggers the DNA synthesis and the proliferation. Dog thyroid cells are an example of a system in which cyclic AMP is a sufficient signal to stimulate the proliferation in quiescent cells. Thyroid

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“…In vitro studies, however, failed to demonstrate the same results in cultured human thyrocytes (Dumont, 1971;Valente et al, 1983;Wadeleux and Winard, 1981;Westermark et al, 1979). The addition of cyclic adenosine monophosphate (cAMP) also suppressed Fas-mediated apoptosis of thyrocytes, and both TSH and TSAb containing IgG increased cAMP level in thyrocytes Kawakami et al, 1997b), suggesting that TSH and TSAb act on thyrocytes in a similar manner to functional Fas expression on thyrocytes.…”

Section: Kawakami Et Almentioning

confidence: 99%

CD4+ T Cell-Mediated Cytotoxicity Toward Thyrocytes: The Importance of Fas/Fas Ligand Interaction Inducing Apoptosis of Thyrocytes and the Inhibitory Effect of Thyroid-Stimulating Hormone

Kawakami

Matsuoka

Tsuboi

et al. 2000

Laboratory Investigation

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SUMMARY:The accumulation of activated CD4 ϩ T cells and antigen (Ag)-dependent cellular interactions between thyrocytes and CD4 ϩ T cells have been determined in thyroid gland from patients with Graves' disease. The Fas/Fas ligand (FasL) interaction between antigen-presenting cells and T cells regulates the apoptosis of the former cells triggered by the latter cells. The inhibition of Fas-mediated apoptosis in thyrocytes could be a underlying mechanism of hyperplasia of thyrocytes in patients with Graves' disease. We investigated the potential role of Fas/FasL interaction between thyrocytes and CD4 ϩ T cells in the induction of Fas-mediated apoptosis of the former cells induced by the latter cells. The presence of only a few specific T cells responsive to a putative autoantigen has hampered the investigation of specific T cell activation toward antigen-presenting cells (APCs). Therefore, we used a superantigen, staphylococcal enterotoxin B (SEB), to examine specific T cell activation toward thyrocytes in vitro since it stimulates a large proportion of T cells with particular V␤ elements. Spontaneous apoptosis of thyrocytes in culture was not found even in the presence of various kinds of cytokines. In contrast, a clear induction of Fas-mediated apoptosis by anti-Fas IgM was determined in interferon-␥ (IFN-␥)-stimulated thyrocytes. In addition, a significant cytotoxicity of purified CD4 ϩ T cells toward IFN-␥-stimulated thyrocytes in the presence of SEB was induced, and the addition of anti-HLA-DR and -DQ monoclonal antibodies (mAbs) or blockade of the Fas/FasL interaction reduced this cytotoxicity. FasL expression of CD4 ϩ T cells cocultured with IFN-␥-stimulated thyrocytes in the presence of SEB was clearly induced. Furthermore, the addition of mAbs against CD54 and CD58 inhibited both cytotoxicity and FasL expression of CD4 ϩ T cells. The cytotoxicity of CD4 ϩ T cells toward IFN-␥-stimulated, SEB-pulsed thyrocytes was markedly inhibited when we used thyrocytes cultured with IFN-␥ in the presence of thyroid-stimulating hormone (TSH) as target cells. Our results suggest that 1) CD4 ϩ T cells were activated by thyrocytes expressing MHC class II molecules in an SEB-dependent manner and then expressed FasL. 2) These activated FasL ϩ CD4 ϩ T cells killed thyrocytes by interacting with Fas on thyrocytes and FasL on activated CD4 ϩ T cells. The presence of costimulating molecules such as CD54 and CD58 on thyrocytes was also necessary to generate activated FasL ϩ CD4 ϩ T cells. 3) Since the actions of thyroid stimulating antibody (TSAb) toward thyrocytes are similar to those of TSH, one goitrogenic activity of TSAb may, in part, be due to the inhibitory effect on Fas-mediated apoptosis of thyrocytes triggered by activated CD4 ϩ T cells. (Lab Invest 2000, 80:471-484).

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The Relationship of Growth and Adenylate Cyclase Activity in Cultured Thyroid Cells: Separate Bioeffects of Thyrotropin

Cited by 215 publications

References 32 publications

Human Chorionic Gonadotropin Promotes Thyroid Growth via Thyrotropin Receptors in FRTL-5 Cells.

Human Chorionic Gonadotropin Promotes Thyroid Growth via Thyrotropin Receptors in FRTL-5 Cells.

Stimulation by thyrotropin and cyclic AMP of the proliferation of quiescent canine thyroid cells cultured in a defined medium containing insulin

CD4+ T Cell-Mediated Cytotoxicity Toward Thyrocytes: The Importance of Fas/Fas Ligand Interaction Inducing Apoptosis of Thyrocytes and the Inhibitory Effect of Thyroid-Stimulating Hormone

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