The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores

Abstract

Objective:We aimed to evaluate the relationship of micronucleus (MN) frequency and nuclear division index (NDI) with SYNTAX and Gensini scores and thrombolysis in myocardial infarction (TIMI) frame counts of coronary arteries in patients undergoing coronary angiography.Methods:In a single-center prospective observational study, a total of 63 individuals, 48 consecutive patients with coronary artery disease (CAD) and 15 healthy people were included. Before coronary angiography (exposure to X-ray), blood samples were collected for lymphocyte cultures, MN and NDI measurements. According to the SYNTAX and Gensini scores, patients were allocated into two groups. Group 1 and 2 included the patients with SYNTAX scores <22 and ≥22 points, respectively. Similarly, groups according to Gensini scores included the ones <23 and ≥23 points. MN test was used for in vitro studies in human peripheral lymphocytes. Binucleated lymphocytes were calculated for each patient.Results:MN frequency was significantly higher in group 2 than group 1 and in group 1 than control group (p<0.001). NDI was significantly higher in control group than group 1 and in group 1 than group 2 (p=0.003). MN frequency had positive but moderate correlation with SYNTAX and Gensini scores and TFCs of left anterior descending (LAD), circumflex and right coronary arteries (r=0.394, p=0.003; r=0.458, p<0.001; r=0.425, p<0.001; r=0.469, p<0.001; and r=0.475, p<0.001, respectively).Conclusion:We can conclude that as the degree of atherosclerosis increases and coronary flow worsens, MN frequency increases and NDI decreases. Our results may help to elucidate the relationship of DNA damage in pathophysiology of atherosclerosis and endothelial dysfunction in patients with stable CAD.

CGAS is a micronucleophagy receptor for the clearance of micronuclei

“…Micronuclei usually originate from mis-segregation of DNA during cell division and appear as small membrane-enclosed cytoplasmic bodies containing whole or fragmented chromosomes that are surrounded by its own nuclear membrane [ 14 ]. Increased frequency of micronuclei is associated with many diseases including cancer [ 15 ], inflammation and autoimmune diseases [ 16 , 17 ], neurodegenerative diseases [ 18 ], metabolic diseases [ 19 , 20 ] and cardiovascular diseases [ 21 ]. Though recent work indicate that micronuclei can be subjected to autophagic degradation [ 22 , 23 ], the autophagy receptor responsible for the clearance of micronuclei remains unknown.…”

Comparison of Machine Learning Models and Framingham Risk Score for the prediction of the presence and severity of Coronary Artery Diseases by using Gensini Score

“…For instance, in a large Chinese cohort study (15), the data show the correlation between the ABO blood groups and the severity of CAD; And about the obesity with CAD, the Waist-Hip Ratio is considered to positively related to the presence and severity of CAD(16). HDL sub-fractions (17) and micronucleus frequency and nuclear division index (18)are also proved to be the significant indicators to predict the extent and severity of CAD. In the future further study, to add up these special variables to the dataset was a promising step for the risk prediction.…”

Evaluation of cytogenetic alterations of toxic gas exposed population of Bhopal having chronic kidney disease

“…An exposure of metals in CKD patients can leads to reductions in kidney functions (Orr and Bridges, 2017).Thus, the confounders can be a lifestyle, living environment, nutritional factors, drinking water and occupational exposure to other toxicants . Ipek et al, (2017) opined that alterations in NDI value aredirectly related to the proliferative ability of the cell. The urinary cell free mitochondrial DNA and nuclear DNA could be employed as prognostic biomarkers for kidney outcome in CKD (Chang et al, 2019).…”