The Relationship of Plasma miR-29a and Oxidized Low Density Lipoprotein with Atherosclerosis

Abstract

Background/Aims: Atherosclerosis is a chronic inflammatory condition associated with a variety of vascular diseases. Previous studies showed that both miR-29a and oxidized low density lipoprotein (ox-LDL) were vital in the development of atherosclerosis. However, the relationship between miR-29a and ox-LDL remains unknown. This study was designed to investigate the association of miR-29a and ox-LDL and to test whether circulating miR-29a and ox-LDL levels could predict atherosclerosis. Methods: In 170 participants, plasma levels of miR-29a were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) while plasma ox-LDL levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. The relationship between miR-29a level and ox-LDL and carotid intima-media thickness (cIMT) was assessed using the Spearman correlation coefficient and multiple liner regression. Results: Compared with the normal cIMT group, the increased cIMT group had higher levels of ox-LDL (0.47 ± 0.08 vs 0.29 ± 0.06 ng/ml, p = 0.003) and miR-29a (32.93 ± 4.26 vs 26.37 ± 1.04, p < 0.001). A positive correlation was found between ox-LDL and miR-29a (r = 0.695, p < 0.001), and both the ox-LDL (r = 0.857, p < 0.001) and the miR-29a (r = 0.753, p < 0.001) were positively related to cIMT. Furthermore, multiple liner regression indicated that a significant correlation between ox-LDL and cIMT (β = 0.768, p < 0.001), as well as between miR-29a and cIMT (β = 0.686, p <0.001). The combination of miR-29a and ox-LDL (AUC = 0.926, p < 0.001) offered a better predictive value for atherosclerosis than either miR-29a (AUC = 0.759, p < 0.001) or ox-LDL (AUC = 0.762, p < 0.001) alone. Conclusion: Increased miR-29a and ox-LDL levels were associated with an early stage of atherosclerosis, and the combination of miR-29a and ox-LDL offered better predictive values for atherosclerosis than either alone.

Low-Intensity Pulsed Ultrasound Prevents the Oxidative Stress Induced Endothelial-Mesenchymal Transition in Human Aortic Endothelial Cells

“…Oxidative stress, as an adaptive response or repair mechanism, has been reported to play a role in the pathogenesis of various cardiovascular disease including coronary artery disease, hypertension and diabetic cardiomyopathy [28, 29]. Oxidative stress-induced injuries of the vascular endothelium may be an initial step in the pathogenesis of cardiovascular disease [30].…”

Akt3 kinase suppresses pinocytosis of low-density lipoprotein by macrophages via a novel WNK/SGK1/Cdc42 protein pathway

“…Interestingly, miR-29 has been found to exert pro-atherosclerotic effects. Enhanced miR-29a levels were associated with an early stage of atherosclerosis (39). Sustained administration of locked nucleic acid (LNA)-miR-29, an miR-29 inhibitor, into an atherosclerotic mouse model improves indices of plaque morphology (40).…”

PTPRO Promotes Oxidized Low-Density Lipoprotein Induced Oxidative Stress and Cell Apoptosis through Toll-Like Receptor 4/Nuclear Factor κB Pathway

“…It has been generally established that AS is an inflammatory disease involving a chronic persistent state. AS commonly initiates within the arterial wall primarily as a result of the destruction occurring in endogenously structures, which can be attributed to several matters, such as hypertension, smoking, oxidized low-density lipoprotein (ox-LDL) and so forth [3-5]. Ox-LDL, as the most important factor contributing to the development of AS, can activate both the innate and adaptive immune responses with the differentiation from monocytes to macrophages and the unregulated phagocytic activity of macrophages [6-8], subsequently induce the accumulation of lipid and the formation of foam cells, which is the hallmark event of AS [9, 10].…”