The relationship of pork longissimus muscle pH to mitochondrial respiratory activities, meat quality and muscle structure

Popp, Johanna; Wicke, Michael; Klein, Günter; Krischek, Carsten

doi:10.1017/s1751731114002365

Cited by 18 publications

(13 citation statements)

References 42 publications

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“…The enzymes in the cytoplasm generally play an essential role in a neutral environment. Lower pH caused the active core group of the enzyme to deviate from its optimal dissociation state, reducing its capacity to bind to the substrate and catalytic activity 25,35 . In the present study, the fast pH decline group had greater ROS levels than the slow group, which could be owing to changes in the structure and activity of ROS scavenging enzymes by rapidly declining muscle pH.…”

Section: Resultsmentioning

confidence: 99%

The effect of postmortem pH decline rate on mitochondrial apoptosis and tenderness of bovine skeletal muscle during aging

Han³

et al. 2021

Preprint

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This study aimed to investigate the effect of postmortem pH decline rate on mitochondrial dysfunction-induced apoptosis and bovine muscle tenderness during aging. Protein denaturation, reactive oxygen species (ROS) levels, mitochondrial apoptosis factors, and shear force were assessed in bovine muscles with different pH decline rates. The results showed that compared to the slow group, fast pH decline in bovine muscle significantly increased sarcoplasmic protein denaturation at 6–12 h and mitochondrial ROS levels at 6–24 h (P<0.05), and in turn significantly accentuated mitochondrial dysfunction and cytochrome c oxidation levels (P<0.05), resulting in caspase-3 activation, apoptosis, and reduced muscle shear force (P<0.05). These results demonstrated that the fast postmortem pH decline enhanced mitochondrial apoptosis and bovine muscle tenderization by inducing ROS accumulation during aging.

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Section: Resultsmentioning

confidence: 99%

The effect of postmortem pH decline rate on mitochondrial apoptosis and tenderness of bovine skeletal muscle during aging

Han³

et al. 2021

Preprint

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“…Phung et al (2013) showed that bovine semimembranosus temperature was negatively associated with OCR. Others have observed that porcine LL with rapid pH decline exhibits a more profound decrease in mt respiratory activity in comparison to LL with slower pH decline (Werner et al, 2010;Popp et al, 2015). Paradoxically, rapid pH decline in Dia does not appear to negatively impact mt function.…”

Section: Muscle Ph and Temperature Declinementioning

confidence: 94%

Mitochondrial Function in Oxidative and Glycolytic Bovine Skeletal Muscle Postmortem

Ramos

Bell

Wohlgemuth

et al. 2021

Meat and Muscle Biology

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Meat quality is traditionally associated with anaerobic metabolism due to cessation of the oxygen supply post-mortem. However, mitochondrial (mt) function early postmortem may affect the development of meat quality characteristics, such as adenosine triphosphate levels and pH decline. Therefore, the objective of this study was to evaluate mt function ex vivo during the first 24 h postmortem in muscles with differences in mt content. Samples from longissimus lumborum (LL) and diaphragm (Dia) were taken from steers (n = 6) at 1, 3, and 24 h postmortem and frozen to determine citrate synthase (CS) activity and mt protein expression (immunodetection) or were fresh-preserved for high-resolution respirometry. Integrative oxygen consumption rate (picomoles per second per milligram of tissue) was measured and normalized to CS activity as a proxy for mt content (intrinsic mt function, picomoles per second per unit CS). CS activity (P < 0.001) and mt protein expression (P < 0.001) were greater in Dia, which was reflected in mt respiration. Muscle type affected (P < 0.001) integrative leak respiration and was greater in mt from Dia; oxidative phosphorylation (OXPHOS) was also greater in Dia and influenced by time postmortem (muscle × time: P = 0.01). Intrinsic leak and OXPHOS were affected by muscle and time (muscle × time: P = 0.05 and P = 0.01, respectively), with the most pronounced differences at 24 h postmortem. Stimulation of OXPHOS by cytochrome c as an indicator of outer mt membrane integrity was influenced by muscle and time postmortem (muscle × time: P = 0.03); it was greater in mt from LL. Despite intrinsic differences in respiratory function at 24 h, mt from both muscles were intact and coupled at 1 h postmortem. Reduced content and respiratory function in mt from LL are associated with early fragmentation, which could impact protease activation and subsequently meat quality.

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“…Mitochondria are also known to play a role in postmortem pH decline (Popp et al, 2015;Matarneh et al, 2016). Mitochondria influence pH decline because functioning mitochondria will allow pyruvate to be metabolized by the citric acid cycle instead of being converted to lactate.…”

Section: Supplemental Tablementioning

confidence: 99%

Metabolomic and proteomic features of fresh pork loin that vary in ultimate pH and pork quality

Zuber¹

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The relationship of pork longissimus muscle pH to mitochondrial respiratory activities, meat quality and muscle structure

Cited by 18 publications

References 42 publications

The effect of postmortem pH decline rate on mitochondrial apoptosis and tenderness of bovine skeletal muscle during aging

The effect of postmortem pH decline rate on mitochondrial apoptosis and tenderness of bovine skeletal muscle during aging

Mitochondrial Function in Oxidative and Glycolytic Bovine Skeletal Muscle Postmortem

Metabolomic and proteomic features of fresh pork loin that vary in ultimate pH and pork quality

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