The Relationship of Prevotella intermedia, Prevotella nigrescensand Prevotella melaninogenica in the Supragingival Plaque of Children, Caries and Oral Malodor

Abstract

Purpose: A relationship between the distribution of periodontal bacteria species and malodor in children has not been sufficiently investigated. The present study was undertaken to determine the presence of 3 periodontopathic bacteria (Prevotella spp. P. intermedia, P. nigrescens, P. melaninogenica) in the supragingival plaques of 3 to 16-year-old children with different oral health conditions and oral malodor. Methods: The number of decayed and filled primary teeth (df) and Decayed, Missing and Filled permanent teeth (DMF),Papillary Marginal and Attached gingivitis (PMA) index, Oral Hygiene Index (OHI), and oral malodor of each subject were determined prior to the collection of supragingival plaques. Three periodontopathic bacteria(P. intermedia, P. nigrescens, P. melaninogenica ) in supragingival plaques were detected by using an immunoslot blot assay with monoclonal antibodies specific for each microorganism. Findings: The frequencies of periodontopathic bacteria in children with and without caries were not significantly different from each other. Positivity for P. intermedia, but not for P. nigrescens or P. melaninogenica was correlated with oral malodor. Oral malodor was also correlated with the debris index, a component of OHI. The group with the higher OHI showed a higher prevalence of periodontopathic bacteria. For the 3 periodontopathic bacteria in the subjects tested, plaques positive for any of them were not age related. However,the frequencies of all 3 periodontopathic bacteria were the highest in the 3-6-year olds. Conclusion: The supragingival plaques in children can harbor 3 species of periodontopathic bacteria, P. intermedia,P. nigrescens, and P. melaninogenica.

Accurate genome relative abundance estimation for closely related species in a metagenomic sample

“…This is consistent with the previous findings [24, 25], and it is well known that Prevotella denticola is a bacterial species found in the human mouth that causes infections of the oral cavity and adjacent structures [26]. We also noticed that the species Leptotrichia buccalis in the class of Fusobacteria is more abundant in the cavity samples than in the healthy controls, which is not surprised since it is the first species in the genus Leptotrichia found in human dental plaque [27].…”

Oral Bacterial Communities in Individuals with Type 2 Diabetes Who Live in Southern Thailand

“…Other information that related to a person's background and behavior (such as family history of DM, history of excessive consumption of sweet foods, history of smoking, and dry mouth) was recorded before the dental examination and treatment were completed. Caries prevalence was recorded according to the DMFT index, where D refers to the number of untreated carious teeth, F represents the number of filled teeth, M refers to the number of teeth extracted because of caries, and T refers to the total number of teeth (19)(20)(21)(22)(23). The DMFT index provides an indication of caries experience (current or previous caries activity).…”

High dietary salt–induced DC activation underlies microbial dysbiosis-associated hypertension

“…Bacteria of the families Lachnospiraceae and Prevotellaceae have been linked to metabolic syndrome and chronic inflammation, including periodontal disease and rheumatoid arthritis (20)(21)(22). Mice fed a high-salt diet showed marked increases in Firmicutes bacteria belonging to class Clostridia, order Clostridiales, family Lachnospiraceae and the genera Lachnospiraceae UCG-006, Incertae Sedis, and the Lachnospiraceae FCS020 group ( Figure 6D).…”