The relationship of TRP channels to the pacemaker activity of interstitial cells of Cajal in the gastrointestinal tract

Kim, Byung Joo; So, Insuk; Kim, Ki Whan

doi:10.1540/jsmr.42.1

Cited by 34 publications

(39 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, in interstitial cells of Cajal, TRPC4 was suggested as a molecular candidate for the nonselective cation channel responsible for the pacemaker activity (14). In the entorhinal cortex, TRPC cation channels mediating persistent muscarinic currents contribute significantly to the firing and mnemonic properties of projection neurons (44).…”

Section: Discussionmentioning

confidence: 99%

Role of nonselective cation channels in spontaneous and protein kinase A-stimulated calcium signaling in pituitary cells

Tomić

Kučka

Kretschmannova

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

-Several receptors linked to the adenylyl cyclase signaling pathway stimulate electrical activity and calcium influx in endocrine pituitary cells, and a role for an unidentified sodium-conducting channel in this process has been proposed. Here we show that forskolin dose-dependently increases cAMP production and facilitates calcium influx in about 30% of rat and mouse pituitary cells at its maximal concentration. The stimulatory effect of forskolin on calcium influx was lost in cells with inhibited PKA (cAMP-dependent protein kinase) and in cells that were haploinsufficient for the main PKA regulatory subunit but was preserved in cells that were also haploinsufficient for the main PKA catalytic subunit. Spontaneous and forskolin-stimulated calcium influx was present in cells with inhibited voltage-gated sodium and hyperpolarization-activated cation channels but not in cells bathed in medium, in which sodium was replaced with organic cations. Consistent with the role of sodium-conducting nonselective cation channels in PKA-stimulated Ca 2ϩ influx, cAMP induced a slowly developing current with a reversal potential of about 0 mV. Two TRP (transient receptor potential) channel blockers, SKF96365 and 2-APB, as well as flufenamic acid, an inhibitor of nonselective cation channels, also inhibited spontaneous and forskolin-stimulated electrical activity and calcium influx. Quantitative RT-PCR analysis indicated the expression of mRNA transcripts for TRPC1 ϾϾ TRPC6 Ͼ TRPC4 Ͼ TRPC5 Ͼ TRPC3 in rat pituitary cells. These experiments suggest that in pituitary cells constitutively active cation channels are stimulated further by PKA and contribute to calcium signaling indirectly by controlling the pacemaking depolarization in a sodiumdependent manner and directly by conducting calcium.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of nonselective cation channels in spontaneous and protein kinase A-stimulated calcium signaling in pituitary cells

Tomić

Kučka

Kretschmannova

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Expression of TRPC4 (ENSG00000100991; TRPC4) is found in endothelium and smooth muscle cells (Beech 2005;Tiruppathi et al 2006), intestinal pacemaker cells (ICC) (Kim et al 2006), in many brain regions (Zechel et al 2007), adrenal glands (Philipp et al 2000), and in kidneys (Freichel et al 2005). It is suggested that TRPC4 is an essential component of the nonselective cation channel involved in neuromodulation of stomach smooth muscle after muscarinic stimulation (Lee et al 2005).…”

Section: Trpcsmentioning

confidence: 99%

The Role of Transient Receptor Potential Cation Channels in Ca2+ Signaling

Gees

Colsoul²,

Nilius³

2010

Cold Spring Harbor Perspectives in Biology

382

361

View full text Add to dashboard Cite

The 28 mammalian members of the super-family of transient receptor potential (TRP) channels are cation channels, mostly permeable to both monovalent and divalent cations, and can be subdivided into six main subfamilies: the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPP ( polycystin), TRPML (mucolipin), and the TRPA (ankyrin) groups. TRP channels are widely expressed in a large number of different tissues and cell types, and their biological roles appear to be equally diverse. In general, considered as polymodal cell sensors, they play a much more diverse role than anticipated. Functionally, TRP channels, when activated, cause cell depolarization, which may trigger a plethora of voltage-dependent ion channels. Upon stimulation, Ca 2þ permeable TRP channels generate changes in the intracellular Ca 2þ concentration, [Ca 2þ ] i , by Ca 2þ entry via the plasma membrane. However, more and more evidence is arising that TRP channels are also located in intracellular organelles and serve as intracellular Ca 2þ release channels. This review focuses on three major tasks of TRP channels: (1) the function of TRP channels as Ca 2þ entry channels; (2) the electrogenic actions of TRPs; and (3) TRPs as Ca 2þ release channels in intracellular organelles.T ransient receptor potential (TRP) channels constitute a large and functionally versatile family of cation-conducting channel proteins, which have been mainly considered as polymodal unique cell sensors. The first TRP channel gene was discovered in Drosophila melanogaster (Montell and Rubin 1989) in the analysis of a mutant fly whose photoreceptors failed to retain a sustained response to maintained light stimuli. So far, more than 50 TRP channels have been identified with representative members in many species. The evolutionary first TRP channels in protists, chlorophyte algae, choanoflagellates, yeast, and fungi are primary chemo-, thermo-, or mechanosensors (Cai 2008;Wheeler and Brownlee 2008;Chang et al. 2010;Matsuura et al. 2009). Many of these functions are remarkably conserved from protists, worms, and flies to humans (Montell 2005;Pedersen et al. 2005;Nilius et al. 2007;Damann et al. 2008). More than 50 trp genes have been cloned so far that comprise approximately 20% of the known genes encoding ion channels. In mammals, 28 TRP channels were found and classified according to homology into 6 subfamilies: TRPC (canonical), TRPV (vanilloid),

show abstract

“…In the murine small intestine, pacemaker potentials are predominantly induced by the activation of non-selective cation channels (25,26) or Cl -channels (27)(28)(29). To determine which channel is associated with the m-PM-induced depolarization of pacemaker potentials, the effects of m-PM on TRPM7 and Ca 2+ -activated Cl -channels were examined.…”

Section: Association Of Trpm7 and Camentioning

confidence: 99%

“…Additionally, endogenous agents are able to regulate GI motility function via ICCs (22)(23)(24)(25). Furthermore, transient receptor potential melastatin (TRPM) 7 (26) or Cl -channels, such as anoctamin1 (ANO1) (27)(28)(29), are associated with pacemaker potentials in the GI tract. Therefore, TRPM7 and ANO1 may be therapeutic targets for the treatment of GI motility disorders.…”

Section: Introductionmentioning

confidence: 99%

Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine

Lee

Kim

et al. 2016

Experimental and Therapeutic Medicine

Self Cite

View full text Add to dashboard Cite

Abstract. Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode. 4-Diphenylacetoxy-N-methyl-piperidine methiodide, which is a muscarinic 3 (M 3 ) receptor antagonist, was able to block m-PM-induced pacemaker potential increases, whereas methoctramine, which is a muscarinic 2 (M 2 ) receptor antagonist, was not. When 1 mM guanosine diphosphate β-5 was present in the pipette solution, m-PM induced slight pacemaker depolarization. Following pretreatment in bath solution of Ca 2+ -free solution or a Ca 2+ -ATPase inhibitor in endoplasmic reticulum, the pacemaker currents were inhibited. Furthermore, pretreatment with PD98059, SB203580 or SP600125, which is a c-jun NH2-terminal kinase inhibitor, blocked m-PM-induced ICC potential depolarization. Furthermore, m-PM inhibited transient receptor potential melastatin (TRPM) 7 channels, but did not affect Ca 2+ -activated Cl -channels. These results suggest that m-PM is able to modulate pacemaker potentials through the muscarinic M 3 receptor, via G-protein and external and internal Ca 2+ , in a mitogen-activated protein kinase and TRPM7-dependent manner. Therefore, m-PM may provide a basis for the development of a novel gastroprokinetic agent. IntroductionThe fruit of the plum tree Prunus mume Siebold & Zucc. (PM) is used across East Asia, particularly in Korea and Japan (1), as a traditional herbal medicine for the relief of digestive problems, fatigue and fever. PM contains a number of phenolic compounds, including phenolic acids and flavonoids (1,2), which have antioxidant and free radical scavenging activities in vivo (3-6). Furthermore, PM extracts exhibit many pharmacological activities, including antimicrobial (7-10), immune enhancing (11), anti-cancer (1,12,13), and anti-fatigue (14) effects, and have been demonstrated to enhance osteoclast differentiation (15) and improve blood flow (16). Additionally, previous studies have reported that using PM extracts with probiotics inhibits the development of atopic dermatitis (17) and enhances immunity (18).Interstitial cells of Cajal (ICCs) are the pacemakers of the gastro-intestinal tract and generate rhythmic responses in cell membrane electrical potentials (19,20), thus serving important roles in the regulation of GI motility (21). Additionally, endogenous agents are able to regulate GI motility function via ICCs (22-25). Furthermore, transient receptor potential melastatin (TRPM) 7 (26) or Cl -channels, such as anoctamin1 (ANO1) (27)(28)(29), are associated with pacemaker potentials in the...

show abstract

The relationship of TRP channels to the pacemaker activity of interstitial cells of Cajal in the gastrointestinal tract

Cited by 34 publications

References 23 publications

Role of nonselective cation channels in spontaneous and protein kinase A-stimulated calcium signaling in pituitary cells

Role of nonselective cation channels in spontaneous and protein kinase A-stimulated calcium signaling in pituitary cells

The Role of Transient Receptor Potential Cation Channels in Ca2+ Signaling

Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine

Contact Info

Product

Resources

About