The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor

Abstract

Canine mammary tumor (CMT) is the most common tumor in dogs, with 50% of malignant cases, and lacks an effective therapeutic schedule, hence its early diagnosis is of great importance to achieve a good prognosis. Microbiota is believed to play important roles in systemic diseases, including cancers. In this study, 91 tumors, 21 oral and fecal samples in total were collected from dogs with CMTs, and 31 oral and 21 fecal samples from healthy dogs were collected as control. The intratumoral, oral and gut bacterial community of dogs with CMTs and healthy dogs was profiled by 16S rRNA high-throughput sequencing and bioinformatic methods. The predominant intratumoral microbes were Ralstonia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Pseudomonas, unidentified_Chloroplast and Bacteroides at the genus level. In addition, our findings demonstrated striking changes in the composition of the oral and gut bacterium community in the dogs suffered from CMTs compared to the healthy dogs, with a significant increase of Bacteroides which also was the significant microbial biomarker in the oral and gut bacterium community. It showed that the Bacteroides was shared in the intratumoral, oral and intestinal bacterial microbiomes, confirming that microbiota might travel from the mouth to the intestine and finally to the distant mammary tumor tissue. This study provides a new microbiological idea for the treatment of canine mammary tumors, and also provides a theoretical basis for the study of human breast cancer.

Characterization of the Gut Microbiome in Healthy Dogs and Dogs with Diabetes Mellitus

“…This was evident in the distinct clustering of the two groups in the PCoA plots based on Bray-Curtis and Jaccard distances, as well as the differential abundance analysis at the genera level. The phyla Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Proteobacteria were detected in both groups, aligning with prior research on the gut microbiome of canines [16,19,35]. We observed that the family Clostridiaceae was overexpressed, consistent with previous studies on insulin-dependent diabetic dogs [36,37].…”

Black Lycium barbarum polysaccharide attenuates LPS-induced intestine damage via regulation gut microbiota

“…It is assumed that the mere presence or absence of Adlercreutzia rather than its abundance (% age) drives the impact associated with the presence of this particular genus. A slight increase in Bacteroides, Mucispirillum, opportunistic pathogenic Enterococcus, Ruminococcus, and pro-inflammatory bacteria of Desulfovibrio and Helicobacter wwas found in LPSinduced mice, which was in accordance with the results found in dogs with mammary tumor (Zheng et al, 2022), mice with colitis (Lee et al, 2022), patients with atrial fibrillation (Huang et al, 2022), people with obseity (Xu et al, 2022), and in high-fat diet-induced obesity mice. Mice in group GE demonstrated a decrease in those genera to the same level as control in mice in group GA, which may explain that BLBP mediates intestinal damage via a decrease of those relatively negative bacteria in mice.…”

Isolation of Oral Bacteria, Measurement of the C‐Reactive Protein, and Blood Clinical Parameters in Dogs with Oral Tumor

“…In that study, the Bacteroides bacteria were shared in the intratumoral, oral, and gut bacterium community of canine mammary tumor cases. This result confirmed that these bacteria might travel from the gastrointestinal tract to the tumor sites [ 73 ]. This was related to previous study in human oral SCC and the bacterial translocation.…”