The relationships between changes in main biochemical parameters in sperm cells of infertile men

Фафула, Р. В.; Воробец, З. Д.

doi:10.30970/sbi.1301.587

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…The plasma membrane of spermatozoa is enriched in polyunsaturated fatty acids, which makes them very sensitive to lipid peroxidation (Alahmar, 2019). Our previous studies have shown that lipid peroxidation plays a significant role in impairing sperm function and quality, particularly sperm count, motility, and morphology (Fafula & Vorobets, 2019;Vorobets et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Prooxidant-antioxidant balance in seminal and blood plasma of men with idiopathic infertility and infertile men in combination with rheumatoid arthritis

Фафула¹,

Melnyk²,

Gromnatska³

et al. 2023

Biol. Stud.

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Background. Male infertility is one of the most serious medical and social problems. Idiopathic infertility accounts for about 30 % of cases of infertile men. Rheumatoid arthritis is associated with a decreased fertility potential. The aim of the present work was to determine the lipid peroxidation level and the activity of antioxidant enzymes such as glutathione peroxidase and glutathione reductase in seminal plasma and blood plasma of infertile men with idiopathic infertility and concomitant autoimmune joint pathology (rheumatoid arthritis). Materials and Methods. 45 infertile men aged 22–48 were examined. They were divided into 2 groups: first group – 23 somatically healthy patients with idiopathic infertility; second group – 22 infertile men with rheumatoid arthritis. The control group consisted of 27 males with normal semen profile according to the WHO criteria and confirmed parenthood. The concentration of thiobarbituric acid reactive substance and activity of antioxidant enzymes were measured in the blood and seminal plasma. Results. When analyzing the seminal fluid, we found that the TBARS content was 4-fold greater in infertile men with autoimmune pathology compared to fertile men (p <0.001), whereas, in patients with idiopathic infertility its level was within the normal range. The activation of lipid peroxidation in infertile men with idiopathic infertility and in combination with rheumatoid arthritis was accompanied by a statistically significant decrease in the activity of enzymes of glutathione antioxidant system. It should be noted that more pronounced disorders of lipid peroxidation and antioxidant enzymes activity were found in seminal plasma compared to blood plasma. Conclusions. (1) An increased lipid peroxidation was observed in seminal and blood plasma of infertile men in combination with rheumatoid arthritis compared to normospermic men, whereas no differences were observed between men with idiopathic infertility and fertile men; (2) An impaired antioxidant status was observed in seminal and blood plasma of both men with idiopathic infertility and infertile men in combination with rheumatoid arthritis compared to normospermic men; (3) infertile men in combination with rheumatoid arthritis showed a significantly higher lipid peroxidation levels compared to men with idiopathic infertility, whereas no differences were observed in GPx and GR activity between groups.

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Section: Introductionmentioning

confidence: 99%

Prooxidant-antioxidant balance in seminal and blood plasma of men with idiopathic infertility and infertile men in combination with rheumatoid arthritis

Фафула¹,

Melnyk²,

Gromnatska³

et al. 2023

Biol. Stud.

Self Cite

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ATP-dependent ion-transport systems functioning in immunocompetent cells of men with erectile dysfunction due to combat trauma

Fafula,

Vorobets,

Vorobets

et al. 2024

Biol. Stud.

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Background. The study of the problem of physical and mental health of people who have survived combat trauma is a component of a wide field of research on the problem of stress, which manifests itself at all levels of the organization of the body. Combat injuries, in particular those of the areas of pelvis and genitourinary system, stressful events, lead to the development of erectile dysfunction (ED). In recent decades, a sufficient number of scientific facts have been accumulated, which confirm the significant influence of stress factors on the reduction of sexual desire and sexual activity. Therefore, determination of Ca2+,Mg2+- and Na+,K+-ATPase activity on a convenient model like peripheral blood lymphocytes add complexity to the understanding of the development of the pathophysiological and pathobiochemical mechanisms of the body, the result of which is the development of ED. Materials and methods. The research was conducted on peripheral blood lymphocytes of men injured as a result of combat operations (shrapnel and bullet wounds) in the Russian-Ukrainian war and treated at the Military Medical Clinical Center of the Western Region. The research group of men with combat injuries was divided into two subgroups: men aged 20–39 years (subgroup 1) and men aged 40–53 years (subgroup 2). The control group consisted of 48 practically healthy men without complaints of sexual dysfunction or cardiac, neurological or endocrinological pathology. Among the men of the control group were 30 men aged 20–39 years (subgroup 3) and 18 men aged 40–53 years (subgroup 4). Results. It has been shown that in the peripheral blood lymphocytes of men injured as a result of hostilities, there is a decrease in Na+,K+-ATPase activity and Ca2+,Mg2+-ATPase activity of the plasma membrane and endoplasmic reticulum, which leads to overloading of the cytosol with Na+ and Ca2+ ions, respectively, which is characteristic of pathological processes. Conclusion. Erectile dysfunction due to combat trauma is accompanied by a decrease in both Ca2+,Mg2+-ATPase activity of the plasma membrane and Ca2+,Mg2+-ATPase activity of endoplasmic reticulum of blood lymphocytes. As the age of patients with disorders of sexual function increases, the decrease in Ca2+,Mg2+-ATPase activities becomes more expressed. In men with erectile dysfunction due to combat trauma, the activity of Na+,K+-ATPase is also inhibited. According to the ROC curve, Ca2+,Mg2+-ATPase activity of the endoplasmic reticulum in blood lymphocytes is a potential biomarker of erectile dysfunction.

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The relationships between changes in main biochemical parameters in sperm cells of infertile men

Cited by 2 publications

References 18 publications

Prooxidant-antioxidant balance in seminal and blood plasma of men with idiopathic infertility and infertile men in combination with rheumatoid arthritis

Prooxidant-antioxidant balance in seminal and blood plasma of men with idiopathic infertility and infertile men in combination with rheumatoid arthritis

ATP-dependent ion-transport systems functioning in immunocompetent cells of men with erectile dysfunction due to combat trauma

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