The relative importance of the classical and alternative complement pathways in serum bactericidal activity against Escherichia coli

Roberts, Adrienne; Phillips, Ruth

doi:10.1099/00222615-16-1-69

Cited by 7 publications

(7 citation statements)

References 7 publications

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“…Possibly, differences in antibody titres may be responsible for this discrepancy. The CL of PMNL in the presence of MgEGTA confirmed observations of other authors that K -strains are more able to activate complement via the alternative pathway than K + strains (Roberts and Phillips, 1983). The differences between the CL induction by the K f and the K-strains in the presence of MgEGTA were in good agreement with the results of the complement consumption studies.…”

Section: Discussionsupporting

confidence: 82%

“…The kinetics of CL production strongly depended on the route of opsonisation, being delayed when the classical pathway of complement activation was blocked. The relatively slow opsonisation when serum is inactivated or when the alternative pathway alone is available for complement activation has been described by Tofte et al (1980) and by Roberts and Phillips (1983). Our results with zymosan suggested that a slow opsonisation resulted in a lower total CL response as a result of declining PMNL function by the time opsonisation was completed.…”

Section: Discussionsupporting

confidence: 57%

“…The slow rise in CL could be the result of ineffective opsonisation. It has already been shown that opsonisation via the alternative pathway of complement activation is a relatively slow process (Tofte et al, 1980;Roberts and Phillips, 1983). The low total activity of PMNL could be the result of this inefficient opsonisation, but may also be explained by an impairment of the PMNL by the time opsonisation is completed.…”

Section: Role Of Antibodies and Complement In Opsonisationmentioning

confidence: 99%

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The role of different K antigens of Escherichia coli in phagocytosis by polymorphonuclear leukocytes

Vught

Namavar

Peerbooms

et al. 1984

Journal of Medical Microbiology

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SUMMARY. The importance of K antigens from six strains of Escherichia coli for the interaction with polymorphonuclear leukocytes (PMNL) was studied. The major factor influencing this interaction was the ability of strains to activate complement by the classical route during opsonisation, this process being reduced for most K-positive strains. Interference of K antigens with the functioning of common pili as adhesins of eukaryotic cells was not observed nor a toxic effect of K antigens on PMNL.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 57%

Section: Role Of Antibodies and Complement In Opsonisationmentioning

confidence: 99%

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The role of different K antigens of Escherichia coli in phagocytosis by polymorphonuclear leukocytes

Vught

Namavar

Peerbooms

et al. 1984

Journal of Medical Microbiology

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“…This pooled serum (PNHS) was tested for the absence of anti-bacterial activity by agar diffusion with Staphylococcus aureus NCTC 657 1 and Escherichia coli NCTC 10418 control strains. Because the classical complement pathway has been shown to be important in the serum killing of Escherichia coli (Roberts and Phillips, 1983), the presence of somatic antibody to enterobacteria was tested in an indirect immunofluorescence test with E. coli and fluorescein labelled anti-human immunoglobulin (Borroughs Wellcome Ltd).…”

Section: Serummentioning

confidence: 99%

Bactericidal activity of human serum against strains of Klebsiella from different sources

Benge

1988

Journal of Medical Microbiology

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Summary.One hundred and eighty strains of Klebsiella were tested for sensitivity to pooled normal human serum, capsular type and faecal coliform reaction. Strains of K . pneumoniae isolated from clinical infections were more likely to be serum resistant than those from the gut flora or environmental sources. For K . oxytoca strains, there was no significant correlation between serum sensitivity and source of isolation. Of 60 strains of K . oxytoca tested, 19 (32%) were serum resistant, as were 27 (23%) of 120 strains of K . pneumoniae. Strains of K . pneumoniae from human sources gave positive reactions in the faecal coliform test more frequently than strains from environmental sources. There was no correlation between a positive faecal coliform reaction and resistance to the bactericidal effect of human serum. Strains of capsular type K21 were isolated more frequently from clinical infections and were more often serum resistant than other strains. Serum resistance appears to be a virulence factor in strains of K . pneumoniae but does not account for the difference in pathogenicity between K . pneumoniae and K . oxytoca.

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“…The extremely labile nature of nascent C3b (half‐life of approximately 60 μs 48 ) and low activation rates because of the bias toward inhibition of the pathway by FH and FI makes the “tickover” mechanism alone highly unlikely to effectively surveil pathogens. Therefore, it is no surprise that killing of most pathogens is either abrogated or considerably delayed when only the AP is active 49–58 …”

Section: Activation Of the Alternative Pathway On Microbesmentioning

confidence: 99%

Alternative pathway amplification and infections

Shaughnessy

Chabeda

Lewis

et al. 2022

Immunological Reviews

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The bactericidal activity of normal serum was described in the 1880s by von Fodor, 1 Nutall, 2 and Buchner. 3 Nutall showed that the bactericidal activity of sheep blood against Bacillus anthracis was lost when blood was heated to 55°C. Buchner coined the term alexin to describe the heat-labile factor responsible for bacterial killing. In 1894, Pfeiffer and Issaef 4 demonstrated that whole blood from guinea pigs that survived challenge with Vibrio cholerae could protect naive animals from developing disease. The following year, Jules Bordet showed that the bactericidal activity of heated immune serum could be restored by fresh serum that itself had no intrinsic bactericidal activity. 5 Thus, the bactericidal activity of immune serum was dependent on a heat-stable factor as well as a heat-labile factor (alexin). The combination of heat stable antibody (Ab) and alexin was shown to cause hemolysis. 6,7 Bordet and Gengou described complement fixation and showed the loss of complement activity from serum in the presence of antigen-Ab complexes. 8 The term "complement" was coined by Ehrlich. Bordet was awarded the Nobel Prize for Physiology or Medicine in 1919 for his "discoveries relating to immunity," including the discovery of complement activity and complement fixation tests.Work on the complement system till the 1950s focused largely on the classical pathway. Although not appreciated at the time, the existence of the alternative pathway (AP) was suggested by experiments where cobra venom factor and zymosan consumed complement activity without affecting the heat-labile complement components. 9,10 Pillemer and his coworkers first characterized the

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The relative importance of the classical and alternative complement pathways in serum bactericidal activity against Escherichia coli

Cited by 7 publications

References 7 publications

The role of different K antigens of Escherichia coli in phagocytosis by polymorphonuclear leukocytes

The role of different K antigens of Escherichia coli in phagocytosis by polymorphonuclear leukocytes

Bactericidal activity of human serum against strains of Klebsiella from different sources

Alternative pathway amplification and infections

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