“…The BM was diluted 1:8 in Dulbecco's phosphate-buffered saline (DPBS; Invitrogen Corporation, San Diego, CA) and the mononuclear cell (MNC) fraction was isolated by centrifugation over a 60% Percoll (Pharmacia, Uppsala, Sweden) density gradient at 500g for 30 minutes at room temperature (RT) [19]. The MNC fraction was collected and cultured in MSC growth media (MSCGM, Lonza, Inc., Walkersville, MD) at a density of 4,000 cells/cm 2 and allowed to proliferate.…”