The relaxant and spasmogenic effects of some xanthine derivatives acting on guinea‐pig isolated trachealis muscle

Abstract

Switzerland1 Caffeine (10mM)-induced relaxation of guinea-pig isolated trachealis was attenuated and converted to a small spasmogenic response on cooling to 220C. The relaxant response was restored on rewarming to 370C and was abolished by indomethacin (2.8 pM). Cooling to 220C in the presence of indomethacin revealed spasmogenic responses to caffeine which were abolished on rewarming to 370C.2 Trachealis treated with indomethacin (2.8upM) was repeatedly dosed with acetylcholine (ACh, 10pM). Caffeine (1 or 10mm), added as each ACh-induced spasm reached equilibrium, transiently augmented but then suppressed the spasm. On cooling from 370C to 12'C, the increment in spasm evoked by caffeine increased relative to the spasm evoked by ACh.3 Trachealis treated with indomethacin (2.8pM) was repeatedly dosed with caffeine (10mM). At 370C caffeine had little effect but it caused spasm when the tissue was cooled to 32°C. Spasm amplitude increased as cooling progressed to 12°C. Similar results were obtained with caffeine (1 mM).4 At 370C, caffeine, enprofylline, 1,3,7,9-tetramethylxanthinium (TMX), theobromine, theophylline, xanthine and forskolin each caused concentration-dependent suppression of tracheal tone. Among the xanthine derivatives the rank order of potency was enprofylline > theophylline > caffeine > theobromine > xanthine > TMX. 5 In trachealis treated with indomethacin (2.8pM) and maintained at 120C, the xanthines each caused concentration-dependent spasm. The rank order of potency was theobromine > theophylline > caffeine > enprofylline > xanthine > TMX. Forskolin was devoid of spasmogenic activity. 6 Trachealis treated with indomethacin (2.8 pM) and maintained at 120C was repeatedly dosed with either caffeine (10 mM) or potassium chloride (KCl, 40mM). Caffeine-induced spasm was attenuated in a Ca2"-free medium containing EGTA (2mM), modestly at first but subsequently more profoundly. KCl did not evoke spasm at 12°C but at 37°C the KCl-induced spasm was virtually abolished at its first trial in the Ca2 -free, EGTA-containing medium. 7 It is concluded that caffeine, other alkylated xanthines and xanthine itself share a spasmogenic action in guinea-pig isolated trachealis which is best observed when the tissue is treated with indomethacin (2.8 M) and maintained at 12°C. The spasmogenic action represents the release of Ca2+ from intracellular sites of sequestration and may not depend on the intracellular accumulation of cyclic AMP. The rank order of spasmogenic potency of the xanthine derivatives differs markedly from their rank order of potency in suppressing the spontaneous tone of the trachealis observed at 370C. Since, at 12°C, TMX is spasmogenic at concentrations identical to those causing relaxation at 37°C, it is likely that TMX penetrates the cell. The relaxant effects of TMX do not, therefore, indicate that methylxanthine-induced relaxation is mediated by a receptor located on the external surface of the cell.

Purinergic Signaling in the Airways

“…Adenosine relaxed guinea pig trachea via an A 2B receptor (Brown and Collis, 1982;Karlsson and Persson, 1984). Relaxation of the trachea is also produced by xanthines, but this is independent of adenosine antagonism (Darmani and Broadley, 1986;Small et al, 1988). Advenier et al (1988) suggested that epithelium had an influence on the mechanical response of guinea pig trachea to adenosine, but a later article (Thorne and Broadley, 2001) did not support this possibility.…”

Caffeine‐induced inhibition of calcium channel current in cultured smooth muscle cells from pregnant rat myometrium

“…In vascular smooth muscle, caffeine also releases cellular calcium to induce a transient contraction (Leijten & Van Breemen, 1984;Karaki et al, 1987). In addition to these contractile effects, caffeine has an inhibitory action in various smooth muscles, including myometrium (Ito & Kuriyama, 1971;Ashoori et al, 1985;Ahn et al, 1988;Small et al, 1988). Caffeine also inhibits cyclic adenosine 3',5'-monophosphate (cyclic AMP) phosphodiesterase leading to accumulation of intracellular cyclic AMP (Ahn et al, 1988;Bray et al, 1989) and decrease of cytoplasmic calcium concentration (Sato et al, 1988).…”

Inositol 1,4,5‐trisphosphate‐ and guanosine 5′‐O‐(3‐thio triphosphate)‐induced Ca2+ release in cultured airway smooth muscle

“…The methylxanthine, caffeine, is also well established as releasing intracellular Ca2 + from smooth muscle cells (Leijten & van Breemen, 1984;Benham & Bolton, 1986). In airway smooth muscle, caffeine can evoke contraction by liberation of intracellular Ca2+ (Ito & Itoh, 1984;Small et al, 1988).…”