The release of histamine by parasympathetic stimulation in guinea‐pig auricle and rat ileum.

Abstract

SUMMARY1. Two preparations, a segment of rat ileum and the vagally innerved guinea-pig auricles, have been used in an analysis of the responses to vagal or to electrical field stimulation.2. The responses to parasympathetic stimulation were depressed by atropine and by tetrodotoxin, and potentiated by eserine.3. Supramaximal stimulation (10-20 Hz) resulted in increased release of acetylcholine and histamine, both in rat ileum and guinea-pig auricles.4. The release of histamine after parasympathetic stimulation did not exhibit tachyphylaxis, and it was not reproduced by non-parasympathetic stimuli.5. In both preparations, atropine produced a significant, dose-related reduction of histamine measured in the bath fluid after stimulation, while eserine increased histamine output.6. A significant diminution of mast cell granules metachromasia was observed in guinea-pig auricles and in rat intestine after parasympathetic stimulation.7. The possibility is discussed that acetylcholine released by parasympathetic stimulation would in turn evoke the secretion of histamine from tissue mast cells.

Lack of evidence for histamine H 3 receptor function in rat ileum and human colon

“…It has been reported that histamine is released from mast cells in the intestinal wall of some species during electrical stimulation and hence, it may participate in the regulation of motor and secretory functions (Sacchi et al 1986). For example, the H 3 receptor antagonists thioperamide and impromidine have been reported to enhance electrically-evoked acetylcholine release in the guinea-pig ileum ).…”

Cardioprotective activity of endogenous and exogenous nitric oxide on ischaemia reperfusion injury in isolated guinea pig hearts

“…The different grey levels were obviously expressed in lower percentage values. The analysis of the image plan was carried out as previously described [33,34]. The program allowed for statistical and graphic elaboration of data.…”

Endogenous histamine excites neurones in the guinea‐pig superior cervical ganglion in vitro.

“…Although we activated mast cells with an immunological stimulus in this study, a variety of endogenous neurotransmitters and neuropeptides can release histamine from mast cells in other tissues (Foreman & Jordan, 1983;Bani-Sacchi, Barattini, Bianchi, Blandina, Brunelleschi, Fantozzi, Mannaioni & Masini, 1986;Leff, Stimler, Muhoz, Shioya, Tallet & Dame, 1986;Bienenstock, Tomioka, Matsuda, Stead, Quinonez, Simon, Coughlin & Denburg, 1987). The ability of these substances to act as mast cell secretagogues is dependent on the source of the mast cells, since considerable heterogeneity exists between mast cells from different tissues (Befus, Bienenstock & Denburg, 1986).…”