The Relevance of Genotyping and Subtyping of BVDV in Assessing the Epizootic Process

Bezborodova, N.A.; Vyalykh, I. V.; Tomskikh, O.G.

doi:10.17238/issn2072-6023.2018.4.69

Cited by 2 publications

(3 citation statements)

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“…The previously performed studies reveal that both non-cytopathogenic and cytopathogenic biotypes of BVDV circulated equally in the Sverdlovsk region (2016-2020) [1,5,8]. To determine the biotype of the pathogen circulating in cattle herds in 2022-2023, molecular genetic identification and differentiation of 11 isolates of BVDV was performed according to the method of Bezborodova N.A.…”

Section: Resultsmentioning

confidence: 99%

“…To determine the biotype of the pathogen circulating in cattle herds in 2022-2023, molecular genetic identification and differentiation of 11 isolates of BVDV was performed according to the method of Bezborodova N.A. et al [1]. The isolates were clinical samples of placenta (4 biomaterials), parenchymatous organ tissue homogenates of aborted foetuses (4 biomaterials), blood cell fraction (3 biomaterials) that contained BVDV genetic material.…”

Section: Resultsmentioning

confidence: 99%

“…Molecular genetic identification and differentiation of BVDV isolates were performed using reagent kits for DNA isolation "Diatom DNA Prep 200" (IsoGen LLC, Moscow), "Test-systems for detection of cattle VD by PCR" (LLC "Vetbiochem", Moscow), "Testsystems for detection of BVDV RNA by PCR with GFL-detection" ("AmpliSens", Moscow), specific oligonucleotide primers for three main regions of the BVDV genome: 5'-UTR, glycoprotein E2 gene, non-structural protein Npro gene; BLAST system and NCBI GenBank database [1].…”

Section: Methodsmentioning

confidence: 99%

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Problems of cattle health improvement and protection of population against viral diarrhoea under current conditions of animal husbandry

Klepova,

Poryvaeva,

Lysova

et al. 2024

BIO Web Conf.

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The article presents the dynamics of the prevalence of bovine viral diarrhoea virus (BVDV) in livestock enterprises of the Ural region in the period 2018-2023. Retrospective and operational analysis of monitoring data showed that the epizootic situation was consistently tense. The share of unfavourable livestock enterprises ranged from 18.2% to 33.3%. The cumulative infection rate in different age groups of cattle ranged from 2.3% to 23.3%. Clinical symptoms of acute course of BVDV-infection in examined cattle were registered in 10.9% of cases; persistent form of BVDV-infection - in 16.5% of cases; subclinical course of BVDV-infection and latent form - in 73.6% of cases. Phylogenetic analysis of BVDV isolates obtained from animal biosamples showed that 81.8% of isolates belong to the Cp-biotype of BVDV, 18.2% of isolates - to the Ncpbiotype of BVDV. It has been established that the intensity of pathogen eradication decreases in livestock enterprises when the regulations of specific prophylaxis are violated, as evidenced by the increase in the number of animals carrying BVDV by 4%; calves with diseases caused by BVDV infection - by 2.5%; cows and heifers with reproductive losses - by 5.7%.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Problems of cattle health improvement and protection of population against viral diarrhoea under current conditions of animal husbandry

Klepova,

Poryvaeva,

Lysova

et al. 2024

BIO Web Conf.

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Verification of a test system for detecting antibodies to bovine diarrhea virus using the enzyme immunoassay method “Cattle VD - SEROTEST plus”

Lobova,

Mikhailova,

Skvortsova

et al. 2024

Agrarnaâ nauka

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Relevance. Agricultural enterprises of the Russian Federation with intensive livestock farming are annually faced with the problem of a respiratory-intestinal nature, especially in young animals. This entails large losses. As a result of problems arising in animals, viral diarrhea of cattle occupies one of the leading places. The urgency of the problem lies in the large economic damage that occurs due to the birth of non-viable offspring, the death of newborn young animals, the development of various forms of pneumonia, decreased productivity, dysfunction of animal reproduction, as well as the costs of carrying out preventive, quarantine and liquidation measures. Reliable laboratory diagnostics allows you to correctly build a strategy to combat the disease. The article presents the results of verification of a test system for detecting antibodies to bovine diarrhea virus using the enzyme immunoassay method “Cattle VD — SEROTEST plus”.Methods. The diagnostic significance of the test system was assessed according to the indicators of sensitivity, specificity and precision under conditions of repeatability and reproducibility.Results. The coefficient of variation (CV) for the enzyme-linked immunosorbent assay ranged from 1.9 to 11.2%, indicating good consistency of results with intra-laboratory precision under conditions of repeatability and reproducibility.

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The Relevance of Genotyping and Subtyping of BVDV in Assessing the Epizootic Process

Cited by 2 publications

References 0 publications

Problems of cattle health improvement and protection of population against viral diarrhoea under current conditions of animal husbandry

Problems of cattle health improvement and protection of population against viral diarrhoea under current conditions of animal husbandry

Verification of a test system for detecting antibodies to bovine diarrhea virus using the enzyme immunoassay method “Cattle VD - SEROTEST plus”

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