The relevance of some laboratory tests in the diagnosis of cholestasis and the differentiation between intra- and extrahepatic obstruction

Kruiswijk, T.; Holtkamp, H.C.; Diaz, D.; Scidel, B.; Szakaly, M.

doi:10.1016/0009-8981(81)90451-4

Cited by 10 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 In different investigations, sensitivity and specificity ranged from 1.00 to 0.82 and from 0.99 to 0.74, respectively. 25,26,[29][30][31][32][33][34][35] All of these studies contain data from patients with various liver or biliary diseases and with intra-or extrahepatic cholestasis. Investigators reporting distinct Lp-X test results and histopathological evaluation of cholestasis in patients with cirrhosis found the following values for sensitivity and specificity: 0.85 and 0.96, 25 0.93 and 1, 26 or 1 and 0.64, 35 respectively.…”

Section: Discussionmentioning

confidence: 99%

Lipoprotein–X in Patients With Cirrhosis: Its Relationship to Cholestasis and Hypercholesterolemia

Sörös¹,

Böttcher²,

Maschek³

et al. 1998

Hepatology

View full text Add to dashboard Cite

Lipoprotein-X (Lp-X) is an abnormal low-density lipoprotein frequently found in liver disease. It is regarded as the most sensitive and specific biochemical parameter for the diagnosis of intra-and extrahepatic cholestasis. Moreover, Lp-X is supposed to contribute to the development of hypercholesterolemia in cholestatic liver disease, because it fails to inhibit de novo cholesterol synthesis. This investigation will focus on the relationship between the presence of Lp-X and serum lipid concentrations in cirrhosis. The significance of Lp-X in the diagnosis of cholestasis, compared with alkaline phosphatase (AP), ␥-glutamyl transferase (GGT), and bilirubin levels, will be assessed as well. The present cross-sectional study includes 212 patients with histopathologically proven cirrhosis. The detection of Lp-X and the quantification of ␣-, ␤-, and pre-␤-cholesterol was based on agar gel electrophoresis and polyanion precipitation. For the characterization of liver function, the concentrations of albumin and bilirubin, the activities of liver enzymes, and coagulation times were assessed. In a subgroup of 40 individuals, liver biopsies were re-evaluated to confirm or exclude intrahepatic cholestasis. As a result, there was no association between the appearance of Lp-X and total cholesterol concentrations. While all patients with Lp-X showed intrahepatic cholestasis (predictive value of the positive test Lipoprotein-X (Lp-X), an abnormal low-density lipoprotein, is present in patients with intra-or extrahepatic cholestasis, 1-3 in patients with familial lecithin:cholesterol acyltransferase (LCAT) deficiency, 4,5 and in newborn infants with immature liver function. 6 Lp-X is a lamellar particle of 30 to 70 nm in diameter as revealed by electron microscopy. It is characterized by its high content of phospholipids (66% by weight) and unesterified cholesterol (22%), and its low content of proteins (6%), cholesterol esters (3%), and triglycerides (3%). 7 The protein component is dominated by albumin, located in the core, and by apolipoprotein C, located on the surface of the particle. 8 Using zonal ultracentrifugation, Lp-X can be divided into three distinct populations: Lp-X 1 , Lp-X 2 , and Lp-X 3 , differing in density and apolipoprotein composition. 9 Unlike all other lipoproteins, Lp-X migrates to the cathode on agar gel electrophoresis. 7

show abstract

Section: Discussionmentioning

confidence: 99%

Lipoprotein–X in Patients With Cirrhosis: Its Relationship to Cholestasis and Hypercholesterolemia

Sörös¹,

Böttcher²,

Maschek³

et al. 1998

Hepatology

View full text Add to dashboard Cite

show abstract

“…Alcoholic and metastatic liver disease can also give ratios >10. Kruiswijk et al [133] examined GGT. its isoenzymes, and bile acids to differentiate extra-and intrahepatic obstruction.…”

Section: Cholestasismentioning

confidence: 99%

Serum Enzymes and Enzyme Profiles in the Diagnosis of Liver and Biliary Tract Disease

MacNamara¹,

Goldberg²

1985

Dig Dis

View full text Add to dashboard Cite

“…Εχει αναφερθεί ότι ο προσδιορισμός των χολικών οξέων στον ορό είναι η μόνη κλινική εξέταση από όλες τις άλλες, που επιτρέπει την στατιστική διαφοροποίηση των ασθενειών αυτών (61), ενώ προηγουμένως είχε αναφερθεί ότι οι ασθένειες αυτές διαφοροποιούνται μόνο με ηπατική βιοψία (62). Σε περιπτώσεις χολόστασης η διαγνωστική αξία του προσδιορισμού των χολικών οξέων είναι πολύ μεγάλη, δεν είναι δυνατή όμως η διαφοροποίηση ανάμεσα στην ηπατική και εξωηπατική χολόσταση (63). Σε περιπτώσεις αν ικτερικών ηπατικών ασθενειών (anicteric liver diseases), ενώ οι συγκεντρώσεις της χολερυθρίνης είναι τελείως φυσιολογικές, η διαγνωστική αξία του προσδ ιορισμού των χολικών οξέων είναι σημαντική (60,64), ειδικά όταν συνδυάζεται και με τον έλεγχο της μορφολογίας του ήπατος (65).…”

unclassified

Κινητικη Μελετη Και Αναλυτικες Εφαρμογες Ενζυμικων Συστηματων Για Τον Προσδιορισμο Χολικων Οξεων Σε Βιολογικα Υγρα

Λιανιδου¹

View full text Add to dashboard Cite

The relevance of some laboratory tests in the diagnosis of cholestasis and the differentiation between intra- and extrahepatic obstruction

Cited by 10 publications

References 11 publications

Lipoprotein–X in Patients With Cirrhosis: Its Relationship to Cholestasis and Hypercholesterolemia

Lipoprotein–X in Patients With Cirrhosis: Its Relationship to Cholestasis and Hypercholesterolemia

Serum Enzymes and Enzyme Profiles in the Diagnosis of Liver and Biliary Tract Disease

Κινητικη Μελετη Και Αναλυτικες Εφαρμογες Ενζυμικων Συστηματων Για Τον Προσδιορισμο Χολικων Οξεων Σε Βιολογικα Υγρα

Contact Info

Product

Resources

About