The Removal of β2-Microglobulin in Spent Dialysate Cannot Be Monitored by Spectrophotometric Analysis

Donadio, Carlo; Calia, D.; Ghimenti, Silvia; Onor, Massimo; Colombini, Elisa; Fuoco, Roger; Francesco, Fabio Di

doi:10.1159/000430906

Cited by 2 publications

(1 citation statement)

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“…Some weak emissions of non-modified β2M can be achieved with excitation at 220 nm, but such emissions of spent dialysate were found to be independent of the concentration of β2M in the dialysate leading to the conclusion that the β2M in spent dialysate cannot be directly monitored by spectrophotometric measurements [61]. Our spectra of the MM fraction of dialysate (Figure 7) confirm this statement regarding that neither the emission of glycated nor normal β 2 M are evidently dominant in the total fluorescence of spent dialysate at shorter excitation wavelengths when not corrected for the primary inner filter effect.…”

Section: Discussionmentioning

confidence: 99%

Optical Method and Biochemical Source for the Assessment of the Middle-Molecule Uremic Toxin β2-Microglobulin in Spent Dialysate

Paats

Adoberg

Arund

et al. 2021

Toxins

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Optical monitoring of spent dialysate has been used to estimate the removal of water-soluble low molecular weight as well as protein-bound uremic toxins from the blood of end stage kidney disease (ESKD) patients. The aim of this work was to develop an optical method to estimate the removal of β2-microglobulin (β2M), a marker of middle molecule (MM) uremic toxins, during hemodialysis (HD) treatment. Ultraviolet (UV) and fluorescence spectra of dialysate samples were recorded from 88 dialysis sessions of 22 ESKD patients, receiving four different settings of dialysis treatments. Stepwise regression was used to obtain the best model for the assessment of β2M concentration in the spent dialysate. The correlation coefficient 0.958 and an accuracy of 0.000 ± 0.304 mg/L was achieved between laboratory and optically estimated β2M concentrations in spent dialysate for the entire cohort. Optically and laboratory estimated reduction ratio (RR) and total removed solute (TRS) of β2M were not statistically different (p > 0.35). Dialytic elimination of MM uremic toxin β2M can be followed optically during dialysis treatment of ESKD patients. The main contributors to the optical signal of the MM fraction in the spent dialysate were provisionally identified as tryptophan (Trp) in small peptides and proteins, and advanced glycation end-products.

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Section: Discussionmentioning

confidence: 99%