The replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome

Kushwaha, Nirbhay Kumar; Mansi,; Chakraborty, Supriya

doi:10.1371/journal.ppat.1006587

Cited by 60 publications

(42 citation statements)

References 91 publications

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“…Viral proteins also routinely undergo PTMs to modulate infection. Recently, many geminiviral proteins (βC1, Rep, AC4, AV1) have been shown to undergo PTMs directly (Kushwaha et al, 2017;Shen et al, 2011;Li et al, 2018;Chowda-Reddy et al, 2008). We observed SyYVCV βC1 has multiple predicted SUMOylation consensus motifs as well as SIMs ( Figure 1A).…”

Section: Discussionmentioning

confidence: 52%

Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Nair

Chatterji

Vikram

et al. 2020

Preprint

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Statement: βC1 viral protein has evolved counter-defensive strategies to perturb host protein degradation pathways X. (2005). A Begomovirus DNA -Encoded Protein Binds DNA, Functions as a Suppressor of RNA Silencing, and Targets the Cell Nucleus. J. Virol. 79: 10764-10775. Dahlmann, B., Kuehn, L., Rutschmann, M., and Reinauer, H. (1985). Purification and characterization of a multicatalytic high-molecular-mass proteinase from rat skeletal muscle. Biochem.

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Section: Discussionmentioning

confidence: 52%

Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Nair

Chatterji

Vikram

et al. 2020

Preprint

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“…NbPI4KII was expressed as in‐frame fusion with the N‐terminal of the YFP protein using the pSPYNE vector. ChiLCV Rep protein was expressed with C‐terminal region of the YFP protein using pSPYCE vector (Kushwaha et al ., ). Bimolecular fluorescence complementation (BiFC) assays of interaction of (A) pSPYNE‐NbPI4KII and pSPYCE‐Rep, (B) pSPYNE‐ NbPI4KII and pSPYCE, (C) pSPYNE and pSPYCE‐Rep and (D) pSPYNE / pSPYCE.…”

Section: Resultsmentioning

confidence: 97%

“…To study the effect of ChiLCV Rep protein on the subcellular localization of NbPI4KII or vice versa, a co‐localization study was performed by co‐expressing NbPI4KII ‐mGFP and 35S: C1 ‐ DsRed . Nuclear localization of ChiLCV Rep‐DsRed has already been reported (Kushwaha et al ., ). The subcellular localization study revealed that Rep‐DsRed was confined to the nucleus in the presence of NbPI4KII‐mGFP.…”

Section: Resultsmentioning

confidence: 97%

“…There is 80% similarity between NbPI4KII and CaPI4KII, and the difference in their interacting abilities may be attributed to the difference in protein sequences. Additionally, as previously reported, different domains of ChiLCV‐encoded Rep protein are known to perform different functions (Orozco et al ., ) and these domains are differently localized in the host cell (Kushwaha et al ., ). The interaction between different Rep domains and NbPI4KII suggests an ability of this host protein to positively regulate Rep functions through interaction with all Rep domains and hence the infectivity of ChiLCV is enhanced.…”

Section: Discussionmentioning

confidence: 97%

“…The pSCPYCE-Rep, pSPYNE-NbPI4KII constructs were transformed into A. tumefaciens strain GV2260. BiFC assay was done by co-infiltration of pSPYCE-REP with pSPYNE-NbPI4KII into the lower epidermis of N. benthamiana following Kushwaha et al (2017). In vivo expression was observed under a confocal microscope (Model Eclipse TiE, Nikon, Tokyo, Japan).…”

Section: Bimolecular Fluorescence Complementation Assaysmentioning

confidence: 99%

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Nicotiana benthamiana phosphatidylinositol 4‐kinase type II regulates chilli leaf curl virus pathogenesis

Mansi

Kushwaha

Singh

et al. 2019

Molecular Plant Pathology

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Summary Geminiviruses are single‐stranded DNA viruses that can cause significant losses in economically important crops. In recent years, the role of different kinases in geminivirus pathogenesis has been emphasized. Although geminiviruses use several host kinases, the role of phosphatidylinositol 4‐kinase (PI4K) remains obscure. We isolated and characterized phosphatidylinositol 4‐kinase type II from Nicotiana benthamiana (NbPI4KII) which interacts with the replication initiator protein (Rep) of a geminivirus, chilli leaf curl virus (ChiLCV). NbPI4KII‐mGFP was localized into cytoplasm, nucleus or both. NbPI4KII‐mGFP was also found to be associated with the cytoplasmic endomembrane systems in the presence of ChiLCV. Furthermore, we demonstrated that Rep protein directly interacts with NbPI4KII protein and influenced nuclear occurrence of NbPI4KII. The results obtained in the present study revealed that NbPI4KII is a functional protein kinase lacking lipid kinase activity. Downregulation of NbPI4KII expression negatively affects ChiLCV pathogenesis in N. benthamiana. In summary, NbPI4KII is a susceptible factor, which is required by ChiLCV for pathogenesis.

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Functional role of geminivirus encoded proteins in the host: Past and present

Kamal,

Zafar,

Razzaq

et al. 2024

Biotechnology Journal

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During plant–pathogen interaction, plant exhibits a strong defense system utilizing diverse groups of proteins to suppress the infection and subsequent establishment of the pathogen. However, in response, pathogens trigger an anti‐silencing mechanism to overcome the host defense machinery. Among plant viruses, geminiviruses are the second largest virus family with a worldwide distribution and continue to be production constraints to food, feed, and fiber crops. These viruses are spread by a diverse group of insects, predominantly by whiteflies, and are characterized by a single‐stranded DNA (ssDNA) genome coding for four to eight proteins that facilitate viral infection. The most effective means to managing these viruses is through an integrated disease management strategy that includes virus‐resistant cultivars, vector management, and cultural practices. Dynamic changes in this virus family enable the species to manipulate their genome organization to respond to external changes in the environment. Therefore, the evolutionary nature of geminiviruses leads to new and novel approaches for developing virus‐resistant cultivars and it is essential to study molecular ecology and evolution of geminiviruses. This review summarizes the multifunctionality of each geminivirus‐encoded protein. These protein‐based interactions trigger the abrupt changes in the host methyl cycle and signaling pathways that turn over protein normal production and impair the plant antiviral defense system. Studying these geminivirus interactions localized at cytoplasm‐nucleus could reveal a more clear picture of host–pathogen relation. Data collected from this antagonistic relationship among geminivirus, vector, and its host, will provide extensive knowledge on their virulence mode and diversity with climate change.

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The replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome

Cited by 60 publications

References 91 publications

Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Nicotiana benthamiana phosphatidylinositol 4‐kinase type II regulates chilli leaf curl virus pathogenesis

Functional role of geminivirus encoded proteins in the host: Past and present

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