Understanding how humans perceive animals is important for biodiversity conservation, however, only a few studies about this issue have been carried out in South America. We selected two Brazilian cities to assess people’s perceptions of birds: Bauru (São Paulo, Brazil) and Belo Horizonte (Minas Gerais, Brazil). From the available bird data for each city, we developed a questionnaire and applied it between September 2020 and June 2021. The data obtained were analyzed by simple counts, a Likert scale, and percentages. Also, human feelings related to birds were placed on the Free Word Cloud Generator website. Our study confirmed that most respondents were aware of the importance of birds to ecological balance and that respondents had a generally positive attitude towards most of the bird species. However, they disliked exotic species such as the Domestic Dove and the House Sparrow, which are associated with disease, dirt, and disgust. Respondents also underestimated the number of birds that can live in urban areas and the song of birds is still a sense less experienced and perceived by people. Understanding these human–biodiversity relationships can help guide public policies and environmental education activities.