This study was carried out to investigate and verify the functionality of Artemisia argyi H. as cosmetic substances. Antimicrobial activity of water, 60% ethanol and 100% ethanol extracts showed inhibitory effects against Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, and Enterobacter aerogenes except Escherichia coli. As the results of MIC(Minimun inhibitory concentration) test, 60% ethanol extracts inhibited the growth of P. acnes at a concentration of 1.25 mg/mL. The mushroom tyrosinase inhibitory activity of water, 60% ethanol, and 100% ethanol extracts of A. argyi H. was increased in concentration-dependent manner. Melanin syntheses of B16/F10 mellanoma cells was also reduced by the extracts of A. argyi H. Especially, 60% ethanol extracts showed a excellent elastase inhibition of 51.9% at 125 ug/mL. In addition, the samples also showed a strong absorption in the range of UV-B(290-320nm). Overall, these results suggest that the extracts of A. argyi H. might be used for cosmetic industry such as anti-wrinkle or whitening substances.