The resistance mechanisms of b-lactam antimicrobials in clinical isolates of Acinetobacter baumannii

Kwon, Na Young; Kim, Jae Deok; Pai, Hyunjoo

doi:10.3904/kjim.2002.17.2.94

Cited by 14 publications

(11 citation statements)

References 18 publications

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“…The carbapenem-resistance genes (bla KPC , bla SME , bla IMI /bla NMC , bla GES , bla IMP , bla VIM , bla GIM , bla SIM-1 , bla SPM , bla NDM-1 , and bla OXA-48 ), common ESBL genes (bla CTX-M-1 , bla CTX-M-9 , bla TEM , and bla SHV ), and AmpC genes (bla MOX , bla FOX , bla DHA , bla CIT , and bla EBC ) were screened in all clinical strains using previously described primers (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). The positive PCR products were screened by electrophoresis on 1.0z agarose gel and were sequenced by Shanghai Majorbio Bio-Pharm Technology Co. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and Molecular Characteristics of Carbapenem-Non-Susceptible Enterobacteriaceae from a Teaching Hospital in Wenzhou, Southern China

et al. 2013

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SUMMARY: Carbapenem resistance in Enterobacteriaceae is increasing and has become a matter of great concern. The aim of this study was to characterize carbapenem-non-susceptible Enterobacteriaceae from a teaching hospital. A total of 49 carbapenem-non-susceptible Enterobacteriaceae clinical isolates recovered in 2007-2010 from the First Affiliated Hospital of Wenzhou Medical College were analyzed by antimicrobial susceptibility testing. The carbapenemase phenotype, outer membrane protein profiles, and clonal relatedness were investigated using the modified Hodge test, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and pulsed-field gel electrophoresis (PFGE). Multilocus sequence typing (MLST) of Klebsiella pneumoniae was also performed. b-Lactamase genes were examined by PCR and sequencing, and the transferability of carbapenemase genes was determined by a conjugation experiment. The rates of imipenem, meropenem, and ertapenem resistance were 59.2z, 40.8z, and 96.0z, respectively. Thirty isolates exhibited carbapenemase activity, and 32 isolates carried carbapenemase genes. Furthermore, 10 and 9 clinical isolates posessed AmpC b-lactamase and extended-spectrum b-lactamase (ESBL) genes, respectively. Eight of 32 carbapenemase-producing isolates were proved to be carried by conjugative plasmids, and there was porin loss in 34.7z (17/49) of the isolates. PFGE analysis demonstrated that 9 KPC-2-producing Serratia marcescens belonged to a clonal strain, suggesting the clonal dissemination of these KPC-2-bearing isolates among different wards. The MLST of K. pneumoniae revealed that two KPC-2 producers were ST11. This study suggests that KPC-2-type carbapenemase is the main contributor to carbapenems resistance in carbapenemase-producing Enterobacteriaceae, and that ESBL, AmpC b-lactamase overproduction, and porin loss contribute to the resistance level among these isolates; in carbapenemase-non-producing Enterobacteriaceae, ESBL, AmpC enzyme, and porin loss contribute to the carbapenems resistance of Enterobacteriaceae, especially the ertapenem resistance of Enterobacter cloacae.

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Section: Methodsmentioning

confidence: 99%

Phenotypic and Molecular Characteristics of Carbapenem-Non-Susceptible Enterobacteriaceae from a Teaching Hospital in Wenzhou, Southern China

et al. 2013

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“…The class A ␤-lactamases PER-1 and VEB-1, which are genetically distant from the predominant TEM-and SHV-derived ESBLs and CTX-M-type enzymes, were first reported among nosocomial A. baumannii isolates in Turkish and French hospitals (41,52). Similar strains have subsequently been isolated in France (40) and Korea (26). Among the CTX-M-type ␤-lactamases, the CTX-M-5 gene has been found in A. baumannii (GenBank accession number AF462635); however, no details have been published to date.…”

Section: Vol 42 2004mentioning

confidence: 97%

“…Various ␤-lactamases demonstrating broad-spectrum substrate specificities that allow A. baumannii to cope with broad-spectrum ␤-lactams, such as OXA-type class D ␤-lactamases (19,39,53), metallo-␤-lactamases belonging to class B ␤-lactamases (45,51,57), and AmpC-type class C ␤-lactamases (7), have been found in A. baumannii so far. Moreover, PER-1 and VIB-1 class A ␤-lactamases demonstrating broad-spectrum substrate specificities have also recently been detected among nosocomially isolated A. baumannii strains in Turkey (52) and were then detected in France (40,41) and Korea (26).…”

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confidence: 99%

Nosocomial Transmission of CTX-M-2 β-Lactamase-Producing Acinetobacter baumannii in a Neurosurgery Ward

Nagano

Cordevant

et al. 2004

J Clin Microbiol

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Three strains of cefotaxime (CTX)-resistant Acinetobacter baumannii, FM0209680, FM0300106, and FM0301433, were isolated from transtracheal aspirate cultures of three patients with probable nosocomial infections in a neurosurgery ward in Japan. The CTX MICs for these isolates were greater than 128 g/ml but were drastically reduced in the presence of 4 g of clavulanic acid per ml. These strains were also resistant to ceftriaxone, cefpodoxime, and aztreonam but were susceptible to ceftazidime and imipenem. The profile of resistance to various broad-spectrum ␤-lactams was transferred by conjugation. Strain FM0209680 was not eradicated from case patient 1 by administration of imipenem, ceftazidime, and levofloxacin, even after a 6-month hospitalization period. Strains FM0300106 and FM0301433 were isolated from case patients 2 and 3 during the sixth week following admission, respectively, and then each patient was colonized for 3 weeks. Eradication of FM0300106 was successfully obtained from case patient 2 by imipenem treatment, while administration of imipenem was continued to prevent pneumonia. Prophylactic antimicrobial therapy was discontinued in case patient 3 because of the lack of pneumonic symptoms, and FM0301433 disappeared after the discontinuation of antimicrobial chemotherapy. All three strains carried the bla CTX-M-2 gene, and the appearance of colonies in the growth-inhibitory zones around disks of CTX and aztreonam in double-disk synergy tests suggested inducible ␤-lactamase production in these A. baumannii strains. The ribotyping investigation suggested that all these strains belong to the same clonal lineage. The plasmids harbored by A. baumannii had the same restriction profile as those harbored by Proteus mirabilis strains previously isolated in a urology ward of the Funabashi Medical Center.

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“…12 Up-regulation of congenital resistance mechanisms and exogenous resistance are the main causes of multi-drug resistant (MDR) A. baumannii. [13][14][15][16] Various drug-resistance mechanisms can be combined with each other to lead to the development of highly resistant and widely resistant strains. 15 The diversity and complexity of antibiotic-resistant mechanisms and the undetermined mechanisms of drug resistance pose a great challenge to solve the problem of antibioticresistance of A. baumannii.…”

Section: Introductionmentioning

confidence: 99%

<p>Trends and Development in the Antibiotic-Resistance of <em>Acinetobacter baumannii</em>: A Scientometric Research Study (1991–2019)</p>

Liu¹,

Wu²,

Tang³

et al. 2020

IDR

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Objective: Data visualization software were used to display and analyze the research status, hotspot and development trend of the antibiotic-resistance of Acinetobacter baumannii objectively and comprehensively, so as to provide guidance and reference for the research of the antibiotic-resistant Acinetobacter baumannii. Materials and Methods: The data of relevant publications on antibiotic-resistant Acinetobacter baumanii from 1991 to 2019 were retrieved from Web of Science (WOS) Core database. VOSviewer and CiteSpace software were used to conduct co-citation visualization network rendering and cluster analysis on the publications' years, authors, countries, institutions, keywords and citations. Results: A total of 3915 valid records on the study of antibiotic-resistant Acinetobacter baumanii were retrieved. The number of relevant publications was increasing year after year. The United States is the most influential country in the field, which works closely with other countries and publishes most of the papers. University of Sydney is the leading institution in this area. Bonomo Robert A publishes most of the papers. There are the highest number of publications in the research areas of antimicrobial agents and chemotherapy. "Nucleotide sequence" and "outbreak" were once the hotspots in this field, but recently "bacteriophage", "biofilm" and "colistin resistance" have become the research hotspots. Conclusion: Since 1991, the number of publications on antibiotic-resistant Acinetobacter baumannii has grown rapidly, and various countries and institutions have paid close attention to the problem of antibiotic resistance. Countries, institutions and researchers, which have strong influential power, collaborate with each other closely. The future research direction of antibiotic-resistant Acinetobacter baumannii should lie in the further breakthrough of antibacterial peptides, bacteriophage therapy, CRISPR system and various combined therapies.

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The resistance mechanisms of b-lactam antimicrobials in clinical isolates of Acinetobacter baumannii

Cited by 14 publications

References 18 publications

Phenotypic and Molecular Characteristics of Carbapenem-Non-Susceptible Enterobacteriaceae from a Teaching Hospital in Wenzhou, Southern China

Phenotypic and Molecular Characteristics of Carbapenem-Non-Susceptible Enterobacteriaceae from a Teaching Hospital in Wenzhou, Southern China

Nosocomial Transmission of CTX-M-2 β-Lactamase-Producing Acinetobacter baumannii in a Neurosurgery Ward

<p>Trends and Development in the Antibiotic-Resistance of <em>Acinetobacter baumannii</em>: A Scientometric Research Study (1991–2019)</p>

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