The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity

Coulibaly, Ibourahema; Dubois-Dauphin, Robin; Destain, Jacqueline; Fauconnier, Marie‐Laure; Lognay, Georges; Thonart, Philippe

doi:10.1155/2010/625239

Cited by 25 publications

(15 citation statements)

References 43 publications

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“…However, during this low-temperature desiccation process, bacteria are exposed to several environmental stresses (thermal, osmotic, water removal, and oxidative stresses), which could induce major cellular damages, leading to loss of viability and functional activities (acidification, production of aroma compounds and texturizing agents, etc.) (Coulibaly et al 2010;Velly et al 2014). Damages to cellular systems resulting from freezedrying can be attributed to two major causes: changes in the physical state of cytoplasmic membrane lipids, resulting in a loss of bacterial membrane integrity (Crowe et al 1989a;Linders et al 1997;Schwab et al 2007), and changes in the structure of sensitive proteins (Bischof et al 2002;Oldenhof et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Cyclopropanation of unsaturated fatty acids and membrane rigidification improve the freeze-drying resistance of Lactococcus lactis subsp. lactis TOMSC161

Velly¹,

Bouix²,

Passot³

et al. 2014

Appl Microbiol Biotechnol

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This work aimed at characterizing the biochemical and biophysical properties of the membrane of Lactococcus lactis TOMSC161 cells during fermentation at different temperatures, in relation to their freeze-drying and storage resistance. Cells were cultivated at two different temperatures (22 and 30°C) and were harvested at different growth phases (from the middle exponential phase to the late stationary phase). Bacterial membranes were characterized by determining the fatty acid composition, the lipid phase transition, and the membrane fluidity. Cultivability and acidification activity losses of L. lactis were quantified after freezing, drying, and 3 months of storage. The direct measurement of membrane fluidity by fluorescence anisotropy was linked to lipid composition, and it was established that the cyclopropanation of unsaturated fatty acids with concomitant membrane rigidification during growth led to an increase in the freeze-drying and storage resistance of L. lactis. As expected, cultivating cells at a lower fermentation temperature than the optimum growth temperature induced a homeoviscous adaptation that was demonstrated by a lowered lipid phase transition temperature but that was not related to any improvement in freezedrying resistance. L. lactis TOMSC161 was therefore able to develop a combined biochemical and biophysical response at the membrane level during fermentation. The ratio of cyclic fatty acids to unsaturated fatty acids (CFA/UFA) appeared to be the most relevant parameter associated with membrane rigidification and cell resistance to freeze-drying and storage. This study increased our knowledge about the physiological mechanisms that explain the resistance of lactic acid bacteria (LAB) to freeze-drying and storage stresses and demonstrated the relevance of complementary methods of membrane characterization.

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Section: Introductionmentioning

confidence: 99%

Cyclopropanation of unsaturated fatty acids and membrane rigidification improve the freeze-drying resistance of Lactococcus lactis subsp. lactis TOMSC161

Velly¹,

Bouix²,

Passot³

et al. 2014

Appl Microbiol Biotechnol

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“…Microencapsulation has been proven as an effective method for protecting probiotic bacteria during freeze drying (Lee and others 2004; Higl and others 2007; Tsen and others 2007; Kurtmann and others 2009; Coulibaly and others 2010; Heidebach and others 2010). Among hydrocolloids as coating materials, calcium alginate in the form of gel beads has been widely used for the immobilization of probiotic bacteria (Louis and others 1990; Lee and Heo 2000) due to its easy handling, nontoxic nature, low cost, gentle process condition, and easy to dissolve in intestine thus releasing entrapped cells (Sultana and others 2000; Reid and others 2005; Anal and Singh 2007; Mortazavian and others 2007).…”

Section: Introductionmentioning

confidence: 99%

Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Dianawati

Shah

2011

Journal of Food Science

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Survival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 °C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl(2) solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a(w) improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a(w) of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a(w) adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%).

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“…Then, the major obstacle in the commercialization of biocontrol products is the development of a shelf‐stable formulated product with biocontrol activity similar to the fresh cells of the agent (Droby et al. 2009; Coulibaly et al. 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Then, the major obstacle in the commercialization of biocontrol products is the development of a shelf-stable formulated product with biocontrol activity similar to the fresh cells of the agent (Droby et al 2009;Coulibaly et al 2010). Freeze-drying is the most convenient and successful method of preserving bacteria, yeast and fungi (Morgan et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Freezing and freeze-drying of the bacterium Rahnella aquatilis BNM 0523: study of protecting agents, rehydration media and freezing temperatures

Navarta

Calvo

Calvente

et al. 2011

Letters in Applied Microbiology

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Aims: The aim of the present study was to evaluate and compare freezing and freeze‐drying treatments for conserving Rahnella aquatilis (BNM 0523) with the goal to achieve an adequate commercial formulation of this biocontrol agent. Methods and Results: The effect of several protective agents, rehydration media and freezing temperatures on the viability and functional activity of the R. aquatilis was investigated. The storage stability at 3 months and 4 years was determined by checking the viability of the cells and their biocontrol capability against Botrytis cinerea by measuring the percentage of reduction of disease severity on apple. The best results were obtained by the freeze‐drying of the cells using a mixture of skimmed nonfat milk 10%, yeast extract 0·5% and glucose 1% as the protecting and rehydrating medium, and a quickly freezing (−70°C) before the freeze‐drying. In this case, the viability of the cells after 4 years was 98%, and their antagonistic ability showed a little decrease with respect fresh cells. Conclusions: The studies showed that R. aquatilis was resistant to freezing and freeze‐drying when it was used a mixture of cryoprotectants and that it was possible to obtain inoculums with high viability and good effectiveness for reduction of decay caused by B. cinerea. Significance and Impact of the study: This study is probably the first report about the resistance of R. aquatilis to freezing and freeze‐drying treatments and shows that these operations could be useful for obtaining a commercial formulation of this biocontrol agent.

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The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity

Cited by 25 publications

References 43 publications

Cyclopropanation of unsaturated fatty acids and membrane rigidification improve the freeze-drying resistance of Lactococcus lactis subsp. lactis TOMSC161

Cyclopropanation of unsaturated fatty acids and membrane rigidification improve the freeze-drying resistance of Lactococcus lactis subsp. lactis TOMSC161

Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

Freezing and freeze-drying of the bacterium Rahnella aquatilis BNM 0523: study of protecting agents, rehydration media and freezing temperatures

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