The Resolution of Dye Binary Mixtures by Bivariate Calibration Using Spectrophotometric Data

Abstract

A new, simple spectrophotometric method for resolution of dye binary mixtures is proposed in this work. A simple mathematical algorithm was designed, in which the data are used from four linear regression calibration equations: two calibrations for each component at two selected wavelengths. The method of Kaiser was applied for the selection of the optimum two-wavelength sets for all mixtures under study. The recovery experiments were carried out in ten mixtures of the following dyes: Tartrazine, Amaranth, Erythrosin B, Sunset Yellow and Allura Red. The obtained results were compared with the results of a commonly used derivative spectrophotometric procedure (zero-crossing technique). The statistical evaluation of the method bias was performed and it was concluded that the proposed methodoloa may be competitive with the derivative procedure for the resolution of such dye binary mixtures.

Simultaneous UV Spectrophotometric Determination of Pravastatin Sodium and Pioglitazone Hydrochloride in Pharmaceutical Preparations

“…Several manipulations were performed to enable mixture resolution for example, using different order derivatives 8,9 , derivatives of the ratio spectra 10,11 , ratio subtraction technique 13 , and isoabsorptive method 14 . Many other methods which depend on measuring the absorbance at two wavelengths, include, dual wavelength method 15 , ratio difference method 16 , H-point standard addition method 17 , bivariate method 18 , and absorbance ratio method 19 .…”

A comparative study between three stability indicating spectrophotometric methods for the determination of diatrizoate sodium in presence of its cytotoxic degradation product based on two-wavelength selection

“…Unlike the other two methods, the ratio difference method allows the selection of any two wavelengths for determination of the drug, such as ϭϯ wavelengths of maximum absorbance that can increase the sensitivity of the method. In bivariate method, upon applying Kaiser method [21] for selecting the wavelengths for bivariate method, wavelengths that has low sensitivity may be introduced as shown in the regression equation for DTA at 264 nm. The dual wavelength method has a main disadvantage that the selected wavelengths often meet at peak shoulders, where ∆A/∆λ is said to be maximum; therefore the selectivity towards the measured component is extremely affected.…”

“…This method is based on the simple mathematic algorithm [21], in which data are used from four linear regression equations, two calibrations for each component at two selected wavelengths using the method of Kaiser [22]. The Six wavelengths were taken and the slope values of the linear regression equations were estimated for DTA and the degradate at the selected wavelengths.…”

Determination of Fenofibrate and the Degradation Product Using Simultaneous UV-Derivative Spectrometric Method and HPLC

“…1 H NMR spectrum of fenofibrate in (Figure 2 For the selection of the two most appropriate wavelengths with respect to their sensitivity for the simultaneous determination of the substances, we applied Kaiser's [28][29][30] method, which consists of resolving the determinant from the so called selectivity matrix, K.  , correspond to the sensitivity parameters of component B, in this case considered as calibration curve slopes for each component at two given wavelengths. 13 C NMR spectra of fenofibrate and its degraded products in (Figures 3(a) & 3(b)) show identical carbon peaks except in the degraded product there is a complete disappearance of carbon peaks at δ 21.52 and at 69.34 corresponding to the aliphatic carbon of the two methyl groups of [-O-CH-(CH 3 ) 2 ] and the methine carbon of [-O-CH-(CH 3 ) 2 ], respectively.…”