The Respiration of Luminous Bacteria and the Effect of Oxygen Tension Upon Oxygen Consumption

Abstract

1. The respiration of luminous bacteria has been studied by colorimetric and manometric methods. 2. Limulus oxyhaemocyanin has been used as a colorimetric indicator of oxygen consumption and indicator dyes were used for colorimetric determination of carbon dioxide production. 3. The Thunberg-Winterstein microrespirometer has been used for the measurement of the rate of oxygen consumption by luminous bacteria at different partial pressures of oxygen. 4. The effect of oxygen concentration upon oxygen consumption has been followed from equilibrium with air to low pressures of oxygen. 5. Luminous bacteria consume oxygen and produce carbon dioxide independent of oxygen pressures from equilibrium with air (152 mm.) to approximately 22.80 mm. oxygen or 0.03 atmosphere. 6. Dimming of a suspension of luminous bacteria occurs when oxygen tension is lowered to approximately 2 mm. Hg (0.0026 atmosphere) and when the rate of respiration becomes diminished one-half. 7. Pure nitrogen stops respiratory activity and pure oxygen irreversibly inhibits oxygen consumption. 8. The curve for rate of oxygen consumption with oxygen concentration is similar to curves for adsorption of gasses at catalytic surfaces, and agrees with the Langmuir equation for the expression of the amount of gas adsorbed in unimolecular layer at catalytic surfaces with gas pressure. 9. A constant and maximum rate of oxygen consumption occurs in small cells when oxygen concentration becomes sufficient to entirely saturate the surface of the oxidative catalyst of the cell.

Linkage Between Output of Electric Energy by Polar Tissues and Cell Oxidation

“…of Douglas fir when the temperature was restored to its original value after a period of exposure to low temperature (18,19). Furthermore, the rebound effect is also similar to the increase in velocity of oxidation, observed by a number of investigators, when certain tissues and organisms were exposed to oxygen after a period in its absence; nerve (6,5), muscle (5), tomato fruits (7), Planaria (11), and luminous bacteria (25,8). GuSTAFSON (7) also found that, in a given tomato fruit, the magnitude and continuance of increased velocity of oxidation when air was reintroduced into the chamber were dependent upon the length of the period of exposure to pure hydrogen or pure nitrogen.…”

Diffusion and Monod kinetics model to determine in vivo human corneal oxygen-consumption rate during soft contact lens wear

“…If we take the extreme values of 12 or 25 mmHg for the oxygen partial pressure (Shoup, 9 Amberson, 10 Fatt, 11 Tang, 12 Takahashi et al 13 ), other values for K m could have been possible. Nevertheless, the Chhabra's value for K m tends to be an estimated average value, and in this way it may be perfectly acceptable.…”

Diffusion and Monod kinetics to determine in vivo human corneal oxygen‐consumption rate during soft contact‐lens wear

“…This technique provides a simple, fast, reliable, and noninvasive way to obtain Q c by wearing a soft contact lens for a few minutes 8. Unfortunately, the current mathematical framework used to obtain Q c from the measured tear‐film oxygen tension is not satisfactory because the oxygen‐consumption rate is assumed constant throughout the cornea; the oxygen‐consumption rate, however, is known to be a nonlinear function of local oxygen tension in biological tissues and organisms 13–21. Further, the corneal oxygen permeability ( D c k c ) needed for obtaining human Q c was measured on rabbits for two corneal layers: endothelium and epithelium 8, 22, 23.…”